Email this article (Login required)
Email the author (Login required) 
In Vitro Induction and Identification of Polyploid Amorphophallus muelleri Blume Plants by Colchicine Treatment
Abstract
This research aimed to induce in vitro and identify polyploidy plantlets of Porang (Amorphophallus muelleri Blume) after colchicine treatment. In vitro shoot base explants of porang were cultured on MS medium containing different colchicine concentrations of 30, 60, 90, and 120 mg/l for four weeks while the control was medium without colchicine. After four weeks, all treated and untreated explants were multiplied and regenerated into plantlets. Flow cytometry and chromosome count were employed to determine the plantlet’s ploidy level and chromosome number, respectively. The result showed that colchicine inhibited explant growth, shoot formation, and plantlet regeneration. Increasing colchicine concentration reduces the capacity of explant in shoot formation, the number of shoots and plantlet regeneration. Of 19 plantlets regenerated from colchicine-treated explants, nine plantlets were mixoploid. Percentage of mixoploid plantlet regenerated from treated explant with 30 mg/l colchicine was higher than 60 mg/l colchicine. Mixoploid plants are mixed diploid, tetraploid, and octoploid cells. Chromosome count confirmed that the diploid cell of A. muelleri has 26 chromosomes and a colchicine-induced triploid and tetraploid cell has 39 and 54 chromosomes, respectively.
Keywords
Full Text:
PDFReferences
Abu-Qaoud, H., & Shtaya, M. J. Y. (2014). The effect of colchicine on adventitious shoot regeneration from cultured leaf explants of Petunia hybrida. British Biotechnology Journal, 4(5), 531-540. website
Anbarasan, K., Rajendran, R., Sivalingam, D., & Chidambaram, A. C. (2014). Studies on the effect of EMS and colchicine in M1 generation of sesame (Sesamum indicum L.) var. TMV3. International Letters of Natural Sciences, 16, 209-214. DOI
Ascough, G. D., van Staden, J., & Erwin, J. E. (2008). Effectiveness of colchicine andoryzalin at inducing polyploidy in Watsonia lepida N.E. Brown. HortScience, 43(7), 2248-2251. DOI
Bakry, F., de la Reberdiere, N. P., Pichot, S., & Jenny, C. (2007). In liquid medium colchicine treatment induces non chimerical doubled-diploids in a wide range of mono- and interspecific diploid banana clones. Fruits, 62(1), 3-12. DOI
Bennici, A., Schiff, S., & Mori, B. (2006). Morphogenic effect of colchicine in Cichorium intybus L. root explants cultured in vitro. Caryologia, 59(3), 284-290. DOI
Berkov, S., & Philipov, S. (2002). Alkaloid production in diploid and autotetraploid plants of Datura stramonium. Pharmaceutical Biology, 40(8),617–621. DOI
Bhusare, B. P., John, C. K., Bhatt, V. P., & Nikam, T. D. (2021). Colchicine induces tetraploids in vitro cultures of Digitalis lanata Ehrh: Enhanced production of biomass and cardiac glycoside. Industrial Crops and Products, 174, 114167. DOI
Blasco, M., Badenes, M. L., & del Mar Naval, M. (2015). Colchicine-induced polyploidy in loquat (Eriobotrya japonica (Thunb.) Lindl.). Plant Cell, Tissue and Organ Culture, 120, 453-461. DOI
Diem, L. T., Phong, T. H., Tung, H. T., Khai, H. D., Anh, T. T. L., Mai, N. T. N., … Nhut, D. T. (2022). Tetraploid induction through somatic embryogenesis in Panax vietnamensis Ha et Grushv. by colchicine treatment. Scientia Horticulturae, 303, 111254. DOI
Ewald, D., Ulrich, K., Naujoks, G., & Schröder, M.-B. (2009). Induction of tetraploid poplar and black locust plants using colchicine: chloroplast number as an early marker for selecting polyploids in vitro. Plant Cell, Tissue and Organ Culture, 99, 353–357. DOI
Farhadi, N., Panahandeh, J., Motallebi-Azar, A., & Mokhtarzadeh, S. (2022). Production of autotetraploid plants by in vitro chromosome engineering in Allium hirtifolium. Horticultural Plant Journal, 2022, 1-13. DOI
Gao, S. L., Chen, B. J., & Zhu, D. N. (2002). In vitro production and identification of autotetraploids of Scuttellaria haicalensis. Plant Cell, Tissue and Organ Culture, 70, 289–293. DOI
Gao, S. L., Zhu, D. N., Cai, Z. H., & Xu, D. R. (1996). Autotetraploid plants from colchicine-treated bud culture of Salvia miltiorrhiza Bge. Plant Cell, Tissue and Organ Culture, 47, 73–77. DOI
Huang, H.-P., Gao, S.-L., Chen, L.-L., & Wei, K.-H. (2010). In vitro tetraploid induction and generation of tetraploids from mixoploids in Dioscorea zingiberensis. Pharmacognosy Magazine, 6(21), 51-56. DOI
Kim, Y.-S., Hahn, E.-J., Murthy, H. N., & Paek, K.-Y. (2004). Effect of polyploidy induction on biomass and ginsenoside accumulations in adventitious roots of Ginseng. Journal of Plant Biology, 47, 356–360. DOI
Kucharska, D., Podwyszyńska, M., Trzewik, A., Marasek-Ciołakowska, A., Pluta, S., & Seliga, Ł. (2022). In vitro induction and primary evaluation of octoploid plants in saskatoon berry (Amelanchier alnifolia Nutt.). Agronomy, 12(5), 1215. DOI
Kwon, S.-J., Seo, D.-Y., Cho, G.-Y., Lee, M.-S., Moon, Y.-J., Boo, H.-O., … Kim, H.-H. (2016). Effect of colchicine on chromosome doubling in Codonopsis lanceolata. Korean Journal of Plant Resources, 29(3), 347-354. DOI
Leung, Y. Y., Hui, L. L. Y., & Kraus, V. B. (2015). Colchicine—Update on mechanism of action and therapeutic uses. Seminars in Arthritis and Rheumatism, 45(3), 341-350. DOI
Li, M., Ding, B., Huang, W., Pan, J., Ding, Z., & Jiang, F. (2018). Induction and characterisation of tetraploids from seeds of Bletilla striata (Thunb.) Reichb.f. BioMed Research International, 2018, 3246398. DOI
Liu, R., Gao, C., Jin., J., Wang, Y., Jia, X., Ma, H., … Xu, J. (2022). Induction and identification of tetraploids of pear plants (Pyrus bretschneideri and Pyrus betulaefolia). Scientia Horticulturae, 304, 111322. DOI
Liu, W.-T., Zheng, Y.-F., Song, S.-Y., Huo, B.-B., Li, D.-L., & Wang, J. (2018). In vitro induction of allohexaploid and resulting phenotypic variation in Populus. Plant Cell, Tissue and Organ Culture, 134, 183–192. DOI
Manzoor, A., Ahmad, T., Bashir, M. A., Hafiz, I. A., & Silvestri, C. (2019). Studies on colchicine induced chromosome doubling for enhancement of quality traits in ornamental plants. Plants, 8(7), 194. DOI
Mo, L., Chen, J., Lou, X., Xu, Q., Dong, R., Tong, Z., … Lin, E. (2020). Colchicine-Induced polyploidy in Rhododendron fortunei Lindl. Plants, 9, 424. DOI
Nilanthi, D., Chen, X.-L., Zhao, F.-C., Yang, Y.-S., & Wu, H. (2009). Induction of tetraploids from petiole explants through colchicine treatments in Echinacea purpurea L. Biomed Research International, 2009, 343485. DOI
Omezzine, F., Ladhari, A., Nefzi, F., Harrath, R., Aouni, M., & Haouala, R. (2012). Induction and flow cytometry identification of mixoploidy through colchicine treatment of Trigonella foenum-graecum L. African Journal of Biotechnology, 11(98), 16434-16442. Retrieved from website
Omidbaigi, R., Mirzaee, M., Hassani, M. E., & Sedghi Moghadam, M. (2010). Induction and identification of polyploidy in basil (Ocimum basilicum L.) medicinal plant by colchicine treatment. International Journal of Plant Production, 4(2), 87-98. DOI
Pliankong, P., Suksa-Ard, P., & Wannakrairoj, S. (2017). Effects of colchicine and oryzalin on polyploidy induction and production of capsaicin in Capsicum frutescens L. Thai Journal of Agricultural Science, 50(2), 108-120. Retrieved from PDF
Poerba, Y. S., Imelda, M., Wulansari, A., & Martanti, D. (2009). Induksi mutasi kultur in vitro Amorphophallus muelleri Blume dengan irradiasi gamma. Jurnal Teknologi Lingkungan, 10(3), 255-364. DOI
Saleh, N., Rahayuningsih, S. A., Radjit, B. S., Ginting, E., Harnowo, D., & Mejaya, I M. J. (2015). Tanaman porang: Pengenalan, budidaya dan pemanfaatannya. Bogor: Pusat Penelitian dan Pengembangan Tanaman Pangan, Badan Penelitian dan Pengembangan Pertanian. Retrieved from website
Samatadze, T. E., Yurkevich, O. Y., Khazieva, F. M., Basalaeva, I. V., Konyaeva, E. A., Burova, A. E., … Muravenko, O. V. (2022). Agro-Morphological and cytogenetic characterization of colchicine-induced tetraploid plants of Polemonium caeruleum L. (Polemoniaceae). Plants, 11(19), 2585. DOI
Santosa, E. (2014). Pengembangan tanaman iles-iles tumpangsari untuk kesejahteraan petani dan kemandirian industri pangan nasional. Jurnal Risalah Kebijakan Pertanian dan Lingkungan, 1(2), 73-79. Retrieved from website
Sourour, A., Ameni, B., & Mejda, C. (2014). Efficient production of tetraploid barley (Hordeum vulgare L.) by colchicine treatment of diploid barley. Journal of Experimental Biology and Agricultural Sciences, 2(1S),113-119. Retrieved from PDF
Taylor, E. W. (1965). The mechanism of colchicine inhibition of mitosis: I. Kinetics of Inhibition and the Binding of H3-Colchicine. Journal of Cell Biology, 25(1), 145–160. DOI
Tulay, E., & Unal, M. (2010). Production of colchicine induced tetraploids in Vicia villosa roth. Caryologia, 63(3), 292-303. DOI
Tuwo, M., & Indrianto, A. (2016). Improvement of orchid vanda hybrid (Vanda limbata Blume X Vanda tricolor Lindl. var. suavis) by colchicines treatment in vitro. Modern Applied Science, 10(11), 83-89. DOI
Udensi, O. U., & Ontui, V. (2013). Determination by flow cytometry polyploidy inducing-capacity of colchicine in Cajanus cajan (L.) Mill sp. Pakistan Journal of Biological Sciences, 16(13), 630-635. DOI
Väinölä, A., & Repo, T. (2001). Polyploidisation of Rhododendron cultivars in vitro and how it affects cold hardiness. Acta Horticulturae, 560, 319–322. DOI
Wahyuni, K. I., Rohmah, M. K., Ambari, Y., & Romadhon, B. K. (2020). Pemanfaatan umbi porang (Amorphophallus muelleri Bl) sebagai bahan baku keripik. Jurnal Karinov, 3(1), 1-4. Retrieved from PDF
Widoretno, W. (2016). In vitro induction and characterization of tetraploid Patchouli (Pogostemon cablin Benth.) plant. Plant Cell, Tissue, and Organ Culture, 125(2), 261-267. DOI
Wu, J., Cheng, X., Kong, B., Zhou, Q., Sang, Y., & Zhang, P. (2022). In vitro octaploid induction of Populus hopeiensis with colchicine. BMC Plant Biology, 22, 176. DOI
Xu, C.-P., Huang, Z., Liao, T., Li, Y., & Kang, X.-Y. (2016). In vitro tetraploid plants regeneration from leaf explants of multiple genotypes in Populus. Plant Cell, Tissue and Organ Culture, 125, 1–9. DOI
Ye, Y. M., Tong, J., Shi, X. P., Yuan, W., & Li, G. R. (2010). Morphological and cytological studies of diploid and colchicine-induced tetraploid lines of crape myrtle (Lagerstroemia indica L.). Scientia Horticulturae, 124(1), 95-101. DOI
Yudha, M. W. (2022). Strategi ekspor tanaman porang di provinsi Sumatera Utara melalui Balai Besar Karantina Pertanian Belawan [Master Thesis]. Medan: Universitas Medan Area. Retrieved from website
DOI: http://doi.org/10.17503/agrivita.v45i1.3992
Copyright (c) 2023 The Author(s)
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
