Effect of Inorganic Fertilizer and VP3 Biofertilizer Applications in Legume on the Population of Indigenous Bacteria

Novi Arfarita, Tsuyoshi Imai, Cahyo Prayogo

Abstract


This current study is to examine the effects of inorganic fertilizers which are allegedly able to reduce the population of indigenous bacteria and the application of bacteria from VP3 biofertilizer on three test plants (legumes). In all treatments, the addition of inorganic fertilizer at a dose of 50% and 75% could significantly reduce population of soil bacteria on the 10th and 49th day observations. This also shows that the higher dose of NPK fertilizer also affects the bacteria from VP3 biofertilizer. However, treatment with 25–100% NPK fertilizers caused the decreasing of soil bacteria since the day of planting. In bean and long bean plants, the highest yields were shown at the combination of compost, VP3 biofertilizer and the addition of 75% NPK. Meanwhile, for mung bean, the highest yields were produced from the combination treatment of compost, VP3 and 50% and 75% NPK biofertilizers. However, the treatment of VP3 biological fertilizer with compost without the addition of NPK fertilizer on 3 legumes was able to give higher yields than the treatment of single NPK fertilizer.


Keywords


Bacterial population; Biofertilizer VP3; Combination of fertilizers; Legumes; Yield

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References


Afriliyanti, R., Yusnaini, S., Karyanto, A., Hapsoro, D., Niswati, A., & Utomo, M. (2021). Effect of long-term tillage and nitrogen fertilization residue on soil biochemical properties and cowpea yield. Journal Tropical Soils, 26(3), 141-147. DOI

Aguilar-Paredes A, Valdés G, Araneda N, Valdebenito E, Hansen F, & Nuti M. (2023). Microbial Community in the Composting Process and Its Positive Impact on the Soil Biota in Sustainable Agriculture. Agronomy, 13(2), 542. DOI

Ahmad, F., Ahmad, I., Aqil, F., Wani, A.A., & Sousche, YS. (2006). Plant growth promoting potential of free-living diazotrophs and other rhizobacteria isolated from Northern Indian soil. Biotechnology Journal, 1(10), 1112–1123. DOI

Anwar, M., Patra, D.D., Chand, S., Alpesh, K., Naqvi, A.A., & Khanuja, S.P.S. (2005) Effect of Organic Manures and Inorganic Fertilizer on Growth, Herb and Oil Yield, Nutrient Accumulation, and Oil Quality of French Basil. Communications in Soil Science and Plant Analysis, 36, 1737–1746. DOI

Arfarita, N., Hidayati, N., Rosyidah, A., Machfudz, M., & Higuchi, T. (2016). Exploration of indigenous soil bacteria producing-exopolysaccharides for stabilizing of aggregates land potential as biofertilizer. Journal of Degraded and Mining Lands Management, 4(1), 697-702. DOI

Arfarita, N., Lestari, M.W., Murwani, I., & Higuchi, T. (2017). Isolation of indigenous bacteria of phosphate solubilizing from green bean rhizospheres. Journal of Degraded and Mining Lands Management, 4(3), 845-851. DOI

Anatalia, Harsono, P., Yunindanova, M.B., & Purnomo, D. (2021). Effect of NPK Fertilizer and Foliar Fertilizer on Chili Growth and Yield Rahma. Agrotechnology Research Journal, 6(2), 73–79. website

Birkhofer, K., Bezemer, T.M., Bloem, J., Bonkowski, M., Christensen, S., Dubois, D., Ekelund, F., Fliesbach, A., Gunst, L., & Hedlund, K. (2008). Long-term organic farming fosters below and aboveground biota: Implications for soil quality, biological control and productivity. Soil Biology and Biochemistry, 40(9), 2297–2308. DOI

Chen, L., Wang, X., Zhou, W., Guo, S., Zhu, R., Qin, Y., & Sun, J. (2021) Responses of crop yields, soil enzymatic activities, and microbial communities to different long-term organic materials applied with chemical fertilizer in purple soil. European Journal of Soil Biology, 105, 103319. DOI

Cole. J.C., Smith, M.W., Penn, C.J., Cheary, B.S., & Conaghan, K.J. (2016). Nitrogen, phosphorus, calcium, and magnesium applied individually or as a slow release or controlled release fertilizer increase growth and yield and affect macronutrient and micronutrient concentration and content of field-grown tomato plants. Scientia Horticulturae, 211, 420–430. DOI

Dubey, A.K, Singh, D., Rajput, P.S., Kumar, Y., Verma, A.K., & Chandraker, S.K. (2017). Effect of NPK on plant growth, yield and quality of Capsicum (Capsicum annum L.) c.v. Swarna under shade net condition. International Journal of Current Microbiology and Applied Sciences, 6(3), 1085–1091. DOI

Farmer, J., Zhang, B., Jin, X., Zhang, P., & Wang, J. (2017). Long-term effect of plastic film mulching and fertilization on bacterial communities in a brown soil revealed by high through-put sequencing. Archives of Agronomy and Soil Science, 63 (2), 230–241. DOI

Gupta, A., & Husain, N. (2014). A critical study on the use, application and effectiveness of organic and inorganic fertilizers. Journal of Industrial Pollution Control, 30(2), 191-194. website

Hartatik, AS. (2017). Effect of Liquid Formulation of Biological Fertilizer from Vermiwash Carrier on Viability of Indigenous Bacteria and It’s Test on Vima-1 Mung Bean Germination. Thesis of the Faculty of Agriculture, Malang, Indonesia, University of Islam Malang.

Hayat, R., Ali, S., Amara, U., Khalid, R., & Ahmed, I. (2010). Soil beneficial bacteria and their role in plant growth promotion: A review. Annals of Microbiology, 60, 579–598. DOI

Hidayanti, R. (2018). Effect of Application of Biological Fertilizer with Compost on Production of Green Beans (Vigna radiata L.) and Soil Bacterial Viability. Thesis of the Faculty of Agriculture, Malang, Indonesia, University of Islam Malang.

Htwe, A.Z., Moh, S.M., Soe, K.M., Moe, K., & Yamakawa, T. (2019). Effect of biofertilizer produced from Bradyrhizobium and Streptomyces griseoflavus on plant growth, nodulation, nitrogen fixtation, nutrient uptake, and seed yield of mung bean, cowpea, and soybean. Agronomy, 9 (2), 1-12. DOI

Jakubus, M., & Bakinowska, E. (2020). Varied macronutrient uptake by plants as an effect of different fertilisation schemes evaluated by PCA. Acta Agriculturae Scandinavica, Section B-Soil & Plant Science, 70 (1), 56-68. DOI

Kaur, H., Gosal, SK., & Walia, SS. (2017). Synergistic effect of organic, inorganic and biofertilizers on soil microbial activities in rhizosphere soil of green pea. Annual Research & Review in Biology, 12 (4), 1-11. DOI

Krestini, E.H., Susilawati, A., & Hermanto, C. (2020) Effect of NPK fertilizer and microbial consortium to growth and production of garlic (Allium sativum L.). BIO Web of Conferences 20, 03010. DOI

Kuzyakov, Y. (2010). Priming effects: interactions between living and dead organic matter. Soil Biology Biochemistry, 42(9), 1363-1371. DOI

Liu, B., Gumpertz, M.L., Hu, S., & Ristaino, J.B. (2007). Long-term effects of organic and synthetic soil fertility amendments on soil microbial communities and the development of southern blight. Soil Biology and Biochemistry, 39, 2302–2316. DOI

Munthali, M.G., Gachene, C.K.K., Sileshi, G.W., & Karanja, N.K. (2014). Amendment of Tephrosia improved fallows with inorganic fertilizers improves soil chemical properties, N uptake, and maize yield in Malawi. International Journal of Agronomy, 2014, 1-9. DOI

Nuzula, HW. (2018). Effect of application of biological fertilizer with compost on production of long beans (Vigna sinensis L.) and soil bacterial viability. Thesis of the Faculty of Agriculture, Malang, Indonesia, University of Islam Malang.

Okazaki, K., Oka, N., & Tanaka, F. (2016). Effects of fertilizer and organic amendments on metabolite profiles in radish, komatsuna, and mizuna. JARQ: Japan Agricultural Research Quarterly, 50 (2), 95-99. DOI

Peix, A., Rivas-Boyero, A.A., Mateos, P., Rodriguez-Barrueco, C., Martínez-Molina, E., & Velazquez, E. (2001). Growth promotion of chickpea and barley by a phosphate solubilizing strain of Mesorhizobium mediterraneum under growth chamber conditions. Soil Biology Biochemistry, 33 (1), 103–110. DOI

Ramana, V., Ramakrishna, M., Purushotham, K., & Reddy, K.B. (2010). Effect of bio-fertilizers on growth, yield and quality of French bean (Phaseolus vulgaris L.). Legume Research, 33 (3), 178-183. website

Roba, T.B. (2018). Review on: the effect of mixing organic and inorganic fertilizer on productivity and soil fertility. Open Access Library Journal, 5, 1-11. DOI

Rodriguez, A., & Frioni, L. (2003). Characterization of rhizobia causing nodules on leguminous native trees to Uruguay using the rep-PCR technique. Revista Argentina de Microbiología, 35 (4), 193–197. website

Sessitsch, A., Howieson, J., Perret, X., Antoun, H., & Martınez-Romero, E. (2002). Advances in rhizobium research. Critical Reviews in Plant Sciences, 21, 323–378. DOI

Sharma, K., & Garg, V.K. (2018). Vermicomposting: A Green Technology for Organic Waste Management. In: Singhania, R., Agarwal, R., Kumar, R., Sukumaran, R. (eds) Waste to Wealth. Energy, Environment, and Sustainability. Springer, Singapore. DOI

Siregar, R.S., Zulia, C., & Safruddin. (2018). Effect of Trichoderma sp. dose and type of manure fertilizer application on growth and yield of long beans (Vigna sinensis L.). BERNAS Agricultural Research Journal, 14 (2), 21-34. DOI

Smale, M., & Jayne, T. (2003). Maize in Eastern and Southern Africa: “Seeds” of success in retrospect. Environment and Production Technology Division EPTD Discussion Paper 97, International Food Policy Research Institute, Washington DC, United States. PDF

Souza, E. M., Chubatsu, L. S., Huergo, L. F., Monteiro, R., Camilios-Neto, D., Wassem, R., & De Oliveira Pedrosa, F. (2014). Use of nitrogen-fixing bacteria to improve agricultural productivity. BMC Proceedings, 8(S4), O23. DOI

Syafarotin, Arfarita, N., & Lestari, M.W. (2018). Effect of application of biological fertilizer with compost on production of beans (Phaseolus vulgaris L.) and soil bacterial viability. Jurnal Folium, 2 (1), 20-30. DOI

Vessey, J.K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and soil, 255, 571-586. DOI

Wang, J.C., Song, Y., Ma, T.F., Raza, W., Li, J., Howland, J.G., Huang, Q.W., & Shen, Q.R. (2017). Impacts of inorganic and organic fertilization treatments on bacterial and fungal communities in a paddy soil. Applied Soil Ecology, 112, 42–50. DOI

Wang, H., Boutton, T. W., Xu, W., Hu, G., Jiang, P., & Bai, E. (2015). Quality of fresh organic matter affects priming of soil organic matter and substrate utilization patterns of microbes. Scientific Reports, 5(1), 10102. DOI




DOI: http://doi.org/10.17503/agrivita.v41i0.3981

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