Dwi Hapsoro, Hayane Adeline Warganegara, Setyo Dwi Utomo, Nanik Sriyani, Yusnita Yusnita


This experiment was conducted to reveal genetic diversity among 38 genotypes of sugarcane (Saccharum officinarum L.) using RAPD markers. The population consisted of 8 genotypes from Australia, 7 from Africa, 10 from America, and 13 from Asia. Genetic similarity was ranging from 17% to 97% , with the average of 57%.  UPGMA dendrograms divided the population into three major groups i.e. group 1, 2, and 3 which consist-ed of 23, 10, and 5 genotypes, respectively. Each major group comprised genotypes of different geographical origins. The dendrogram divided each group into some subgroups. There were 8 subgroups i.e. 4 subgroups in group 1, 2 sub-groups in group 2, and 2 subgroups in group 3.  Some genotypes of same geographical origin were clustered into in at least 3 different sub-groups, meaning that they were genetically dis-similar. On the other hand, some other geno-types of different geographical origin were clustered into the same subgroup, meaning that they were genetically similar. This data would help sugarcane breeders to select parents for hybridization in order to maximize heterosis. This could be conducted by selecting parents of dis-similar genotypes.



diversity; RAPD; sugarcane

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Al-Saghir, M.G. and A.G.A. Salam. 2011. Genetic diversity of North American soybean (Glycine max L.) cultivars as revealed by RAPD markers. J. Plant Sci. 6 (1): 36-42. crossref

Amini, F., G. Saeidi and A. Arzani. 2008. Study of genetic diversity in safflower geno-types using agro-morphological traits and RAPD markers. Euphytica 163: 21-30. crossref

Anatala, T.J., H.P. Gajera, D.D. Savaliya, R.K. Domadiya, S.V. Patel and B.A. Golakiya. 2014. Molecular diversity analysis of cowpea (Vigna unguiculata L.) genotypes determined by ISSR and RAPD markers. Int. J. Agric. Environ. Biotechnol. 7 (2): 269-276. crossref

Arshad, S., A. Iqbal, S. Nawaz, and N. Ahmed. 2011. Genetic diversity studies of coarse and fine rice using RAPD markers. Front. Agric. China 5 (2): 129-134. crossref

Bhau, B.S., K. Medhi, A.P. Das, S.P. Saikia, K. Neog and S.N. Choudhury. 2009. Analy-sis of genetic diversity of Persea bomby-cina "Som" using RAPD-based molecu-lar markers. Biochem. Genet. 47: 486-497. crossref

Chen, S.Y., T.X. Dai, Y.T. Chang, S.S. Wang, S.L. Ou, W.L. Chuang, C.Y. Chuang, Y.H. Lin and H.M. Ku. 2013. Genetic diversity among “Ocimum” species based on ISSR, RAPD and SRAP markers. Aust. J. Crop Sci. 7 (10): 1463-1471.

Devarumath, R.M., S.B. Kalwade, P.G. Kawar and K.V. Sushir. 2012. Assessment of genetic diversity in sugarcane germ-plasm using ISSR and SSR markers. Sugar Tech. 14 (4): 334-344. crossref

Govindaraj, P., R. Sindhu, A. Balamurugan and C. Appunu. 2011. Molecular diversity in sugarcane hybrids (Saccharum spp. complex) grown in peninsular and east coast zones of tropical India. Sugar Tech. 13 (3): 206-213. crossref

Kawar, P.G., R.M. Devarumath and Y. Nerkar. 2009. Use of RAPD markers for assess-ment of genetic diversity in sugarcane cultivars. Indian J. Biotechnol. 8: 67-71.

Khan, F.A., A. Khan, F.M. Azhar and S. Rauf. 2009. Genetic diversity of Saccharum officinarum accessions in Pakistan as revealed by random amplified poly-morphic DNA. Genet. Mol. Res. 8 (4): 1376-1382. crossref

Lam, L.V., T. Thanh, V.T.Q. Chi and L.M. Tuy. 2009. Genetic diversity of Hevea IRRDB'81 collection assessed by RAPD markers. Mol. Biotechnol. 42 (3): 292-298. crossref

Nair, N.V. 2011. Sugarcane varietal develop-ment programmes in India: An overview. Sugar Tech. 13 (4): 275-280. crossref

Nair, N.V., A. Selvi, T.V. Sreenivasan and K.N. Pushpalatha. 2002. Molecular diversity in Indian sugarcane cultivars as revealed by randomly amplified DNA polymor-phisms. Euphytica 127: 219-225. crossref

Nei, M. 1978. Estimation of average hetero-zygosity and genetic distance from a small number of individuals. Genetics 89 (3): 583-590.

Rafii, M.Y., M. Shabanimofrad, M.W.P. Edaroyati and M.A. Latif. 2012. Analysis of the genetic diversity of physic nut, Jatropha curcas L. accessions using RAPD markers. Mol. Biol. Rep. 39 (6): 6505-6511. crossref

Rodríguez, J., P. Rodríguez, M.E. González and P. Martínez-Gómez. 2010. Molecular characterization of Cuban endemism Carica cubensis Solms using random amplified polymorphic DNA (RAPD) markers. Agr. Sci. 1 (3): 95-101. crossref

Samal, K.C., R.C. Jena, S.S. Swain, B.K. Das and P.K. Chand. 2012. Evaluation of genetic diversity among commercial cultivars, hybrids and local mango (Mangifera indica L.) genotypes of India using cumulative RAPD and ISSR markers. Euphytica 185 (2): 195-213. crossref

Santos, J.R.P., M.A. Teixeria, J.E. Cares, F.G. Faleiro and D.C. Costa. 2010. Contras-tant banana accessions for resistance to the burrowing nematode, based on mol-ecular markers RAPD. Euphytica 172 (1): 13-20. crossref

Saravanakumar, K., P. Govindaraj, C. Appunu, S. Senthilkumar and R. Kumar. 2014. Analysis of genetic diversity in high biomass producing sugarcane hybrids (Saccharum spp. complex) using RAPD and STMS markers. Indian J. Biotechnol. 13: 214-220.

Sen, S., R. Skaria and P.M.A. Muneer. 2010. Genetic diversity analysis in Piper species (Piperaceae) using RAPD markers. Mol. Biotechnol. 46 (1): 72-79. crossref

Tabasum, S., F.A. Khan, S. Nawaz, M.Z. Iqbal and A. Saeed. 2010. DNA profiling of sugarcane genotypes using randomly amplified polymorphic DNA. Genet. Mol. Res. 9 (1): 471-483. crossref

Thul, S.T., M.P. Darokar, A.K. Shasany and S.P.S. Khanuja. 2012. Molecular profiling for genetic variability in Capsicum species based on ISSR and RAPD markers. Mol. Biotechnol. 51 (2): 137-147. crossref

Vaze, A., G. Nerkar, M. Pagariya, R.M. Devarumath and D.T. Prasad. 2010. Isolation and PCR amplification of genomic DNA from dry leaf samples of sugarcane. Int. J. Pharma Bio Sci. 1 (2): 1-6.

Wangsomnuk, P.P., S. Khampa, S. Jogloy, T. Srivong, A. Patanothai and Y.B. Fu. 2011. Assessing genetic structure and relatedness of jerusalem artichoke (Helianthus tuberosus L.) germplasm with RAPD, ISSR and SRAP markers. Am. J. Plant Sci. 2: 753-764. crossref


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