Large-Scale Evaluation of Indonesian Elite Maize Breeding Lines for Resistance Against Bacterial Stalk Rot Caused by Dickeya zeae

Devi Mienanti, Iqbal Hidayat, Yanuar Danaatmadja, Mohammad Bahrelfi Belaffif, Budi Waluyo, Arifin Noor Sugiharto, Ajay Goel Kumar, Kuswanto Kuswanto

Abstract


Bacterial stalk rot is one of the important diseases in maize caused by Dickeya zeae. Infection of this disease can lead to a considerable amount of loss in yield, with up to 98 percent loss of yield. The use of resistant materials is the most effective approach to managing bacterial stalk rot in maize. This study evaluates a large-scale phenotypic screening of 624 maize lines against bacterial stalk rot, divided into two groups based on a heterotic pool. These lines are used in a commercial breeding program in Indonesia. This study develops a stabbing method with a large gauge hypodermic needle, allowing for scalability in delivering inoculum while performing large-scale line evaluation. The result from ANOVA reveals a significant (P<0.05) effect of lines, day after infection, and interaction between day after infection and group. Group two displays fewer resistant lines compared to group one. This work presents a method for large-scale line evaluation for resistance against bacterial stalk rot, where the information obtained can be used in industrial breeding programs for routine material screening during the development of new lines or hybrids and for genomic studies of bacterial stalk rot resistance.

Keywords


Bacterial stalk rot; Dickeya zeae; Indonesia; Maize; Resistance

Full Text:

PDF

References


Aeny, T. N., Suharjo, R., Ginting, C., Hapsoro, D., & Niswati, A. (2020). Characterization and host range assessment of Dickeya zeae associated with pineapple soft rot disease in East Lampung, Indonesia. Biodiversitas Journal of Biological Diversity, 21(2), 587-595. DOI

Ahamad, S., Kher, D., & Lal, B. (2015). Stalk rot of maize diseases in the intermediate zone of Jammu Region. International Journal of Innovative Science, Engineering & Technology, 2(12), 1024-1032. PDF

Baer, O. T. (2022). QTL Analyses for Bacterial Stalk Rot and Diplodia Ear Rot Resistance, and Grain Yield in Maize (Zea mays L.) from Segregating and Doubled Haploid Populations [Thesis]. University of the Philipphines Los Banos.

Canama, A., & Hautea, D. (2011). Molecular mapping of resistance to bacterial stalk rot (Pectobacterium chrysanthemi pv. Zeae Burk., McFad. And Dim.) in tropical white maize (Zea mays L.). The Philippine Agriculturist Scientist, 93(4), 429–438.

Caplik, D., Kusek, M., Kara, S., Seyrek, A., & Celik, Y. (2022). First report of bacterial stalk rot of maize caused by Dickeya zeae in Turkey. New Disease Reports, 45(1), e12070. DOI

Freije, A., & Wise, K. (2016). Disease of Corn Stalk Rots (Purdue University). Purdue extension. PDF

Hooda, K. S., Bagaria, P. K., Khokhar, M., Kaur, H., & Rakshit, S. (2018). Mass Screening Techniques for Resistance to Maize Diseases. ICAR-Indian Institute of Maize Research, 93pp. PDF

Jackson-Ziems, T., Rees, J., & Harveson, R. (2014). Common Stalk Rot Diseases of Corn. Papers in Plant Pathology, 532. website

Kumar, A., Hunjan, M. S., Kaur, H., Rawal, R., Kumar, A., & Singh, P. P. (2017). A review on bacterial stalk rot disease of maize caused by Dickeya zeae. Journal of Applied and Natural Science, 9(2), 1214–1225. DOI

Lenth, R. V., Buerkner, P., Giné-Vázquez, I., Herve, M., Jung, M., Love, J., Miguez, F., Riebl, H., & Singmann, H. (2022). emmeans: Estimated Marginal Means, aka Least-Squares Means (1.8.3) [Computer software]. website

Martinez-Cisneros, B. A., Juarez-Lopez, G., Valencia-Torres, N., Duran-Peralta, E., & Mezzalama, M. (2014). First Report of Bacterial Stalk Rot of Maize Caused by Dickeya zeae in Mexico. Plant disease, 98(9), 1267–1267. DOI

Muis, A., Nonci, N., Efendy, R., & Azrai, M. (2022). The response of some genotypes of maize to downy mildew, maydis leaf blight, leaf rust, and stalk rot. IOP Conference Series: Earth and Environmental Science, 1107(1), 012004. DOI

Myung, I.-S., Jeong, I. H., Moon, S. Y., Kim, W. G., Lee, S. w., Lee, Y. H., Lee, Y.-K., Shim, H. S., & Ra, D. S. (2010). First report of bacterial stalk rot of sweet corn caused by Dickeya zeae in Korea. New Disease Reports, 22(1), 15–15. DOI

Osdaghi, E. (2022). Dickeya zeae (bacterial stalk rot of maize). CABI Compendium, CABI Compendium, 21944. DOI

R Core Team. (2021). R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing. website

Shekhar, M., & Kumar, S. (Eds.). (2012). Inoculation Methods and Disease Rating Scales for Maize Diseases (2nd ed.). Directorate of Maize Research. PDF

Singh, P., & Singh, Y. (2016). Evaluation of inoculation methods and standardization of Erwinia chrysanthemi inoculum concentration for germplasm screening against stalk rot in sorghum. Journal of Pure and Applied Microbiology, 10(4), 2747–2752. DOI

Singh, R. P., Chitara, M. K., & Chauhan, S. (2020). Bacterial stalk rot of Maize and their Management . Indian Farmers’ Digest, 53(010), 12–13.

Suriani, Patandjengi, B., Junaid, M., & Muis, A. (2021). The presence of bacterial stalk rot disease on corn in Indonesia: A review. IOP Conference Series: Earth and Environmental Science, 911(1), 012058. DOI

Suriani, S., Patandjengi, B., Muis, A., Junaid, M., Mirsam, H., Azrai, M., Efendi, R., & Sebayang, A. (2023). New corn resistant lines to stalk rot disease (Dickeya zeae) in Indonesia. Biodiversitas Journal of Biological Diversity, 24(6), Article 6. DOI

van der Wolf, J. M., Acuña, I., De Boer, S. H., Brurberg, M. B., Cahill, G., Charkowski, A. O., Coutinho, T., Davey, T., Dees, M. W., Degefu, Y., Dupuis, B., Elphinstone, J. G., Fan, J., Fazelisanagri, E., Fleming, T., Gerayeli, N., Gorshkov, V., Helias, V., le Hingrat, Y., … Yedidia, I. (2021). Diseases Caused by Pectobacterium and Dickeya Species Around the World. In F. Van Gijsegem, J. M. van der Wolf, & I. K. Toth (Eds.), Plant Diseases Caused by Dickeya and Pectobacterium Species (pp. 215–261). Springer International Publishing. DOI

Zhu, M., Tong, L., Xu, M., & Zhong, T. (2021). Genetic dissection of maize disease resistance and its applications in molecular breeding. Molecular Breeding, 41(5), 32. DOI




DOI: http://doi.org/10.17503/agrivita.v46i1.4350

Copyright (c) 2024 The Author(s)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.