QTLs CONTROLLING SEED WEIGHT AND DAYS TO FLOWERING IN MUNGBEAN [Vigna radiata (L.) Wilczek], THEIR CONSERVATION IN AZUKI BEAN [V. angularis (Ohwi) Ohwi & Ohashi] AND RICE BEAN [V. umbellata (Thunb.) Ohwi & Ohashi]

Prakit Somta, Sompong Chankaew, Alisa Kongjaimun, Peerasak Srinives

Abstract


Mungbean (Vigna radiata (L.) Wilczek) is a socio-economically important legume crop of Asia. Varieties with large seed size and early maturity are preferred in commercial production. In this study, we identified quantitative trait loci (QTL) controlling seed weight and days to flowering in mungbean. The mapping population comprises 155 F2-derived lines from a cross between Kamphaeng Saen 1 (large-seeded and early flowering) and V4718 (small-seeded and late flowering). The F2 population was analyzed with 67 simple sequence repeat markers. The F2:3 families were evaluated for 100-seed weigh and days to flowering in two years, 2008 (one season) and 2011 (two seasons). Composite interval mapping identified six QTLs for 100-seed weight and 5 QTLs for days to flowering. Three genomic regions harbored QTLs for both seed weight and days to flowering, revealing association between the two traits. Comparison of QTLs for both traits found in this study with those reported in azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi) and rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) revealed that several QTLs are conserved among the three Vigna species. 


Keywords


comparative mapping; flowering; mungbean; quantitative trait loci; seed size

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References


Blair, M.W., F. Pedraza, H.F. Buendia, E.Gaitán-Solís, S.E. Beebe, P. Gepts and J. Tohme. 2003. Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 107: 1362–1374.

Buso, G.S.C., Z.P.S. Amaral, R.P.V. Brondani and M.E. Ferreira. 2006. Microsatellite markers for the common bean Phaseolus vulgaris. Mol. Ecol. Notes. 6: 252–254.

Fatokun, C.A., D.I. Menancio-Hautea, D. Danesh and N.D. Young. 1992. Evidence for orthologous seed weight genes in cowpea and mung bean based on RFLP mapping. Genetics. 132 (3): 841–846.

Fernandez, G.C.J. and S. Shanmugasundaram. 1988. The AVRDC mungbean improvement program: The past, present and future. In: Shanmugasundaram, S. and B.T. McLean. (Eds.) Mungbean. Proceeding of the Second International Symposium. Asian Vegetable Research and Development Center. Taiwan. p. 55-70.

Gaitán-Solís, E., M.C. Duque, K.J. Edwards and J. Tohme. 2002. Microsatellite repeats in common bean (Phaseolus vulgaris): Isolation, characterization, and cross-species amplification in Phaseolus ssp. Crop Sci. 42 (6): 2128–2136.

Guerra-Sanz, J.M. 2004. New SSR markers of Phaseolus vulgaris from sequence databases. Plant Breeding. 123 (1): 87–89.

Gwag, J.G., J.W. Chung, H.K. Chung, J.H. Lee, K.H. Ma, A. Dixit, Y.J. Park, E.G. Cho, T.S. Kim and S.H. Lee. 2006. Characterization of new microsatellite markers in mung bean, Vigna radiata (L.). Mol. Ecol. Notes. 6 (4): 1132–1134.

Han, O.K., A. Kaga, T. Isemura, X.W. Wang, N. Tomooka and D.A. Vaughan. 2005. A genetic linkage map for azuki bean [Vigna angularis (Willd.) Ohwi & Ohashi]. Theor. Appl. Genet. 111: 1278–1287.

Humphry, M.E., C.J. Lambrides, S.C. Chapman, E.A.B. Aitken, B.C. Imrie, R.J. Lawn, C.L. MacIntyre and C.J. Liu. 2005. Relationships between hard-seededness and seed weight in mungbean (Vigna radiata) assessed by QTL analysis. Plant Breeding. 124: 292–298.

Imrie, B.C., Z.U. Ahmed and J.P.J. Eerens. 1985. Heritability of seed weight in mungbean. SABRAO J. Breed. Genet. 17: 173–175.

Isemura, T., A. Kaga, S. Konishi, T. Ando, N. Tomooka, O.K. Han and D.A. Vaughan. 2007. Genome dissection of traits related to domestication in azuki bean (Vigna angularis) and comparison with other warm-season legumes. Ann. Bot. 100 (5): 1053–1071.

Isemura, T., A. Kaga, N. Tomooka, T. Shimizu and D.A. Vaughan. 2010. The genetics of domestication of rice bean, Vigna umbellata. Ann. Bot. 106 (6): 927–944.

Kaga, A., T. Isemura, N. Tomooka and D.A. Vaughan. 2008. The genetics of domestication of the azuki bean (Vigna angularis). Genetics. 178 (2): 1013–1036.

Kongjaimun, A., A. Kaga, N. Tomooka, P. Somta, D.A. Vaughan and P. Srinives. 2012. The genetics of domestication of yardlong bean, Vigna unguiculata (L.) Walp. ssp. unguiculata cv.-gr. sesquipedalis. Ann. Bot. 109 (6): 1185-1200.

Kosambi, D.D. 1943. The estimation of map distances from recombination values. Ann. Eugen. 12 (1): 172–175.

Li, C.D., C.A. Fatokun, B. Ubi, B.B. Singh and G.J. Scoles. 2001. Determining genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers. Crop Sci. 41 (1): 189–197.

Lodhi, M.A., G.N. Ye, N.F. Weeden and B.I. Reisch. 1994. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol. Biol. Rep. 12 (1): 6–13.

Poehlman, J.M. 1990. The mungbean. Oxford and IBH Publishing Co. Pvt. Ltd. New Delhi.

Seehalak, W., P. Somta, W. Sommanas and P. Srinives. 2009. Microsatellite markers for mungbean developed from sequence database. Mol. Ecol. Resour. 9 (3): 862–864.

Siddique, M., M.F.A. Malik and S.I. Awan. 2006. Genetic divergence, association and performance evaluation of different genotypes of mungbean (Vigna radiata). Int. J. Agric. Biol. 8 (6): 793–795.

Sompong, U., P. Somta, V. Raboy and P. Srinives. 2012. Mapping of quantitative trait loci for phytic acid and phosphorus contents in seed and seedling of mungbean (Vigna radiata (L.) Wilczek). Breed. Sci. 62 (1): 87–92.

Somta, P., A. Kaga, N. Tomooka, K. Kashiwaba, T. Isemura, B. Chaitieng, P. Srinives and D.A. Vaughan. 2006. Development of an interspecific Vigna linkage map between Vigna umbellata (Thunb.) Ohwi & Ohashi and V. nakashimae (Ohwi) Ohwi & Ohashi and its use in analysis of bruchid resistance and comparative genomics. Plant Breeding. 125 (1): 77–84.

Somta, P. and P. Srinives. 2007. Genome research in mungbean (Vigna radiata (L.) Wilczek) and blackgram (V. mungo (L.) Hepper). Sci. Asia. 33 (Supplement 1): 69–74.

Somta, P., W. Musch, B. Kongsamai, S. Chanprame, S. Nakasathien, T. Toojinda, W. Sorajjapinun, W. Seehalak, S. Tragoonrung and P. Srinives. 2008. New microsatellite markers isolated from mungbean (Vigna radiata (L.) Wilczek). Mol. Ecol. Resour. 8 (5): 1155–1157.

Somta, P., W. Seehalak and P. Srinives. 2009. Development, characterization and cross-species amplification of mungbean (Vigna radiata) genic microsatellite markers. Conserv. Genet. 10: 1939–1943.

Tangphatsornruang, S., P. Somta, P. Uthaipaisanwong, J. Chanprasert, D. Sangsrakru, W. Seehalak, W. Sommanas, S. Tragoonrung and P. Srinives. 2009. Characterization of microsatellites and gene contents from genome shotgun sequences of mungbean (Vigna radiata (L.) Wilczek). BMC Plant Biol. 9: 137.

Wang, X.W., A. Kaga, N. Tomooka and D.A. Vaughan. 2004. The development of SSR markers by a new method in plants and their application to gene flow studies in azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi). Theor. Appl. Genet. 109: 352–360.

Wang, S., C.J. Basten, and Z.B. Zeng. 2011. Windows QTL cartographer 2.5. Department of Statistics. North Carolina State University. Raleigh. North Caroline.

Yimram, T., P. Somta and P. Srinives. 2009. Genetic variation in cultivated mungbean germplasm and its implication in breeding for high yield. Field Crops Res. 112 (2-3): 260–266.




DOI: http://doi.org/10.17503/agrivita.v37i2.578

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