Effect of Gamma Ray Irradiation and In vitro selection on “Siem Banyuwangi” (Citrus nobilis (L)) to Huanglongbing disease

Dumaris Priskila Purba, Ali Husni, Alina Akhidaya, Mia Kosmiatin, Agus Purwito

Abstract


The siam orange (Citrus nobilis L.) holds great economic and social importance, but it is susceptible to Huanglongbing-HLB disease. HLB disease caused by Ca. Liberabacter spp which is a specific bacterium phloem vessels by callouses forming that covering plasmodesmata. This research aims to improve tolerance siam orange from Banyuwangi (SB) to HLB disease. The experiment was arrange complete randomized design (CRD) with one factor, the irradiation dose. This study consisted of two interrelated experiments, namely: Induction of embryo mutations with gamma ray irradiation, and In vitro selection of putative mutant. Embryo of SB was irradiated by gamma ray irradiation with dose 0, 45, 50, and 55 Gray. Each treatment was repeated five times with 20 embryos. After 24 weeks, In vitro shoots putative mutant were screened by HLB pathogen suspense. The observations showed that the treatment of various doses of gamma rays had an influence on embryo germination. In vitro selection results that putative mutant on dose 45, 50, and 55 showed tolerant to HLB after selected.


Keywords


Mutation Breeding; Huanglongbing; CVPD; In vitro breeding; suspension of HLB pathogen

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DOI: http://doi.org/10.17503/agrivita.v43i2.2887

Copyright (c) 2021 Dumaris Priskila Purba, Ali Husni, Alina Akhidaya, Mia Kosmiatin, Agus Purwito

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