The Effectiveness of Integrated Management Packages in Controlling Aphid-Borne Virus Infections on Groundnut in South Sulawesi, Indonesia

Niken Nur Kasim, Tri Asmira Damayanti, Sri Hendrastuti Hidayat, Sugeng Santoso


Aphid-borne viral infection is a major limiting factor for groundnut production in South Sulawesi, Indonesia. The study aimed to elucidate the effectiveness of integrated management packages (IMP) in controlling aphid-borne viruses infecting groundnut in the field. Field trials were conducted in a plot without (M0) and with plastic mulch and maize as a plant barrier (M1), while two different groundnut varieties, Bougainvillea leaf extract, and insecticides were incorporated as the subplot treatments. The treatments in M1 plot significantly reduced the aphid population (17.4–79.9%) and AUDPC incidence (51.7–87.3%), and severity (61.0–92.9%), respectively. The treatments increased dry pod production by 11.9–63.3% depending on subplot treatment compared to treatments in the M0 plot (p>0.05). Bean common mosaic virus strain PSt and Cowpea Polerovirus 2 were positively detected in groundnuts as well as in aphids using RT-PCR. Further, nine weed species in the vicinity of the field trial were found to be positively infected with BCMV-PSt, indicating their crucial role as reservoir hosts. Interestingly, the effectiveness of Bougainvillea leaf extract treatment in the M1 plot was comparable to that of the insecticide treatment, indicating that this IMP is a more cost-effective, eco-friendly groundnut virus control than the currently used method.


Barrier crops; Bougainvillea; Integrated pest management (IPM); Plastic mulch; Weeds

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Abraham, A. D., Menzel, W., Lesemann, D.-E., Varrelmann, M., & Vetten, H. J. (2006). Chickpea chlorotic stunt virus: A New Polerovirus Infecting Cool-Season Food Legumes in Ethiopia. Phytopathology, 96(5), 437–446.

Andayanie, W. R. (2012). Diagnosis penyakit mosaik (soybean mosaic virus) terbawa benih kedelai. Journal of Tropical Plant Pests and Diseases, 12(2), 185–191.

Barzman, M., Bàrberi, P., Birch, A. N. E., Boonekamp, P.M., Dachbrodt-Saaydeh, S., Graf, B., Hommel, B., Jensen, J. E., Kiss, J., Kudsk, P., Lamichhane, J. R., Messéan, A., Moonen, A. C., Ratnadass, A., Ricci, P., Sarah, J. L., & Sattin, M. (2015). Eight principles of integrated pest management. Agronomy for Sustainable Development, 35(4), 1199-1215.

Batzer, J. C., Singh, A., Rairdin, A., Chiteri, K., & Mueller, D. S. (2022). Mungbean: A preview of disease management challenges for an alternative U.S. cash crop. Journal of Integrated Pest Management, 13(1), 1-21.

Bhat, A. I., Jain, R. K., Varma, A., & Lal, S. K. (2002). Nucleocapsid protein gene sequence studies suggest that soybean bud blight is caused by a strain of groundnut bud necrosis virus. Current Science, 82(11), 1389–1392.

Biondi, A., Guedes, R. N. C., Wan, F. H., & Desneux, N. (2018). Ecology, worldwide spread, and management of the invasive South American tomato pinworm, Tuta absoluta: Past, present, and future. Annual Review of Entomology, 63, 239–258.

Budiono. (2001). Uji kisaran inang Peanut Stripe Virus (PStV) pada gulma famili Leguminosae dan potensinya sebagai sumber inokulum dengan vektor Aphis craccivora Koch. pada tanaman kacang tanah [Undergraduate Thesis, Universitas Jember].

Cahyati, I., Damayanti, T. A., & Santoso, S. (2023). Combination of barrier crop, chitosan, Bougainvillea leaf extract to manage virus on Soybean. Jurnal Fitopatologi Indonesia, 19(4), 166–175.

Choi, S. K., Choi, J. K., Park, W. M., & Ryu, K. H. (1999). RT-PCR detection and identification of three species of Cucumoviruses with a genus-specific single pair of primers. Journal of Virological Methods, 83(1-2), 67-73.

Choudhary, N., Kapoor, H. C., & Lodha, M. L. (2008). Cloning and expression of antiviral/ribosome-inactivating protein from Bougainvillea xbuttiana. Journal of Biosciences, 33(1), 91–101.

Damayanti, T. A., & Naidu, R. A. (2009). Identification of Peanut bud necrosis virus and Tomato spotted wilt virus in Indonesia for the first time. Plant Pathology, 58(4), 782-782.

Damayanti, T.A., Alabi, O.J., Naidu, R.A., & Rauf, A. (2009). Severe outbreak of a yellow mosaic disease on the yard long bean in Bogor, West Java. Hayati Journal of Biosciences, 16 (2), 78–82.

Damayanti, T. A., & Panjaitan, M. T. (2014). Aktivitas antivirus beberapa ekstrak tanaman terhadap Bean common mosaic virus strain Blackeye Cowpea (BCMV-BIC) pada kacang panjang. Journal of Tropical Plant Pests and Diseases, 14(1), 32–40.

Damayanti, T. A., & Pebriyeni, L. (2015). Tanaman penghalang dan ekstrak daun pagoda untuk mengendalikan Bean common mosaic Vvrus pada kacang panjang di lapangan. Jurnal Hortikultura, 25(3), 238-245.

Damayanti, T. A., & Wiyono, S. (2015). Genetic diversity of Cucumber mosaic virus strain soybean from several areas. Microbiology Indonesia, 9(1), 44–49.

Damicone, J. P., Edelson, J. V., Sherwood, J. L., Myers, L. D., & Motes, J. E. (2007). Effects of border crops and intercrops on control of Cucurbit virus diseases. Plant Disease, 91(5), 509–516.

de Breuil, S., Giolitti, F.J., & Lenardon, S.L. (2005). Detection of Cucumber mosaic virus on peanut (Arachis hypogaea L.) in Argentina. Journal of Phytopathology, 153(11–12), 722-725. http://doi:10.1111/j.1439-0434.2005.01028.x

de Breuil, S., Giolitti, F. J., Bejerman, N., & Lenardon, S. L. (2012). Effects of Cucumber mosaic virus on the yield and yield components of peanut. Journal of Plant Pathology, 94(3), 669–673.

de Breuil, S., Nome, C. F., Flores, C., Bejerman, N., Giolitti, F., Trucco, V., & Lenardon, S. (2015). First Report of Bean common mosaic virus, Peanut Strain, Infecting Peanut in Argentina. Plant Disease, 99(5), 735–735.

Dolja, V.V., Krupovic, M., & Koonin, E.V. (2020). Deep roots and splendid boughs of the global plant virome. Annual Review of Phytopathology, 58, 23–53.

Doyle, J.J. & Doyle, J.L. (1987) A Rapid DNA Isolation Procedure for Small Quantities of Fresh Leaf Tissue. Phytochemical Bulletin, 19, 11-15.

Drijfhout,E., & Morales, F. (2005). Bean Common Mosaic. in: Schwartz HF, Steadman JR, Hall R, Forster RL, editor. Compendium of Bean Diseases, 2nd ed. Saint Paul (MN): American Phytopathological Society.

Elwakil, M.A., & El-Metwally, M.A. (2001). Seed-borne fungi of peanut in Egypt: pathogenicity and transmission. Pakistan Journal of Biological Sciences, 4(1), 63-68.

Fereres, A. (2000). Barrier crops as a cultural control measure of non-persistently transmitted aphid-borne viruses. Virus Research, 71(1-2), 221-231.

Gatan, M.T.. Gatan, M.G.B. (2020). Effect of Planting Date on Disease Occurrence and Yield of Three Peanut Varieties. SSRN Electronic Journal, 1-9.

Ghewande, M.P., & Nandagopal, V. (1997). Integrated pest management in groundnut (Arachis hypogaea L.) in India. Integrated Pest Management Reviews, 2, 1–15.

Ghewande, M.P., Desai, S., Narayan, P., & Ingle, A. P. (1993). Integrated management of foliar diseases of groundnut (Arachis hypogaea L.) in India. International Journal of Pest Management, 39(4), 375–378.

Güller, A., Sipahioğlu, H. M., Usta, M., & Durak, E. D. (2018). Antiviral and antifungal activity of biologically active recombinant Bouganin Protein from Bougainvillea spectabilis Willd. Journal of Agricultural Sciences, 24, 227-237.

Higgins, C. M., Cook, G., Pietersen, G., & Dietzgen, R. G. (1999). Molecular characterisation of a strain of peanut stripe potyvirus from groundnut germplasm imported into South Africa. African Plant Protection, 5(1), 5-12.

Hooks, C. R. R., & Fereres, A. (2006). Protecting crops from non-persistently aphid-transmitted viruses: A review on the use of barrier plants as a management tool. Virus Research, 120(1–2), 1–16.

Indonesian Ministry of Agriculture. (2021). Laporan Tahunan Direktorat Jendral Tanaman Pangan Tahun 2020. Kementerian Pertanian Direktorat Jenderal Tanaman Pangan.

Kasim, A., & Kadir, S. (2014). Potensi produksi varietas unggul baru kacang tanah pada wilayah pengembangan di Kabupaten Nabire. Prosiding Seminar Hasil Penelitian Tanaman Aneka Kacang dan Umbi, 663-667.

Kasim, N. N., Damayanti, T. A., Hidayat, S. H., & Santoso, S. (2022). Deteksi dan distribusi virus pada kacang tanah (Arachis hypogaea L.) di Sulawesi Selatan. Buletin Palawija, 20(2), 95-102.

Kone, N., Asare-Bediako, E., Silue, S., Kone, D., Koita, O., Menzel, W., & Winter, S. (2017). Influence of planting date on incidence and severity of viral disease on cucurbits under field condition. Annals of Agricultural Sciences, 62(1), 99–104.

Kulsum, U., Hartono, S., Sulandari, S., Sumowiyarjo, S. (2016). Identifikasi Molekuler Cowpea mild mottle virus on Soybean in Java. Jurnal Fitopatologi Indonesia, 13(6), 224-229.

Kusumasiwi, A. W. P., Muhartini, S., & Trisnowati, S. (2012). Pengaruh warna mulsa plastik terhadap pertumbuhan dan hasil terung (Solanum melongena L.) tumpangsari dengan kangkung darat (Ipomoea reptans Poir.). Vegetalika, 1(4), 118-127.

Kurnianingsih, L., & Damayanti, T. A. (2012). Lima ekstrak tumbuhan untuk menekan infeksi Bean common mosaic virus pada tanaman kacang panjang. Jurnal Fitopatologi Indonesia, 8(6), 155–160.

Lassiter, B. R., Jordan, D. L., Wilkerson, G. G., Shew, B. B., & Brandenburg, R. L. (2016). Influence of planting pattern on pest management in Virginia market type peanut (Arachis hypogaea L.). Peanut Science, 43(1), 59–66.

Lenardon, S., de Breuil, S., Oddino, C., Kearney, M., & Marinelli, A. (2002). Transmisión del Cucumber mosaic virus (CMV) en semillas de maní. XI Jornadas Fitosanitarias Argentinas, Río Cuarto, Córdoba, Argentina: 74.

Li, F., & Han, Z. (2004). Mutations in acetylcholinesterase associated with insecticide resistance in the cotton aphid. Aphis gossypii Glover. Insect Biochemistry and Molecular Biology, 34(4), 397–405.

Mabele, A.S., & Wotia, F. (2021). First report of Peanut stripe virus infecting groundnut (Arachis hypogaea L.) in Western Kenya. Biochemistry and Molecular Biology, 6(1), 11-18.

Mandal, B., Pappu, H. R., & Culbreath, A. K. (2001). Factors affecting mechanical transmission of Tomato spotted wilt virus to Peanut (Arachis hypogaea). Plant Disease, 85(12), 1259–1263.

Mandal, B., Jain, R. K., Krishnareddy, M., Krishna Kumar, N. K., Ravi, K. S., & Pappu, H. R. (2012). Emerging Problems of Tospoviruses (Bunyaviridae) and their Management in the Indian Subcontinent. Plant Disease, 96(4), 468–479.

Melinda, Damayanti, T.A., & Hidayat, S.H. (2015). Identifikasi molekuler Bean common mosaic virus yang berasosiasi dengan penyakit mosaik kuning kacang panjang. Journal of Tropical Plant Pests and Diseases, 15(2), 132-140.

Morales, F.J., & Castaño, M. (1987). Seed transmission characteristics of selected Bean common mosaic virus strains in differential bean cultivars. Plant Disease, 71(1), 51–53.

Najar A., Kumari, S., Attar, N., & Lababidi, S. (2011). First report of Chickpea chlorotic stunt virus infecting legume crops in Tunisia. Plant Disease, 95 (10), 1321-1321.

Njoki, L., Okoth, S., Wachira, P., Ouko, A., Mwololo, J., Rizzu, M., Oufensou, S., & Amakhobe, T. (2023). Evaluation of Agronomic Characteristics, Disease Incidence, Yield Performance, and Aflatoxin Accumulation among Six Peanut Varieties (Arachis hypogea L.) Grown in Kenya. Toxins, 15(2), 111.

Nurbaiti, F., Haryono, G., & Suprapto, A. (2017). Pengaruh pemberian mulsa dan jarak tanam pada hasil tanaman kedelai (Glycine max, L. Merrill.) var. Grobogan. VIGOR: Jurnal Ilmu Pertanian Tropika dan Subtropika, 2(2), 41-47.

Olubayo, F., Nderitu, J., Kibaru, A., Njeru, R., Damarisyobera, & Kasina, M., (2009). Utilization of border crops to manage aphid colonization and PVY incidence on potato (Solanum tuberosum L.). International Journal of Sustainable Crop Production, 4(5), 19–23.

Palanga, E., Filloux, D., Martin, D. P., Fernandez, E., Gargani, D., Ferdinand, R., Zabré, J., Bouda, Z., Neya, J. B., Sawadogo, M., Traore, O., Peterschmitt, M., & Roumagnac, P. (2016). Metagenomic-based screening and molecular characterization of Cowpea-infecting viruses in Burkina Faso. PLoS One, 11(10), e0165188.

Park, C.Y., Lee, M. A., Park, E. H., Baek, D. S., Lee, S. H., & Kim, J. S. (2015). First report of Cucumber mosaic virus infection of peanut (Arachis hypogaea) in South Korea. Plant Disease, 99(5), 733–733.

Paturohman, E., & Sumarno. (2014). Peningkatan Produktivitas Kacang Tanah Melalui Penerapan Komponen Teknologi Kunci. Iptek Tanaman Pangan, 9(2), 97-107.

Peters, D. (2003). Tospoviruses. In G. Loebenstein & G. Thottappilly (Eds.), Virus and Virus-like Diseases of Major Crops in Developing Countries (pp. 719–742). Springer Netherlands.

Putra, N. S. S. (2014). Efikasi Beberapa Ekstrak Tanaman untuk Mengendalikan Bean common mosaic virus pada Kacang Panjang di Lapangan [Undergraduate Theses, IPB University].

Putri, M.M., & Damayanti, T.A. (2020). Utilization of Plant Extract to Suppress Squash mosaic virus Infection on Ridged Gourd (Luffa acutangula [L.Roxb]). Journal of Tropical Plant Pests and Diseases, 20(2), 108-115.

Rodamilans, B., Valli, A., Mingot, A., San León, D., Baulcombe, D., López-Moya, J. J., & García, J. A. (2015). RNA Polymerase Slippage as a Mechanism for the Production of Frameshift Gene Products in Plant Viruses of the Potyviridae Family. Journal of Virology, 89(13), 6965–6967.

Salati, R., Nahkla, M. K., Rojas, M. R., Guzman, P., Jaquez, J., Maxwell, D. P., & Gilbertson, R. L. (2002). Tomato yellow leaf curl virus in the Dominican Republic: characterization of an infectious clone, virus monitoring in whiteflies, and identification of reservoir hosts. Phytopathology, 92(5), 487-496.

Saleh, N. (2007). Sistem Produksi Kacang-kacangan untuk Menghasilkan Benih Bebas Virus. Iptek Tanaman Pangan, 2(1), 66-78.

Saleh, N., & Baliadi, Y. (2015). Penyakit virus pada kacang tanah dan upaya pengendaliannya. Balai Penelitian Tanaman Aneka Kacang dan Umbi.

Sastry, K. S. (2013). Seed-borne plant virus diseases. Springer India.

Sastry, K. S., & Zitter, T. A. (2014). Plant Virus and Viroid Diseases in the Tropics: Volume 2: Epidemiology and Management. Springer Netherlands.

Sumarno, Adie, M.M., Saleh, N., & Adisarwanto, T. (2000). Penerapan metodologi penelitian adaptif budidaya kacang tanah di lahan petani. Penelitian Pertanian, 19(2), 51-58.

Sun, T., Zhang, Z., Ning, T., Mi, Q., Zhang, X., Zhang, S., & Liu, Z. (2015). Colored polyethylene film mulches on weed control, soil conditions and groundnut yield. Plant, Soil and Environment, 61(2), 79-85.

Sutic, D. D., Ford, R. E., & Tosic, M. T. (1999). Handbook of Plant Virus Diseases. CRC Press.

Torres, J. B., & Bueno, A. D. F. (2018). Conservation biological control using selective insecticides-a valuable tool for IPM. Biological Control, 126, 53–64.

Udayashankar, A. C., Nayaka, S. C., Kumar, H. B., Mortensen, C. N., Shetty, H. S., & Prakash, H. S. (2010). Establishing inoculum threshold levels for Bean common mosaic virus strain Blackeye cowpea mosaic infection in cowpea seed. African Journal of Biotechnology, 9(53), 8958–8969.

Valli, A., García, J. A., & López-Moya Gómez, J. J. (2021). Potyviruses (Potyviridae). In Encyclopedia of Virology (pp. 631–641). Elsevier.

Veniari, N. K. (2016). Peranan Gulma sebagai Sumber Inokulum Bean Common Mosaic Virus (BCMV) pada Kacang Panjang [Thesis, Universitas Udayana].

Verma, H. N., Baranwal, V. K., & Srivastava, S. (1998). Alternatives Strategies for Engineering Virus Resistance In Plants, In: A. Hadidi, R. K. Khetarpal, H. Kuganezawa, (eds), Plant Viruses Diseases Control. St. Paul, US: APS Press.

Wamonje, F. O., Tungadi, T. D., Murphy, A. M., Pate, A. E., Woodcock, C., Caulfield, J. C., Mutuku, J. M., Cunniffe, N. J., Bruce, T. J. A., Gilligan, C. A., Pickett, J. A., & Carr, J. P. (2020). Three aphid-transmitted viruses encourage vector migration from infected common bean (Phaseolus vulgaris) plants through a combination of volatile and surface cues. Frontiers in Plant Science, 11, 613772.

Waweru, B. W., Rukundo, P., Kilalo, D.C, Mioano, D. W, & Kimenju, J. W. (2021). Effect of border crops and intercropping on aphid infestation and the associated viral diseases in hot pepper (Capsicum sp.). Crop Protection, 145, 105623.

Wink, M. (2015). Modes of Action of Herbal Medicines and Plant Secondary Metabolites. Medicines, 2(3), 251–286.

Wisler, G. C., & Norris, R. F. (2005). Interactions between weeds and cultivated plants as related to management of plant pathogens. Weed Science, 53(6), 914–917.

Wylie, S. J., Adams, M., Chalam, C., Kreuze, J., López-Moya, J. J., Ohshima, K., Praveen, S., Rabenstein, F., Stenger, D., Wang, A., Zerbini, F. M., & ICTV Report Consortium. (2017). ICTV Virus Taxonomy Profile: Potyviridae. Journal of General Virology, 98(3), 352–354.

Xu, Z., & Barnett, O.W. (1984). Identification of a Cucumber mosaic virus strain from naturally infected peanuts in China. Plant Disease, 68(5), 386-389.

Zeller, W. (2006). Status on induced resistance against plant bacterial diseases. Fitosanidad, 10(2), 99-103.

Zhang, X. M., Lövei, G. L., Ferrante, M., Yang, N. W., & Wan, F. H. (2020). The potential of trap and barrier cropping to decrease densities of the whitefly Bemisia tabaci MED on cotton in China. Pest Management Science, 76(1), 366-374.

Zulfi, F.Z., Damayanti, T.A., & Giyanto. (2024). Management effort of mottle virus by leaf extract of Moringa (Moringa oleifera Lam.) and Bougainvillea (Bougainvillea spectabilis Willd.) on peanut. Agrovigor, 17(1).


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