Conidial Density and Viability of Beauveria bassiana Isolates from Java and Sumatra and Their Virulence Against Nilaparvata lugens at Different Temperatures

Eka Sumikarsih, Siti Herlinda, Yulia Pujiastuti

Abstract


The brown planthopper (BPH), Nilaparvata lugens can cause direct damage and transmit rice diseases.  Beauveria bassiana is used to control BPH, however the success of the fungal efficacy on rice fields is affected by external factors, such as temperature. This research aimed to evaluate the conidial viability and density of B. bassiana isolates from Java and Sumatra, exposed to 25 and 34°C and their virulence against BPH nymphs. Twenty six isolates of B. bassiana cultures incubated at 25 and 34°C for 7 days were observed on their conidial density, viability, and  virulence against BPH nymphs. The incubation temperature of 34°C was able to decrease conidial density and viability, and virulence of the isolates.  However, some isolates of B. bassiana originated from soils or insects in Sumatra, especially from South Sumatra still produced high conidial density and viability as well as high virulent against BPH nymphs, such as TS1D3A, TSID3B, TS1D2A and TS1D2B isolates. The TS1D2B isolate incubated at 34°C still caused the highest percentage of BPH mortality (43.33%)  among other isolates. Therefore, the isolates can be used as promosing candidate for  biocontrol for N. lugens on rice planted in tropical ecosystem, such as wetland or lowland rice ecosystems in Indonesia.

Keywords


Biocontrol; Brown planthopper; Entomopathogenic fungus; Mortality

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References


Baehaki, S. E., & Suparno, H. (2018). Efficacy and induce resurgence of insecticides mixture of buprofezin 100 g/l + pymetrozine 50% to brown planthopper, Nilaparvata lugens (Stål) on higher initial population in the ricefield. International Journal of Entomology Research, 3(1), 35–42. Retrieved from pdf

Bugeme, D. M., Maniania, N. K., Knapp, M., & Boga, H. I. (2008). Effect of temperature on virulence of Beauveria bassiana and Metarhizium anisopliae isolates to Tetranychus evansi. In J. Bruin & L. P. S. van der Geest (Eds.), Diseases of Mites and Ticks (pp. 275–285). Dordrecht: Springer. crossref

Butt, T. M., Ibrahim, L., Ball, B. V., & Clark, S. J. (1994). Pathogenicity of the entomogenous fungi Metarhizium anisopliae and Beauveria bassiana against crucifer pests and the honey bee. Biocontrol Science and Technology, 4(2), 207–214. crossref

Catiding, J. L. A., Arida, G. S., Baehaki, S. E., Bentur, J. S., Cuong, L. Q., Norowi, M., … Lu, Z. (2009). Situation of planthopper in Asia. In K. L. Heong & B. Hardy (Eds.), Planthoppers: New Threats to the Sustainability of Intensive Rice Production System in Asia (pp. 191–220). Los Baños: International Rice Research Institute. Retrieved from pdf

Chinniah, C., Ravikumar, A., Kalyanasundaram, M., & Parthiban, P. (2016). Field evaluation of Metarhizium anisopliae liquid formulation (Bio-Magic®) against brown plant hopper, Nilaparvata lugens Stal on rice. Journal of Biopesticides, 9(2), 211–219. Retrieved from pdf

Constanski, K. C., Neves, P. M. O. J., Nogueira, L. M., Santoro, P. H., Amaro, J. T., & Zorzetti, J. (2011). Selection and evaluation of virulence of Beauveria bassiana (Bals.) Vuill. submitted to different temperature. Semina: Ciências Agrárias, 32(3), 875–882. crossref

Daravath, V., & Chander, S. (2017). Niche regulation between brown planthopper (BPH) and white backed planthopper (WBPH) in association with their natural enemy population in the rice ecosystem. Journal of Entomology and Zoology Studies, 5(5), 513–517. Retrieved from pdf

Dharshini, G. M., & Siddegowda, D. K. (2015). Reaction of rice landraces against brown planthopper Nilaparvata lugens Stal. The Ecoscan, 9(1&2), 605–609. Retrieved from pdf

Dietzgen, R. G., Mann, K. S., & Johnson, K. N. (2016). Plant virus-insect vector interactions: Current and potential future research directions. Viruses, 8(11), 303. crossref

Fernandes, É. K. K., Rangel, D. E. N., Moraes, Á. M. L., Bittencourt, V. R. E. P., & Roberts, D. W. (2007). Variability in tolerance to UV-B radiation among Beauveria spp. isolates. Journal of Invertebrate Pathology, 96(3), 237–243. crossref

Firouzbakht, H., Zibaee, A., Hoda, H., & Sohani, M. M. (2015). Virulence determination of Beauveria bassiana isolates on a predatory hemipteran, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae). Acta Phytopathologica et Entomologica Hungarica, 50(1), 115–125. crossref

Ghany, T. M. A. (2015). Entomopathogenic fungi and their role in biological control. OMICS International. crossref

Gholamzadeh-Chitgar, M., Hajizadeh, J., Ghadamyari, M., Karimi-Malati, A., & Hoda, H. (2017). Effect of sublethal concentration of Beauveria bassiana fungus on demographic and some biochemical parameters of predatory bug, Andrallus spinidens Fabricius (Hemiptera: Pentatomidae) in laboratory conditions. Trakia Journal of Science, 15(2), 160–167. crossref

Herlinda, S., Yudha, S., Thalib, R., Khodijah, Suwandi, Lakitan, B., & Verawaty, M. (2018). Species richness and abundance of spiders inhabiting rice in fresh swamps and tidal lowlands in South Sumatra, Indonesia. Journal of the International Society for Southeast Asian Agricultural Sciences, 24(1), 82–93. Retrieved from website

Hu, G., Lu, F., Zhai, B. P., Lu, M. H., Liu, W. C., Zhu, F., … Zhang, X. X. (2014). Outbreaks of the brown planthopper Nilaparvata lugens (Stål) in the yangtze river delta: Immigration or local reproduction? PLoS ONE, 9(2), e88973. crossref

Leavengood, J. M., Bartlett, C. R., & Vitanza-Hedman, S. (2017). First reports of six planthoppers (Hemiptera: Fulgoroidea: Tagosodes, Delphacodes, Pareuidella, Nilaparvata, Asarcopus, Bruchomorpha) in Texas. Entomological News, 127(3), 215–229. Retrieved from crossref

Lee, S. J., Yu, J. S., Nai, Y. S., Parker, B. L., Skinner, M., & Kim, J. S. (2015). Beauveria bassiana sensu lato granules for management of brown planthopper, Nilaparvata lugens in rice. BioControl, 60(2), 263–270. crossref

Li, M., Li, S., Xu, A., Lin, H., Chen, D., & Wang, H. (2014). Selection of Beauveria isolates pathogenic to adults of Nilaparvata lugens. Journal of Insect Science, 14(1), 32. crossref

Li, M.-Y., Lin, H.-F., Li, S.-G., Xu, A.-M., & Feng, M.-F. (2012). Efficiency of entomopathogenic fungi in the control of eggs of the brown planthopper Nilaparvata lugens Stål (Homopera: Delphacidae). African Journal of Microbiology Research, 6(44), 7162–7167. crossref

Liu, J., Zhang, J., Qin, X., Chen, Y., Yuan, F., & Zhang, R. (2013). Toxic effects of pymetrozine on the brown planthopper, Nilaparvata lugens (Stål) (Homoptera: Delphacidae). Journal of Entomological Science, 48(1), 17–22. crossref

Lohse, R., Jakobs-Schönwandt, D., & Patel, A. V. (2014). Screening of liquid media and fermentation of an endophytic Beauveria bassiana strain in a bioreactor. AMB Express, 4, 47. crossref

Nuraini, F. R., Setyaningsih, R., & Susilowati, A. (2017). Screening and characterization of endophytic fungi as antagonistic agents toward Fusarium oxysporum on eggplant (Solanum melongena). Biodiversitas, 18(4), 1377–1384. crossref

Oliveira, D. G. P., Pauli, G., Mascarin, G. M., & Delalibera, I. (2015). A protocol for determination of conidial viability of the fungal entomopathogens Beauveria bassiana and Metarhizium anisopliae from commercial products. Journal of Microbiological Methods, 119, 44–52. crossref

Ottati-de-Lima, E. L., Batista Filho, A., de Almeida, J. E. M., Gassen, M. H., Wenzel, I. M., de Almeida, A. M. B., & Zapellini, L. O. (2014). Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules. Arquivos Do Instituto Biológico, 81(4), 342–350. crossref

Pham, T. A., Kim, J. J., Kim, S. G., & Kim, K. (2009). Production of blastospore of entomopathogenic Beauveria bassiana in a submerged batch culture. Mycobiology, 37(3), 218–224. crossref

Safitri, A., Herlinda, S., & Setiawan, A. (2018). Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas, 19(6), 2365–2373. crossref

Salim, H., Md. Rawi, C. S., Ahmad, A. H., & Al-Shami, S. A. (2015). Efficacy of insecticide and bioinsecticide ground sprays to control Metisa plana walker (Lepidoptera: Psychidae) in oil palm plantations, Malaysia. Tropical Life Sciences Research, 26(2), 73–83. Retrieved from crossref

Satpathi, C. R., Acharjee, P., & Saha, J. (2016). Natural mycosis of rice brown plant hopper (Nilaparvata lugens Stål) in Eastern India. American Scientific Research Journal for Engineering, Technology, and Sciences, 26(4), 195–204. Retrieved from crossref

Siaga, E., Lakitan, B., Hasbi, H., Bernas, S. M., Widuri, L. I., & Kartika, K. (2019). Floating seedbed for preparing rice seedlings under unpredictable flooding occurrence at tropical riparian wetland. Bulgarian Journal of Agricultural Science, 25(2), 326–336. pdf

Tefera, T., & Pringle, K. (2003). Germination, radial growth, and sporulation of Beauveria bassiana and Metarhizium anisopliae isolates and their virulence to Chilo partellus (Lepidoptera: Pyralidae) at different temperatures. Biocontrol Science and Technology, 13(7), 699–704. crossref

Trizelia, & Nurdin, F. (2010). Virulence of entomopathogenic fungus Beauveria bassiana isolates to Crocidolomia pavonana F (Lepidoptera: Crambidae). AGRIVITA Journal of Agricultural Science, 32(3), 254–260. Retrieved from website

Zhang, X., Liu, X., Zhu, F., Li, J., You, H., & Lu, P. (2014). Field evolution of insecticide resistance in the brown planthopper (Nilaparvata lugens Stål) in China. Crop Protection, 58, 61–66. crossref

Zheng, L., Mao, Q., Xie, L., & Wei, T. (2014). Infection route of rice grassy stunt virus, a tenuivirus, in the body of its brown planthopper vector, Nilaparvata lugens (Hemiptera: Delphacidae) after ingestion of virus. Virus Research, 188, 170–173. crossref




DOI: http://doi.org/10.17503/agrivita.v41i2.2105

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