Efficacy of Selected Plant Extracts to Control Leaf Miner (Lyriomyza spp.) in Chrysanthemum

Indijarto Budi Rahardjo, Budi Marwoto, Kurniawan Budiarto


The use of eco-friendly methods in controlling pest and diseases has become an increasing concern in production system in many economically important crops, including ornamentals. The application of plant extract that has an insecticidal effect is considered as one promising alternative in reducing the negative effects of synthetic pesticides. The research was conducted to assess selected plant extracts in several concentrations against leaf miner (Lyriomyza spp.) in chrysanthemum. The experiment was carried out from January to December 2017 under plastic house conditions at the Indonesian Ornamental Crops Research Institute (IOCRI). The extracted organ of insecticidal plants regularly sprayed into chrysanthemum plants and compared with water as the controls. The results showed that the application of insecticidal plants extracts reduced attack intensity and incidence from water treatment (control). Certain treatment combinations, Chinese mahogany leaf extract at the concentration of 0.25%, C. pyrethrum petal 0.15 and 0.30%, and chinaberry leaf at 0.3, 0.35% even had 0.9-3.13% lesser attack incidences than commercial botanical insecticide Neem Plus. The respected treatments also supressed more than 62% leaf miner attacks and induced the increase of marketable flowers.


Biological control; Chrysanthemum; Efficacy; Lyriomyza spp; Plant extracts

Full Text:



Ahmadi, M., Amiri-Besheli, B., & Hosieni, S. (2012). Evaluating the effect of some botanical insecticides on the citrus mealybug Planococcus citri (Risso) (Hemiptera: Pseudococcidae). African Journal of Biotechnology, 11(53), 11620–11624. https://doi.org/10.5897/AJB11.4226

Akashe, V. B., Gud, M. A., Shinde, S. K., & Deshpande, A. N. (2009). Bio-efficacy of botanicals and chemical insecticides for the control of castor leaf miner (Liriomyza trifolii Burgess) under dry land condition. International Journal of Plant Protection, 2(2), 248–250. Retrieved from http://www.researchjournal.co.in/upload/assignments/2_248-250.pdf

Alves, D. S., Oliveira, D. F., Carvalho, G. A., Carvalho, D. ., Souza, L. P., & Lasmar, O. (2013). Selection of active plant extracts against the coffee leaf miner Leucoptera coffeella (Lepidoptera: Lyonetiidae). Revista Brasileira de Plantas Medicinais, 15(3), 352–362. https://doi.org/10.1590/s1516-05722013000300007

Andersen, A., An, T. T. T., & Nordhus, E. (2008). Distribution and importance of polyphagous Liriomyza species (Diptera, Agromyzidae) in vegetables in Vietnam. Norwegian Journal of Entomology, 55, 149–164. Retrieved from http://www.entomologi.no/journals/nje/2008-2/pdf/NJE-vol55-nr2-Andersen.pdf

Ateyyat, M. A., & Abu-Darwish, M. S. (2009). Short communication. Insecticidal activity of different extracts of Rhamnus dispermus (Rhamnaceae) against peach trunk aphid, Pterochloroides persicae (Homoptera: Lachnidae). Spanish Journal of Agricultural Research, 7(1), 160–164. https://doi.org/10.5424/sjar/2009071-415

Barati, R., Golmohammadi, G., Ghajarie, H., Zarabi, M., & Mansouri, R. (2013). The effects of some botanical insecticides and pymetrozine on life table parameters of silver leaf whitefly Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae). Pesticidi i Fitomedicina, 28(1), 47–55. https://doi.org/10.2298/pif1301047b

Chow, A., & Heinz, K. M. (2006). Control of Liriomyza langei on chrysanthemum by Diglyphus isaea produced with a standard or modified parasitoid rearing technique. Journal of Applied Entomology, 130(2), 113–121. https://doi.org/10.1111/j.1439-0418.2005.01028.x

da Costa-Lima, T. C., das Chagas, M. C. M., & Parra, J. R. P. (2014). Temperature-dependent development of two neotropical parasitoids of Liriomyza sativae (Diptera: Agromyzidae). Journal of Insect Science, 14(1), 245. https://doi.org/10.1093/jisesa/ieu107

de Evert, M. B. T., López, V. A. G., & de López, M. B. R. (2015). Evaluation of plant extracts for the control of tomato leaf miner Tuta absoluta (Meyrick) growing undergreen house. Investigación Agraria, 17(2), 138–142. https://doi.org/10.18004/investig.agrar.2015.diciembre.138-142

El-Wakeil, N. E. (2013). Botanical pesticides and their mode of action. Gesunde Pflanzen, 65, 125–149. https://doi.org/10.1007/s10343-013-0308-3

Foba, C. N., Salifu, D., Lagat, Z. O., Gitonga, L. M., Akutse, K. S., & Fiaboe, K. K. M. (2015). Species composition, distribution, and seasonal abundance of Liriomyza leafminers (Diptera: Agromyzidae) under different vegetable production systems and agroecological zones in Kenya. Environmental Entomology, 44(2), 223–232. https://doi.org/10.1093/ee/nvu065

Ismaeel, M., Hameed, M. S., Ahmed, N., Saif-Ur-Rehman, & Ahmed, S. (2016). Survey of the distribution of leaf miners on vegetables and other alternative host plants. Journal of Entomology and Zoology Studies, 4(4), 275–284. Retrieved from http://www.entomoljournal.com/archives/?-year=2016&vol=4&issue=4∂=D&ArticleId=1051

Jayanthi, P. D. K., & Verghese, A. (2007). Synergistic effect of insecticide-botanical mixtures on citrus leaf miner, Phyllocnistis citrella stainton. Pest Management in Horticultural Ecosystems, 13(2), 128–133. Retrieved from https://www.indianjournals.com/ijor.aspx?target=ijor:pmhe&volume=13&issue=2&article=007

Jones, V. P., Parrella, M. P., & Hodel, D. R. (1986). Biological control of leafminers in greenhouse chrysanthemums. California Agriculture, pp. 10–12. Retrieved from https://www.google.co.id/url?sa=t&rct=j&q=&esrc=s&source=web&cd=1&cad=rja&uact=8&ved=2ahUKEwjxhYKC9tzmAhXLfX0KHYfkAB8QFjAAegQIARAC&url=https%3A%2F%2Fucanr.edu%2Frepository%2FfileAccessPublic.cfm%3Ffn%3Dca4001p10-171758.pdf&usg=AOvVaw3DwoH8f7TTJhnza64eVRgW

Karunaratne, U. K. P. R., & Karunaratne, M. M. S. C. (2012). Evaluation of methanol, ethanol and acetone extracts of four plant species as repellents against Callosobruchus maculatus (Fab.). Vidyodaya Journal of Science, 17(1), 1–8. Retrieved from http://journals.sjp.ac.lk/index.php/vjs/article/view/2462

Li, G. (2012). Effect of botanical insecticide of Macleya cordata on physiology and biochemistry of cabbage (Brassica oleracea L.). IERI Procedia, 3, 156–161. https://doi.org/10.1016/j.ieri.2012.09.026

Marsh, T. L., & Gallardo, K. (2009). Adopting biological control for ornamental crops in greenhouses. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources, 4(022), 1–9. https://doi.org/10.1079/PAVSNNR20094022

Mujica, N., & Kroschel, J. (2011). Leafminer fly (Diptera: Agromyzidae) occurrence, distribution, and parasitoid associations in field and vegetable crops along the Peruvian coast. Environmental Entomology, 40(2), 217–230. https://doi.org/10.1603/en10170

Mujica, N., Valencia, C., Ramírez, L., Prudencio, C., & Kroschel, J. (2009). Temperature-dependent development of three parasitoids of the leafminer fly Liriomyza huidobrensis. In 15th International Symposium of the International Society for Tropical Root Crops (pp. 171–177). Retrieved from http://www.istrc.org/images/Documents/Symposiums/Fifthteenth/s7_mujica.pdf

Nonci, N., & Muis, A. (2011). Bioekologi dan pengendalian pengorok daun Liriomyza chinensis Kato (Diptera: Agromyzidae) pada bawang merah. Jurnal Penelitian Dan Pengembangan Pertanian, 30(4), 148–155. https://doi.org/10.21082/jp3.v30n4.2011.p148-155

Pavela, R. (2009). Effectiveness of some botanical insecticides against Spodoptera littoralis Boisduvala (Lepidoptera: Noctudiae), Myzus persicae Sulzer (Hemiptera: Aphididae) and Tetranychus urticae Koch (Acari: Tetranychidae). Plant Protection Science, 45(4), 161–167. https://doi.org/10.17221/16/2009-PPS

Puripattanavong, J., Songkram, C., Lomlim, L., & Amnuaikit, T. (2013). Development of concentrated emulsion containing Nicotiana tabacum extract for use as pesticide. Journal of Applied Pharmaceutical Science, 3(11), 016–021. https://doi.org/10.7324/JAPS.2013.31104

Rahardjo, I. B., Budiarto, K., & Marwoto, B. (2019). Evaluation of biological and chemical fungicides to control white rust in chrysanthemum grown under open condition. AGRIVITA Journal of Agricultural Science, 41(1), 55–63. https://doi.org/10.17503/agrivita.v41i1.1787

Ribeiro, A. V., Luz, C. E. A., Bastos, C. S., Krieger, Y. S. T., da Silva, N. H., & da Silva, W. B. (2017). Toxicity of botanical and synthetic formulations to the maize weevil, Sitophilus zeamais (Coleoptera: Curculionidae). Revista Colombiana de Entomologia, 43(2), 167–172. https://doi.org/10.25100/socolen.v43i2.5938

Sanchez, C., Gallego, J. R., Gamez, M., & Cabello, T. (2014). Intensive biological control in Spanish greenhouses: problems of the success. International Journal of Agricultural and Biosystems Engineering, 8(10), 1015–1019. https://doi.org/10.5281/zenodo.1096489

Shareef, M. F., Raza, A. B. M., Majeed, M. Z., Ahmed, K. S., Raza, W., & Hussain, H. F. (2016). Effect of botanicals on the infestation of citrus leaf miner, Phyllocnistis citrella stainton. Journal of Entomology and Zoology Studies, 4(4), 1335–1340. Retrieved from http://www.entomoljournal.com/archives/?year=2016&vol=4&issue=4&ArticleId=1153

Sharma, A., Diwevidi, V. D., Singh, S., Pawar, K. K., Jerman, M., Singh, L. B., … Srivastawa, D. (2013). Biological control and its important in agriculture. International Journal of Biotechnology and Bioengineering Research, 4(3), 175–180. Retrieved from https://www.ripublication.com/ijbbr_spl/ijbbrv4n3spl_03.pdf

Shawan, S. I., Rashed, R. U., Mitu, A. S., & Jahan, M. (2018). Efficacy of different chemical and botanical insecticides in controlling mango hopper (Amritodusatkinsoni L.). Advances in Plants & Agriculture Research, 8(2), 127–131. https://doi.org/10.15406/apar.2018.08.00301

Shinde, S. S., Neharkar, P. S., Dhurve, N. G., Sawai, H. R., Lavhe, N. V, & Masolkar, D. S. (2017). Evaluation of different insecticides against citrus leaf miner on Nagpur mandarin. Journal of Entomology and Zoology Studies, 5(6), 1889–1892. Retrieved from http://www.entomoljournal.com/archives/2017/vol5issue6/PartZ/5-6-26-898.pdf

Tomczyk, A., Kropczyńska, D., Ptak, A., Titova, M., Shumiło, N., Oreshnikov, A., … Furmanowa, M. (2007). Effect of Polyscias filicifolia bailey extracts on the behavior of the leaf miner Cameraria ohridella deschka and dimic on horse chestnut trees. Acta Biologica Cracoviensia Series Botanica, 49(1), 55–60. Retrieved from http://citeseerx.ist.psu.edu/viewdoc/download?-doi=

Xavier, V. M., Message, D., Picanço, M. C., Chediak, M., Santana Júnior, P. A., Ramos, R. S., & Martins, J. C. (2015). Acute toxicity and sublethal effects of botanical insecticides to honey bees. Journal of Insect Science, 15(1), 137. https://doi.org/10.1093/jisesa/iev110

Zhong, B., Lv, C., & Qin, W. (2017). Effectiveness of the botanical insecticide azadirachtin against Tirathaba rufivena (Lepidoptera: Pyralidae). Florida Entomologist, 100(2), 215–218. https://doi.org/10.1653/024.100.0215

DOI: http://doi.org/10.17503/agrivita.v42i1.2219

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.