Effectiveness of Bacteria Isolated from Peat Swamp Forests to Control Rice Dirty Panicle Fungi in Thailand

Jintana Unartngam, Thiphaphorn Naunnet, Sasawat Sangsuk, Orawan Chountragoon, Chommanat Kerdkhong, Manee Tantirungkij

Abstract


Rice dirty panicle disease is one of the most important problems in Thailand. The fungal pathogens were reported to be many species including Curvularia lunata, Bipolaris oryzae, Fusarium incarnatum, Sarocladium oryzae, Trichoconis padwickii and Cercospora oryzae. Biological control is an alternative method for controlling this disease and reducing the application of fungicides. Therefore, this study aimed to evaluate the antagonistic potential of bacteria isolated from peat swamp forests in Thailand. A total of 513 bacterial isolates were collected and screened in the laboratory using the dual culture method. The results revealed that three of the 513 bacterial strains (IBK-4, IBK-8 and IBK-5) were highly inhibitory to the fungal pathogens including C. lunata, B. oryzae and F. incarnatum. These three strains were identified as Bacillus (IBK-4 and IBK-8) and Brevibacillus (IBK-5) based on 16S rRNA sequencing. Then, these three strains were evaluated on a susceptible rice variety by inoculation with three fungal pathogens. The results indicated that Bacillus strain IBK-8 had the highest efficiency to control the disease development as observed in the disease severity and index. The results of this study indicated that bacterial strains from peat swamp forests have the potential to be antagonistic to plant pathogens.


Keywords


Bacteria; Biological control; Peat swamp forest; Plant disease; Rice dirty panicle

Full Text:

PDF

References


Adebayo, O. S., & Ekpo, E. J. A. (2004). Efficiency of fungal and bacterial biocontrol organisms for the control of Fusarium wilt of tomato. Nigerian Journal of Horticultural Science, 9(1), 63–68. DOI

Ahmed, A. I. (2017). Biological control of potato brown leaf spot disease caused by Alternaria alternata using Brevibacillus formosus strain DSM 9885 and Brevibacillus brevis strain NBRC 15304. Journal of Plant Pathology & Microbiology, 8(6), 1–8. DOI

Altschul, S. F., Madden, T. L., Schäffer, A. A., Zhang, J., Zhang, Z., Miller, W., & Lipman, D. J. (1997). Gapped BLAST and PSI-BLAST: A new generation of protein database search programs. Nucleic Acids Research, 25(17), 3389–3402. DOI

Athukorala, S. N. P., Fernando, W. G. D., & Rashid, K. Y. (2009). Identification of antifungal antibiotics of Bacillus species isolated from different microhabitats using polymerase chain reaction and MALDI-TOF mass spectrometry. Canadian Journal of Microbiology, 55(9), 1021–1032. DOI

Charoenrak, P., & Chamswarng, C. (2016). Efficacies of wettable pellet and fresh culture of Trichoderma asperellum biocontrol products in growth promoting and reducing dirty panicles of rice. Agriculture and Natural Resources, 50(4), 243–249. DOI

Chen, X. H., Scholz, R., Borriss, M., Junge, H., Mögel, G., Kunz, S., & Borriss, R. (2009). Difficidin and bacilysin produced by plant-associated Bacillus amyloliquefaciens are efficient in controlling fire blight disease. Journal of Biotechnology, 140(1–2), 38–44. DOI

Cordero, P., Príncipe, A., Jofré, E., Mori, G., & Fischer, S. (2014). Inhibition of the phytopathogenic fungus Fusarium proliferatum by volatile compounds produced by Pseudomonas. Archives of Microbiology, 196, 803–809. DOI

Dihazi, A., Jaiti, F., WafaTaktak, Kilani-Feki, O., Jaoua, S., Driouich, A., ... Serghini, M. A. (2012). Use of two bacteria for biological control of bayoud disease caused by Fusarium oxysporum in date palm (Phoenix dactylifera L) seedlings. Plant Physiology and Biochemistry, 55, 7–15. DOI

Felsenstein, J. (1985). Confidence limits on phylogenies: An approach using the bootstrap. Evolution, 39(4), 783–791. DOI

Grover, M., Nain, L., & Saxena, A. K. (2009). Comparision between Bacillus subtilis RP24 and its antibioticdefective mutants. World Journal of Microbiology and Biotechnology, 25, 1329–1335. DOI

Khalid, A., Arshad, M., & Zahir, Z. A. (2004). Screening plant growth-promoting rhizobacteria for improving growth and yield of wheat. Journal of Applied Microbiology, 96(3), 473–480. DOI

Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870–1874. DOI

Mahdiyah, D., Farida, H., Riwanto, I., Mustofa, Wahjono, H., Nugroho, T. L., & Reki, W. (2020). Screening of Indonesian peat soil bacteria producing antimicrobial compounds. Saudi Journal of Biological Sciences, 27(10), 2604-2611. DOI

Marroni, I. V, & Germani, J. C. (2011). Eficiência de rizobactérias Bacillus spp. no controle in vitro de Macrophomina phaseolina agente etiológico da podridão de tronco damamona (Ricinus communis L). Revista Brasileira de Agroecologia, 6(3), 159–167. Retrieved from PDF

McMaugh, T. (2005). Guidelines for surveillance for plant pests in Asia and the Pacific. ACIAR Monograph No. 119. Canberra, Australia. Retrieved from PDF

Nookongbut, P., Kantachote, D., Khuong, N. Q., Sukhoom, A., Tantirungkij, M., & Limtong, S. (2019). Selection of acid-resistant purple nonsulfur bacteria from peat swamp forests to apply as biofertilizers and biocontrol agents. Journal of Soil Science and Plant Nutrition, 19, 488-500. DOI

Raza, W., Ling, N., Zhang, R., Huang, Q., Xu, Y., & Shen, Q. (2017). Success evaluation of the biological control of Fusarium wilts of cucumber, banana, and tomato since 2000 and future research strategies. Critical Reviews in Biotechnology, 37(2), 202–212. DOI

Rocha, F. Y. O., de Oliveira, C. M., da Silva, P. R. A., de Melo, L. H. V., do Carmo, M. G. F., & Baldani, J. I. (2017). Taxonomical and functional characterization of Bacillus strains isolated from tomato plants and their biocontrol activity against races 1, 2 and 3 of Fusarium oxysporum f. sp. Lycopersici. Applied Soil Ecology, 120, 8–19. DOI

Saitou, N., & Nei, M. (1987). The neighbor-joining method: A new method for reconstructing phylogenetic trees. Molecular Biology and Evolution, 4(4), 406–425. DOI

Schaad, N. W., Jones, J. B., & Chun, W. (Eds.). (2001). Laboratory guide for identification of plant pathogenic bacteria (3rd ed.). The American Phytopathological Society. Retrieved from website

Vingataramin, L., & Frost, E. H. (2015). A single protocol for extraction of gDNA from bacteria and yeast. BioTechniques, 58(3), 120–125. DOI

Yanti, Y., Warnita, Reflin, & Busniah, M. (2018). Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wilt disease on chili pepper. Biodiversitas, 19(4), 1532–1538. DOI




DOI: http://doi.org/10.17503/agrivita.v43i2.2779

Copyright (c) 2021 The Author(s)

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.