Antioxidant Potential, Phenolic Content, and Nitrate/Nitrite Content in Various Lettuce Varieties

Jarongsak Pumnuan, Somsak Kramchote, Kritima Sarapothong, Thanaporn Doungnapa, Nattakarn Pisutpiboonwong, Patcharaporn Suwor

Abstract


Ten lettuce varieties are analyzed for antioxidant activity, total phenolic, nitrate, and nitrite contents, and the effects of harvest maturity and cold storage in selected varieties are determined. Antioxidant activity and total phenolic content (TPC) are the highest in Red Oak, Red Coral, Red Rapids, and Grand Rapids; intermediate in Green Oak, Green Cos, and Frillice Iceberg; and the lowest in Butterhead, Mini Green Cos, and Head lettuce. Nitrate content is the highest in Red Coral, while the other 9 varieties have comparably lower contents. Nitrite content is inadequate and does not differ with variety. Harvest maturity of 45 days after transplanting (DAT) produced the highest antioxidant activity and TPC, much higher in Red Bowl and Red Butterhead varieties than in Mini Green Cos, Butterhead, Frillice Iceberg, and Green Big Bowl varieties. Storage at 8oC for 21 days has no remarkable effects on antioxidant activity, TPC, nitrate, and nitrite contents. Stored Red Bowl lettuce has higher antioxidant activity and TPC than Butter-head and Green Big Bowl varieties. Nitrate content decreases at the end of storage, while nitrite content is below 1 mg/kg FW during the entire storage period, regardless of variety.


Keywords


DPPH free radical scavenging; Human health; Lactuca sativa L.; Nitrosamines; Nutrition

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References


Adegbaju, O. D., Otunola, G. A., & Afolayan, A.J. (2020). Effects of growth stage and seasons on the phytochemical content and antioxidant activities of crude extracts of Celosia argentea L. Heliyon, 6(6), e040862. DOI

Al-Weshahy, A., El-Nokety, M., Bakhete, M., & Rao, V. (2013). Effect of storage on antioxidant activity of freezedried potato peels. Food Research International, 50(2), 507–512. DOI

AOAC (Association of Official Analytical Chemists). (1995). AOAC official methods of analysis, 16th Edition (pp. 938–940). Virginia, USA.

Assefa, A. D., Hur, O. S., Hahn, B. S., Kim, B., Ro, N. Y., & Rhee, J.H. (2021). Nutritional metabolites of red pigmented lettuce (Lactuca sativa) germplasm and correlations with selected phenotypic characters. Foods, 10(10), 2504. DOI

Barrett, D. M., Beaulieu, J. C., & Shewfelt, R. (2010). Color, flavor, texture, and nutritional quality of fresh-cut fruits and vegetables: Desirable levels, instrumental and sensory measurement, and the effects of processing. Critical Reviews in Food Science and Nutrition, 50(5), 369–389. DOI

Blainski, A., Lopes, G. C., & De Mello, J. C. P. (2013). Application and analysis of the Folin Ciocalteu method for the determination of the total phenolic content from Limonium brasiliense L. Molecules, 18(6), 6852−6865. DOI

Chung, J. C., Chou, S. S., & Hwang, D. F. (2004). Changes in nitrate and nitrite content of four vegetables during storage at refrigerated and ambient temperature. Food Additives & Contaminants, 21(4), 317–322. DOI

Chung, W. Q., Ahmad, S. H., Zamri, M. Z., & Rosenani, A. B. (2013). Nitrate and nitrite contents and postharvest quality of choy sum (Brassica rapa chinensis Group) during storage. Acta Horticulturae, 1012, 315–320. DOI

Deng, G. F., Lin, X., Xu, X. R., Gao, L. L., Xie, J. F., & Li, H. B. (2013). Antioxidant capacities and total phenolic contents of 56 vegetables. Journal of Functional Foods, 5(1), 260–266. DOI

Flávia, M., Mariana da Costa, C., Aparecido, R. D., & Maria, S. C. (2015). Analytical methods applied for the determination of phenolic compounds in lettuce and their antioxidant activity. Academia Journal of Agricultural Research, 3(8), 116–121. website

Galani, J. H. Y., Patel, J. S., Patel, N. J., & Talati, J. G. (2017). Storage of fruits and vegetables in refrigerator increases their phenolic acids but decreases the total phenolics, anthocyanins and vitamin C with subsequent loss of their antioxidant capacity. Antioxidants, 6(3), 59. DOI

Gan, Y. Z., & Azrina, A. (2016). Antioxidant properties of selected varieties of lettuce (Lactuca sativa L.) commercially available in Malaysia. International Food Research Journal, 23(6), 2357–2362. PDF

Heimler, D., Isolani, L., Vignolini, P., Tombelli, S., & Romani, A. (2007). Polyphenol content and antioxidative activity in some species of freshly consumed salads. Journal of Agricultural and Food Chemistry, 55(5), 1724–1729. DOI

Hmelak Gorenjak, A., & Cencič, A. (2013). Nitrate in vegetables and their impact on human health. A review. Acta Alimentaria, 42(2), 158–172. DOI

Horax, R., Hettiarachchy, N., & Islam, S. (2005). Total phenolic contents and phenolic acid constituents in 4 varieties of bitter melons (Momordica charantia) and antioxidant activities of their extracts. Journal of Food Science, 70(4), C275–C280. DOI

Li, X., Wu, X., & Huang, L. (2009). Correlation between antioxidant activities and phenolic contents of Radix Angelicae Sinensis (Danggui). Molecules, 14(2), 5349–5361. DOI

Lidder, S., & Webb, A. J. (2013). Vascular effects of dietary nitrate (as found in green leafy vegetables and beetroot) via the nitrate–nitrite–nitric oxide pathway. British Journal of Clinical Pharmacology, 75(3), 677–696 DOI

Nie, Z., Wan, C., Chen, C., & Chen, J. (2019). Comprehensive Evaluation of the Postharvest Antioxidant Capacity of Majiayou Pomelo Harvested at Different Maturities Based on PCA. Antioxidants, 8(5), 136. DOI

Reinik, M., Tamme, T., & Roasto, M. (2008). Naturally Occurring Nitrates and Nitrites in Foods. In J. Gilbert & H. Z. Şenyuva (Eds.), Bioactive Compounds in Foods (1st ed., pp. 225–253). Wiley. DOI

Rumainum, I. M., Worarad, K., Srilaong, V., & Yamane, K. (2018). Fruit quality and antioxidant capacity of six Thai mango cultivars. Agriculture and Natural Resources, 52(2), 208–214. DOI

Santamaria, P., Gonnella, M., Elia, A., Parente, A., & Serio, F. (2001). Ways of reducing rocket salad nitrate content. Acta Horticulturae, 548, 529–537. DOI

Sen, S., & Chakraborty, R. (2011). The Role of Antioxidants in Human Health. In S. Andreescu & M. Hepel (Eds.), ACS Symposium Series (Vol. 1083, pp. 1–37). American Chemical Society. DOI

Serrano, M., Díaz-Mula, H. M., & Valero, D. (2011). Antioxidant compounds in fruits and vegetables and changes during postharvest storage and processing. Stewart Postharvest Review, 7(1), 1–10. DOI

Talubnak, C., Parinthawong, N., & Jaenaksorn, T. (2017). Phytoalexin production of lettuce (Lactuca sativa L.) grown in hydroponics and its invitro inhibitory effect on plant pathogenic fungi. Songklanakarin Journal of Science and Technology, 39(5), 633–640. DOI

Veljković, B., Jakovljević, V., Stanković, M., & Dajić-Stevanović, Z. (2019). Phytochemical and antioxidant properties of fresh fruits and some traditional products of wild grown raspberry (Rubus idaeus L.). Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 47(3), 565–573. DOI

Wu, S. B., Meyer, R. S., Whitaker, B. D., Litt, A., & Kennelly, E. J. (2013). A new liquid chromatography-mass spectrometry-based strategy to integrate chemistry, morphology, and evolution of eggplant (Solanum) species. Journal of Chromatography A, 1314, 154–172. DOI

Yaneva, I., Mäck, G., Vunkova-Radeva, R., & Tischner, R. (1996). Changes in nitrate reductase activity and the protective effect of molybdenum during cold stress in winter wheat grown on acid soil. Journal of Plant Physiology, 149(1-2), 211–216. DOI

Yang, H. Y., Inagaki, T., Ma, T., & Tsuchikawa, S. (2017). High-resolution and non-destructive evaluation of the spatial distribution of nitrate and its dynamics in spinach (Spinacia oleracea L.) leaves by near-infrared hyperspectral imaging. Frontiers in Plant Science, 8, 1937. DOI

Yingngam, B., Monschein, M., & Brantner, A. (2014). Ultrasound-assisted extraction of phenolic compounds from Cratoxylum formosum ssp. Formosum leaves using central composite design and evaluation of its protective ability against H2O2-induced cell death. Asian Pacific Journal of Tropical Medicine, 7(Supplement 1), S497–S505. DOI

Yosefi, Z., Tabaraki, R., Asadi Gharneh, H. A., & Mehrabi, A. A. (2010). Variation in antioxidant activity, total phenolics, and nitrate in spinach. International Journal of Vegetable Science, 16(3), 233–242. DOI

Zapata-Vahos, I. C., Rojas-Rodas, F., David, D., Gutiérrez-Monsalve, J.A., & Castro-Restrepo, D. (2020). Comparison of antioxidant contents of green and red leaf lettuce cultivated in hydroponic systems in greenhouses and conventional soil cultivation. Revista Facultad Nacional de Agronomía Medellín, 73(1), 9077–9088. DOI




DOI: http://doi.org/10.17503/agrivita.v46i1.4269

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