Performance of Some Thai Weed Extracts on Antioxidants and Atherosclerosis-Related Enzymes

Sunisa U-Yatung, Wanida Suebsaiprom, Tosapon Pornprom, Jamnian Chompoo


Phytochemicals are recognized as bioactive components in herbal medicines that can cure or prevent atherosclerosis through affecting the involved factors. This study used several methods to investigate the performance of aqueous extracts from some Thai weeds on antioxidants and an atherosclerosis-related enzyme inhibitor. The inhibitory effect of aqueous extracts was expressed as the percentage of inhibition at a concentration of 500 µg/mL. GS-MS analysis was used to identify isolated compounds in sample extracts. The antioxidant activities were examined using DPPH, ABTS, nitric oxide radical scavenging and oxidation of LDL. The results showed that the aqueous extract from the leaves of Bidens pilosa had greater inhibitory effects than others (71.23, 57.89 and 50.09%, respectively, except ABTS); however, B. pilosa had weaker inhibition than the positive controls. Pancreatic lipase and 15-lipoxygenase (15-LO) had inhibitory effects regarding atherosclerosis-related enzyme activity. We found that Euphorbia hirta had stronger inhibitory activity against PL and 15-LO than other extracts (30.47 and 84.66%, respectively). Moreover, E. hirta had similar activity to quercetin against 15-LO (89.25%). Our results indicated that B. pilosa was a source of bioactive compounds such as antioxidants, while E. hirta was a source of bioactive compounds against enzymes responsible for causing atherosclerosis disease.


Balogh, E., Hegedűs, A., & Stefanovits-Bányai, É. (2010). Application of and correlation among antioxidant and antiradical assays for characterizing antioxidant capacity of berries. Scientia Horticulturae, 125(3), 332-336.

Barter, P. (2005). The role of HDL-cholesterol in preventing atherosclerotic disease. European Heart Journal, Supplements 7 (Supplement F), F4-F-8. https;//

Bartolome, A.P., Villaseñor, I.M., & Yang, W.C. (2013). Bidens pilosa L. (Asteraceae): botanical properties, traditional uses, phytochemistry, and pharmacology. Evidence-Based Complementay and Alternative Medicine, Article ID 340215, 51 p.

Bhatt, K.C., Sharma, N., & Pandey, A. (2009). 'Ladakhi tea' Bidens pilosa L. (Asteraceae): a cultivated species in the cold desert of Ladakh Himalaya, India. Genetic Resources and Crop Evolution, 56(6), 879-882.

Boskou, G., Salta, F.N., Chrysostomou, S., Mylona, A. Chiou, A., & Andrikopoulos, N.A. (2006). Antioxidant capacity and phenolic profile of table olives from the Greek market. Food Chemistry, 94(4), 558-564.

Boutaghane, N., Kabouche, A., Touzani, R., Maklad, Y.A., El-Azzouny, A., Bruneau, C., & Kabouche, Z. (2011). GC/MS analysis and analgesic effect of the essential oil of Matricaria pubescens from Algeria. Natural product Communication, 6(2), 251-252. 1100600224.

Burcu, G.B., Osman, C., Ash, C., Namik, O.M., & Neşe, B.T. (2016). The protective cardiac effects of -myrcene after global cerebral ischemia/reperfusion in C57BL/J6 mouse. Acta Cirúrgica Brasileria, 31(7), 456-462.

Campiglia, E., Radicetti, E., & Robeto, M. (2012). Weed control strategies and yield response in a pepper crop (Capsicum annuum L.) mulched with hairy vetch (Vicia villosa Roth.) and oat (Avena sativa L.) residues. Crop Protection, 33(65), 65-73.

Cherubini, A., Vigna, G.B., Zuliani, G., Ruggiero, C., Senin, U., & Fellin R. (2005). Role of antioxidants in atherosclerosis: epidemiological and clinical update. Current Pharmaceutical Design, 11(16), 2017-2032.

Cheng, S.S., Wu, C.L., Chang, H.T., Kao, Y.T., & Chang, S.T. (2004). Antitermitic and antifungal activities of essential oil of Calocedrus formosana leaf and its composition. Journal of Chemical Ecology, 30(10), 1957-1967.

Chung, I.M., Rajakumar, G., Lee, J.H., Kim, S.H., & Thiruvengadam, M., 2017. Ethnopharmacological uses, phytochemistry, biological activities, and biotechnological applications of Eclipta prostrata. Applied Microbiology and Biotechnology, 101(13), 5247-5257. 00253-017-8363-3.

Ciftci, O., Ozdemir, I., Tanyildizi, S., Yildiz, S., & Oguzturk, H. (2011). Antioxidative effects of curcumin, -myrcene and 1,8-cineole against 2,3,7,8-tetrachkorodibenzo-p-dioxin-induced oxidative stress in rat liver. Toxicology and Industrial Health, 27(5), 447-453. https// 0748233710 388452.

Cook, N.C., & Samman, S. (1996). Flavonoids-chemistry, metabolism, cardioprotective effects, and dietary source. The Journal of Nutritional Biochemistry, 7(2), 66-76.

Deba, F., Xuan, T.D., Yasuda, M., & Tawata, S. (2008). Chemical composition and antioxidant, antibacterial and antifungal activities of the essential oils from Biden pilosa Linn. var. Radiata. Food Control, 19(4), 346-352.

Devasagayam, T.P.A., Kamat, J.P., Mohan, H., & Kesavan, P.C. (1996). Caffeine as an antioxidant: inhibition of lipid peroxidation induced by reactive oxygen species. Biochimica et Biophysica Acta, 1282(1), 63-70.

Devasagayam, T.P.A., Tilak, J.C., Boloor, K.K., Sane, K.S., Ghaskadbi, S.S., & Lele, R.D. (2004). Free radicals and antioxidants in human health: current status and future prospects. The Journal of the Association of Physicians of India, 52, 794-804.

Djeridane, A., Yousfi, M., Nadjemi, B., Boutassouna, D., Stocker, P., & Vidal, N. (2006). Antioxidant activity of some Algerian medicinal plants extracts containing phenolic compounds. Food Chemistry, 97(4), 654-660.

Fuhrman, B., Rosenblat, M., Hayek, T., Coleman, R., & Aviran, M. (2000). Ginger extract consumption reduces plasma cholesterol, inhibits LDL oxidation and attenuates development of atherosclerosis in atherosclerotic, apolipoprotein E-deficient mice. American Society for Nutritional Science, 130(5), 1124-1131.

Gholamhoseinian, A., Shahouzehi, B., & Sharifi-far, F. (2010). Inhibitory effect of some plant extracts on pancreatic lipase. International Journal of Pharmacology, 6(1), 18-24. 2010.18.24.

Gianessi, L.P., 2013. The increasing importance of herbicides in worldwide crop production. Pest management Science, 69(10), 1099-1105.

Govindarajan, R., Rastogi, S., Vijayakumar, M., Shirwaikar, A., Rawat, A.K.S., Mehrotra, S., & Pushpangadan, P. (2003). Studies on the antioxidant activities of Desmodium gangeticum. Biological & Pharmaceutical Bulletin, 26(10), 1424-1427.

Gyuris, A., Szlávik, L., Minárovits, J., Vasas, A., Molnár,J. & Hohmann, J. (2009). Antiviral activities of extracts of Euphorbia hirta L. against HIV-1, HIV-2 and SIVmac251. In Vivo 23(3), 129-132.

Harats, D., Shaish, A., George, J., Mulkins, M., Kurihara, H., Levkovitz, H., & Sigal E. (2000). Overexpression of 15-lipoxygenase in vascular endothelium accelerates early atherosclerosis in LDL receptor-deficient mice. Arteriosclerosis, Thrombosis, and Vascular Biology, 20(9), 2100-2105.

Hilou, A., Nacoulma, O.G., & Guiguemde, T.R., 2006. In vitro antimalarial activities of extract from Amaranthus spinosus L. and Boerhaavia erecta L. in mice. Journal of Ethnopharmacology, 103(2), 236-240.

Hsu, C.F., Peng, H., Basle, C. Travas-Sejdic, J., & Kilmartin, P.A. (2011). ABTS•+ scavenging activity of polypyrrol, polyniline and poly(3,4-ethylenedioxythiophene). polymer International, 60(1), 69-77.

Hue, S.M., Boyce, A.N., & Somasundram, C. (2012). Antioxidant activity, phenolic and flavoid contents in the leaves of different varieties of sweet potato (Ipomoes batatas). Australian Journal of Crop Science, 6(3), 375-380.

Kähkönen, M.P., Hopia, A.I., Vuorela, H.J., Rauha, J.P., Pihlaja, K., Kujala, T., & Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of Agricultural and Food Chemistry, 47(10), 3954-3962.

Khanna, V.G., & Kannabiran, K., 2008. Antimicrobial activity of saponin fractions of the leaves of Gymnema sylvestre and Eclipta prostrata. World Journal of Microbiology and Biotechnology, 24(11), 2737-2740.

Kim, H.Y. (2007). Effect of onion (Allium cepa) skin extract on pancreatic lipase and body weight- related parameters. Food Science and Biotechnology 16(3), 434-438.

Linton, M.F., Yancey, P.G., Davies, S.S., Jerome, W.G., Linton, E.F., Song, W.L., Doran, A.C., & Vickers, K.C. (2019). The role of lipid and lipoproteins in atherosclerosis.

Loh, D.S.Y., Meng, H. & Chen, Y.S. (2009). Mutagenic and antimutagenic activities of aqueous and methanol extracts of Euphorbia hirta. Journal of Ethnopharmacology 126(3), 406-414.

Lyckander, I.M., & Malterud, K.E. (1996). Lipophilic flavonoids from Orthosiphon spicatus prevent oxidative inactivation of 15-lipoxygenase. Prostaglandins, Leukotrienes and Essential Fatty Acids 54(4), 239-246.

Magalhães, L.M., Segundo, M.A., Reis, S., Lima, J.L.F., & Rangel, A.O.S.S. (2006). Automatic method for the determination of Folin-Ciocalteu reducing capacity in food products. Journal of Agricultural and Food Chemistry, 54(15), 5241-5246.

Mandal, S., & Mandal, M. (2015). Coriander (Coriandrum sativum L.) essential oil: chemistry and biological activity. Asian Pacific Journal of Tropical Biomedicine 5(6), 421-428. j.apjtb.2015.04.001.

Moure, A., Cruz, J.M., Franco, D., Domínguez, J.M., Sineiro, J., Domínguez, H., Núnez, M.J., & Parajó, J.C. (2001). Natural antioxidants from residual sources. Food Chemistry, 72(2), 145-171.

Muchuweti, M., Mupure, C., Ndhlala, A., Murenje, T., & Benhura, M.A.N. (2007). Screening of Antioxidant and redical scavenging activity of Vigna ungiculata, Biden pilosa and Cleome gynandra. American Journal of Food Technology 2(3), 161-168.

Nowak, W.N., Deng, J., Ruan, X.Z., & Xu, Q. (2017). Reactive oxygen species generation and atherosclerosis. Arteriosclerosis, Thrombosis, and Vascular Biology, 37(5), e41-e52.

Olajid, O.A., Ogunleya, B.R., & Erinle, T.O. (2004). Anti-inflammatory properties of Amaranthus spinosus. Pharmaceutical Biology, 42(7), 521-525.

Ozcan, T., Akpinar-Bayizit, A., Yilmaz-Ersan, L., & Delikanil, B. (2014). Phenolics in human health. International Journal of Chemical Engineering and Applications, 5(5), 393-396. 10.7763/IJCEA.2014.V5.416.

Paguigan, N.D., & Chichioco-Hernandez, L. (2017). 15-Lipoxygenase inhibition of selected Philippine medicinal plants. Pharmacognosy Journal, 6(1), 43-46.

Rajeh, M.A.B., Zuraini, Z., Sasidharan, S., Latha, L.Y. & Amutha, S. 2010. Assessment of Euphorbia hirta L. leaf, flower, stem and root extracts for their antibacterial and antifungal activity and brine shrimp lethality. Molecules 2010(5), 6008-6018.

Rattan, A., & Arad, Y. (1998). Temporal and kinetic determinants of the inhibition of LDL oxidation by N-acetylcystein (NAC). Astherosclerosis, 138(2), 319-327. (98)00041-0.

Rose, J.A., & Kasum, C.M. (2002). Dietary flavonoids: bioavailability, metabolic effects and safety. Annual Review of Nutrition, 22, 19-34.

Ryu, K.R., Choi, J.Y., Chung, S., & Kim D.H. (2011). Anti-scratching behavioral effect of the essential oil and phytol isolated from Artemisia princeps Pamp. in mice. Planta Medica 77(1), 22-26.

Santos, C.C.M.P., Salvadori, M.S., Mota, V.G., Costa, L.M., Almeida, A.A.C., Oliveira, G.A.L., & Costa, J.P. (2013). Antinociceptive and antioxidant activities of phytol in vivo and in vitro model. Neuroscience Journal 2013, Article ID 949452.

Serafini, M.R., Santo, R.C., Guimarães, A.G., Santos, J.P.A., Santos, A.D.C., Alves, I.A., Gelain, D.P., Nogueira, P.C.L., Quintans-Júnior, L.J., Bonjardim, L.R., & Araújo, A.A.S. (2011). Morinda citrifolia Linn leaf extract possesses antioxidant activities and reduces nociceptive behavior and leukocyte migration. Journal of Medicinal Food, 14(10), 1159-1166.

Steinbrecher, U.P., Parthasarathy, S., Leake, D.S., Witztum, J.L., & Steinberg, D. (1984). Modification of low density lipoprotein by endothelial cells involves lipid peroxidation and degradation of low density lipoproteein phospholipids. Proceeding of the National Academy of Sciences of the United States of America, 81(12), 3883-3887.

Subramanian, S.P., Bhuvaneshwari, S. & Prasath, G.S. (2011). Antidiabetic and antioxidant potentials of Euphorbia hirta leaves extract studied in streptozotocin-induced experimental diabetes in rats. General Physiology and Biophysics 30(3), 278-285. https//

Takahashi, Y., & Yoshimoto, H.Z.T. (2005). Essential roles of lipoxygenases in LDL oxidation and development of atherosclerosis. Antioxidants & Redox Signaling 7, 425-431. 10.1089/ars.2005.7.425.

Tatiya, A.U., Surana, S.J., Khope, S.D., Gokhale, S.B., & Sutar, M.P., 2007. Phytochemical investigation and immunomodulatory activity of Amaranthus spinosus Linn. Indian Journal of Pharmaceutical Education, 44(1), 337-341.

Toledo-Ibelles, P., & Mas-Oliva, J. (2018). Antioxidants in the fight against atherosclerosis: Is this a dead end? Current Atherisclerosis Reports, 20(36), 1-12.

Tunon, M.J., Garcia-Mediavilla, M.V., Sanchez-Campos, S., & Gonzalez-Gallego, J. (2009). Potential of flavonoids as anti-inflammatory agents: modulation of pro-inflammatory gene expression and signal transduction pathways. Current Drug Metabolism, 10(3), 256-271. 138920009787846369.

Vikram, P., Chiruvella, K.K., Ripain, I.H.A. & Arifullah, M. (2014). A recent review on phytochemical constituents and medicinal properties of kesum (Polygonum minus Huds.). The Asian Pacific Journal of Tropical Biomedicine, 4(6), 430-435.

Yang, H.I., Chen, S.C., Chang, N.W., Chang, J.M., Lee, M.L., Tsai, P.C., Fu, H.H., Kao, W.W., Chiang, H.C., Wang, H.H. & Hseu, Y.C. (2006). Protection from oxidative damage using Bidens pilosa extracts in normal human erythrocytes. Food and Chemical Toxicology, 44(9), 1513-1521.


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