Mycelial Growth and Basidiocarp Production of Pleurotus Species in Substrates Supplemented with Fermented Plant Juice

Leilidyn Y. Zurbano, Diana A. Antones, Carla Mae C. Almoradie


Pleurotus species are the common mushrooms popularly cultivated in the Philippines while Fermented Plant Juice (FPJ) is a known organic concoction which can help crops to grow. This study aimed to find out the effects of FPJ on the mycelial growth, basidiocarp production and sensory attributes of different species of Pleurotus in culture media, grain spawn and fruiting substrates supplemented with varying amounts of FPJ. The experiment was laid out in complete randomized design and replicated thrice for culture media and grain spawn; and replicated ten times for the fruiting substrates. P. florida in 20 ml culture media supplemented with 2.4 ml of the 120 ml FPJ solution had the fastest mycelial growth while P. citronipleatus in grain spawn supplemented with water had the fastest mycelial growth. P. ostreatus grown in 750 g dry weight of fruiting substrates supplemented with 150 ml of FPJ had 6.83 higher yield than the control, thus higher profitability and Return on Investment (ROI). Significant differences were also observed on the sensory attributes such as taste, aroma and general acceptability. Majority of the respondents liked P. florida which is considered as very much palatable. As the concentration of FPJ become higher, the taste becomes better.


Fermented Plant Juice; Pleurotus citrinopileatus; Pleurotus djamor; Pleurotus florida; Pleurotus ostreatus

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Alananbeh, K. M., Bouqellah, N. A., & Al Kaff, N. S. (2014). Cultivation of oyster mushroom Pleurotus ostreatus on date-palm leaves mixed with other agro-wastes in Saudi Arabia. Saudi Journal of Biological Sciences, 21(6), 616-625. DOI

Chae, H.-J., & Ahn, J.-H. (2013). Optimization of rice bran and food waste compost contents in mushroom culture medium to maximize mycelial growth rate and fruit body yield of Pleurotus ostreatus. International Biodeterioration and Biodegradation, 80, 66-70. DOI

Chang, H.-Y., Jeon, S.-W., Cosadio, A. L., Icalina, I. L., Panganiban, R., Quirino, R. A., & Song, Y. (2014). Status and prospect of mushroom industry in the Philippines. JPAIR Multidisciplinary Research Journal, 16(1), 1-16. Retrieved from website

Coklar, H., & Akbulut, M. (2020). The control of Maillard reaction in white grape molasses by the method of reducing reactant concentration. Food Science and Technology, 40, 179-189. Retrieved from website

De Leon, A. M., Reyes, R. G., & dela Cruz, T. (2013). Enriched cultivation of three wild strains of Lentinus tigrinus (Bull.) Fr. using agricultural wastes. Journal of Agricultural Technology, 9(5), 1199-1214. Retrieved from PDF

Dissasa, G. (2022). Cultivation of different oyster mushroom (Pleurotus species) on coffee waste and determination of their relative biological efficiency and pectinase enzyme production, Ethiopia. International Journal of Microbiology, 2022, 1-10. DOI

Hoa, H. T., Wang, C.-L., & Wang, C.-H. (2015). The effects of different substrates on the growth, yield, and nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology, 43(4), 423–434. DOI

Kalaw, S. P., Nabor, R., Dulay, R. M. R., Undan, J. R., Undan, J. Q., De Leon, A. M., & Reyes, R. G. (2017). Mycelia and fruiting body performance of Philippine wild strains of Ganoderma lucidum and Schizophyllum commune on rice bran enriched rice straw based substrates. International Journal of Biology, Pharmacy, and Allied Sciences, 6(6), 1210-1219. Retrieved from PDF

Knop, D., Yarden, O., & Hadar, Y. (2015). The ligninolytic peroxidases in the genus Pleurotus: divergence in activities, expression, and potential applications. Applied Microbiology and Biotechnology, 99, 1025–1038. DOI

Kokkinidou, S., Peterson, D., Bloch, T., & Bronston, A. (2018). The important role of carbohydrates in the flavor, function, and formulation of oral nutritional supplements. Nutrients, 10(6), 742. DOI

Kumla, J., Suwannarach, N., Sujarit, K., Penkhrue, W., Kakumyan, P., Jatuwong, K., … Lumyong, S. (2020). Cultivation of mushrooms and their lignocellulolytic enzyme production through the utilization of agro-industrial waste. Molecules, 25(12), 2811. DOI

Lawless, H. T., & Heymann, H. (2010). Sensory evaluation of food: Principles and practices. New York: Springer. DOI

Liu, G., Zhang, Y., Gong, H., Li, S., Pan, Y., Davis, C., … Godwin, I. D. (2021). Stem vacuole-targetted sucrose isomerase enhances sugar content in sorghum. Biotechnology for Biofuels, 14, 53. DOI

Luis, J. S. (1999). Production of oyster mushroom (Pleurotus) and Shitake (Lentinula edodes) in Benguet, Philippines. Retrieved from website

Memon, A. A., Mangrio, G. S., Kaleri, A. A., Kumar, B., Khan, M., Kaleri, R. R., … Wahocho, N. A. (2017). Effect of dextrose sugar on the growth and production of oyster mushroom (Pleurotus ostreatus) through tissue culture. Journal of Basic & Applied Sciences, 13, 139-142. Retrieved from PDF

Miller, S. A., Ikeda, D. M., Weinert Jr, E., Chang K. C. S., McGinn, J. M., Keliihoomalu, C., & DuPonte, M. W. (2013). Natural farming: Fermented plant juice. Sustainable Agriculture, SA-7. Hawaii: College of Tropical Agriculture and Human Resources, University of Manoa. Retrieved from PDF

Mkhize, S. S., Cloete, J., Basson, A. K., & Zharare, G. E. (2016). Performance of Pleurotus ostreatus mushroom grown on maize stalk residues supplemented with various levels of maize flour and wheat bran. Food Science and Technology, 36(4): 598-605. DOI

Oh, M.-J., Kim, Y., Lee, S.-H., Lee, K.-W., & Park, H.-Y. (2018). Prediction of CML contents in the Maillard reaction products for casein-monosaccharides model. Food Chemistry, 267, 271-276. DOI

Ramachela, K., & Sihlangu, S. M. (2016). Effects of various hormonal treated plant substrates on development and yield of Pleurotus ostreatus. Cogent Food & Agriculture, 2(1), 1276510. DOI

Reyes, R. G., Grassel, W., & Rau, U. (2009). Coconut water as a novel culture medium for the biotechnological production of schizophyllan. Journal of Nature Studies, 7(2), 43-48. Retrieved from website

Royse, D. J. (2014). A global perspective on the high five: Agaricus, Pleurotus, Lentinula, Auricularia & Flammulina. Paper presented at Proceedings of the 8th International Conference on Mushroom Biology and Mushroom Products (ICMBMP8) 2014 November 19–22; New Delhi, India. Retrieved from PDF

Sahu, S. K., Singh, D. P., Patel, R., & Awadhiya, G. K. (2014). Screening of suitable grains substrates for Spawn development, growth and yield of Pleurotus eous. American International Journal of Research in Formal, Applied & Natural Sciences, 5(1), 86-89. Retrieved from PDF

Singh, M. P., & Singh, V. K. (2011). Yield performance and nutritional analysis of Pleurotus citrinopileatus on different agro wastes and vegetable wastes. Paper presented at Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7) 2011 October 4–7; Arcachon, France. Retrieved from website

Zawirksa-Wojtasiak, R. (2004). Optical purity of (R)-(−)-1-octen-3-ol in the aroma of various species of edible mushrooms. Food Chemistry, 86(1), 113–118. DOI

Zurbano, L. Y., Bellere, A. D., & Savilla, L. C. (2017). Mycelial growth, fruiting body production and proximate composition of Pleurotus djamor on different substrate. CLSU International Journal of Science & Technology, 2(1), 20-30. DOI

Zurbano. L. Y. (2018). Mycelial growth and fructification of earwood mushroom (Auricularia polytricha) on different substrates. Paper presented at 4th International Research Conference on Higher Education IRCHE 2017 (pp. 799–814). DOI


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