Evaluation of Culture Media For In Vitro Conservation of Gladiolus Cultivars

Kurniawan Budiarto, Teresita L. Rosario

Abstract


In vitro conservation is considered to be one promising tool in preserving plant genetic resources like gladiolus when grown in the tropics.  The study was conducted at the Indonesian Ornamental Crops Research Institute (IOCRI) from February 2016 to August 2018.  A complete factorial experiment was designed to facilitate the combination of two factors, i.e. gladiolus cultivars and media compositions for in vitro conservation. The results showed that the trends of plantlet survival rate and viability in every 4 months observation of both cultivars were similar.  The highest plantlet death rate was detected during 12 – 16 months.  After 24 months storage, the differences in plantlet survival and viability were observed. Gladiolus ‘Nafa’ had higher plantlet survivals and viability than ‘Kaifa’.  In respect to nutrient and sucrose concentration, full nutrient strength preserved 5% higher plantlet survival and viability than ½ MS.  While media contained sucrose 90 g/l also supported plantlet survival and viability for more than 20% and 17%, respectively than lower sucrose media.


Keywords


Gladiolus, genotype, in vitro conservation sucrose, death rates, viability, in vitro, salt strength.

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DOI: http://doi.org/10.17503/agrivita.v0i0.2314

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