Utilization of Vermiwash for the Production of Liquid Biofertilizers and Its Effect on Viability of Inoculant Bacteria and Green Bean Germination

Novi Arfarita, Mahayu Woro Lestari, Cahyo Prayogo


Contribution of biofertilizer in Indonesia as an alternative material for improving soil fertility and crop production is still limited. This may due to several major obstacles on its utilization and effectiveness regarding to it quality in term of its microbial viability of inoculants on formulations. This study aims to determine the effect of liquid biofertilizer formulations made from vermiwash carrier material on indigenous bacterial viability and its testing on germination of green beans ‘Vima-1’. The study included: (1) optimization of pH for the growth of inoculants bacterial consortium in vermiwash formulations with various additives, (2) viability tests and (3) pathogenicity tests of biological fertilizers on green bean sprouts (vegetative phase). The results showed that the best pH for the growth of the consortium of three bacterial isolates was at 5.5. Liquid formulation with a carrier material in the form of vermiwash plus 1% PEG (VP3 treatment) supported the highest viability of the bacterial consortium during 8 weeks incubation. All treatments did not inhibit the growth of green bean sprouts and symptom of pathogenicity was absent in all treated plants. From all treatments, the tallest green bean sprout was detected at VP3 treatment.


Bacterial viability; Exopolysaccharide; Liquid formulation; Pathogenity

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Agegnehu, G., VanBeek, C., & Bird, M. I. (2014). Influence of integrated soil fertility management in wheat and tef productivity and soil chemical properties in the highland tropical environment. Journal of Soil Science and Plant Nutrition, 14(3), 532–545. DOI

Arfarita, N., Muhibuddin, A., & Imai, T. (2019). Exploration of indigenous free nitrogen-fixing bacteria from rhizosphere of Vigna radiata for agricultural land treatment. Journal of Degraded and Mining Lands Management, 6(2), 1617–1623. DOI

Arif, M., Jan, M. T., Khan, N. U., Khan, A., Khan, M. J., & Munir, I. (2010). Effect of seed priming on growth parameters of soybean. Pakistan Journal of Botany, 42(4), 2803–2812. Retrieved from PDF

Boraste, A., Vamsi, K. K., Jhadav, A., Khairnar, Y., Gupta, N., Trivedi, S., … Joshi, B. (2009). Biofertilizers: A novel tool for agriculture. International Journal of Microbiology Research, 1(2), 23–31. DOI

Dastager, S. G., Deepa, C. K., Puneet, S. C., Nautiyal, C. S., & Pandey, A. (2009). Isolation and characterization of plant growth‐promoting strain Pantoea NII‐186. From Western Ghat Forest soil, India. Letters in Applied Microbiology, 49, 20–25. DOI

Fahrurrozi, F., Muktamar, Z., Setyowati, N., Sudjatmiko, S., & Chozin, M. (2019). Comparative Effects of Soil and Foliar Applications of Tithonia-Enriched Liquid Organic Fertilizer on Yields of Sweet Corn in Closed Agriculture Production System. AGRIVITA, Journal of Agricultural Science, 41(2). DOI

Finlay, W. J. J., Logan, N. A., & Sutherland, A. D. (2000). Bacillus cereus produces most emetic toxin at lower temperatures. Letters in Applied Microbiology, 31, 385–389. DOI

Giyanto, Suhendar, A., & Rustam. (2009). Kajian pembiakan bakteri kitinolitik Pseudomonas fluorescens dan Bacillus sp. pada limbah organik dan formulasinya sebagai pestisida hayati (Biopesticide). In Prosiding Seminar Hasil-Hasil Penelitian IPB 2009 (p. 849). Bogor, ID: Institut Pertanian Bogor. Retrieved from website

Gulsar, B. J., & Rohini, I. (2006). Effect of vermiwash on nematodes prevalent in coconut based high-density multispecies cropping system. Indian Journal of Nematology, 36(2), 176–180. Retrieved from website

Haruta, S., & Kanno, N. (2015). Survivability of microbes in natural environments and their ecological impacts. Microbes and Environments, 30(2), 123–125. DOI

Husen, E. H., Simanungkalit, R. D. M., Saraswati, R., & Irawan. (2007). Characterization and quality assessment of Indonesian commercial biofertilizers. Indonesian Journal of Agricultural Science, 8(1), 31–38. DOI

Iqbal, N., Khan, N. A., Ferrante, A., Trivellini, A., Francini, A., & Khan, M. I. R. (2017). Ethylene Role in Plant Growth, Development and Senescence: Interaction with Other Phytohormones . Frontiers in Plant Science , Vol. 8, p. 475. DOI

Khairuddin, M. N., Isa, I. M., Zakaria, A. J., & Rani, A. R. A. (2018). Effect of amending organic and inorganic fertilizer on selected soil physical properties in entisols. AGRIVITA, Journal of Agricultural Science, 40(2), 242–248. DOI

Latifah, E., Dewi, H. A., Daroini, P. B., Korlina, E., Hasyim, A., Andri, K. B., … Hakim, A. L. (2019). Impact of starter solution technology on the use of fertilizers in production of chilli (Capsicum frutescens L.). IOP Conference Series: Earth and Environmental Science, 230(1), 12063. IOP Publishing.

Malusá, E., Sas-Paszt, L., & Ciesielska, J. (2012). Technologies for Beneficial Microorganisms Inocula Used as Biofertilizers. The Scientific World Journal, 2012, 1–12. DOI

Malusá, E., & Vassilev, N. (2014). A contribution to set a legal framework for biofertilisers. Applied Microbiology and Biotechnology, 98(15), 6599–6607. DOI

Panda, H. (2011). Manufacture of biofertilizer and organic farming. Asia Pacific Business Press Inc. Retrieved from website

Uthayasooriyan, M., Pathmanathan, S., Ravimannan, N., & Sathyaruban, S. (2016). Formulation of alternative culture media for bacterial and fungal growth. Der Pharmacia Lettre, 8(1), 431–436. Retrieved from website

Pop, O. L., Diaconeasa, Z., Thorsten, B., Ciuzan, O., Pamfil, D., Vodnar, D. C., & Socaciu, C. (2015). Effect of glycerol, as cryoprotectant in the encapsulation and freeze drying of microspheres containing probiotic cells. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology, 72(1), 27–32. DOI

Prasetiyono, J., Tasliah, Aswidinnoor, H., & Moeljopawiro, S. (2003). Identifikasi marka mikrosatelit yang terpaut dengan sifat toleransi terhadap keracunan alumunium pada padi persilangan DUPA x ITA131. Jurnal Bioteknologi Pertanian, 8(2), 35–45. Retrieved from PDF

Prayogo, C., & Ihsan, M. (2018). Utilization of LCC (Legume Cover Crop) and bokashi fertilizer for the efficiency of Fe and Mn uptake of former coal mine land. Journal of Degraded and Mining Lands Management, 6(1), 1527–1537. DOI

Qin, H. & Huang, R. (2018). Auxin Controlled by Ethylene Steers Root Development. International Journal Molecular Science. 2018, 19 (11), 3656. DOI

Ratzke, C., & Gore, J. (2018). Modifying and reacting to the environmental pH can drive bacterial interactions. PLoS Biology, 16(3), 1–20. DOI

Rizqiani, N. F., Ambarwati, E.,& Yuwono, W. (2007). Pengaruh dosis dan frekuensi pemberian pupuk organik cair terhadap pertumbuhan dan hasil buncis (Phaseolus vulgaris L.) dataran rendah. Jurnal Ilmu Tanah Dan Lingkungan, 7(1), 43–53. Retrieved from PDF

Rivaliati, S., Suntari, R., & Prayogo, C. (2017). Dinamika N mineral akibat aplikasi pupuk NPK kebomas berbasis amonium dan nitrat 25-7-7 pada tanaman buncis. Jurnal Tanah Dan Sumberdaya Lahan, 4(1), 493–502. Retrieved from website

Sankaralingam, S., Eswaran, S., Boomi, B., Sundaram, V. M., & Shankar, T. (2014). Screening and growth characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa. Advances in Environmental Biology, 8(13), 673–680. Retrieved from website

Santosa, M., Maghfoer, M. D., & Tarno, H. (2017). The Influence of Organic and Inorganic Fertilizers on the Growth and Yield of Green Bean, Phaseolus vulgaris L. Grown in Dry and Rainy Season. AGRIVITA, Journal of Agricultural Science, 39(3), 296–302. DOI

Savci, S. (2012). An agricultural pollutant: chemical fertilizer. International Journal of Environmental Science and Development, 3(1), 73–80. DOI

Sinha, R. K., Herat, S., Chauhan, K., & Valani, D. (2009). Earthworms vermicompost: a powerful crop nutrient over the conventional compost & protective soil conditioner against the destructive chemical fertilizers for food safety and security. American-Eurasian Journal of Agricultural and Environmental Science, 5(5), 14–22. Retrieved from website

Sudjana, H. B. (2011). Pemanfaatan Crotalaria retusa (L.) dan “Kascing” sebagai pupuk organik untuk sayuran selada (Lactuca sativa). Majalah Ilmiah Solusi Unsika, 10(20), 1–6. Retrieved from DOI

Wang, L., Yang, F., Yaoyao E, Yuan, J., Raza W, Huang Q & Shen, Q. (2016). Long term application of bioorganic fertilizer improved soil biochemical properties and microbial communities of an apple orchard soil. Frontier in Microbiology 7. 1893. DOI

Zhao, G., Zeng, Q., Zhang, S., Zhong, Y., Wang, C., Chen, Y., … Liao, Z. (2019). Effect of carrier lipophilicity and preparation method on the properties of andrographolide–solid dispersion. Pharmaceutics, 11(2), 74. DOI

DOI: http://doi.org/10.17503/agrivita.v42i1.2263


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