Evaluation of BiomeFert Biofertilizer for Growth and Yield Improvement in Glycine Max L. Varieties Dega-1

Fatimah Fatimah, Elsha Nadya Marchelia Putri, Syarifah Salsabila, Nazil Dwi Rahayuningtyas, Anastasya Nabila Putri Soelistyo, Wanda Dya Arneni, Princessa Nandita Febrionny, Agus Supriyanto, Nimatuzahroh Nimatuzahroh, Almando Geraldi

Abstract


Biofertilizer application can potentially reduce chemical residues in soil from synthetic fertilizers. This study investigates the effects of varying doses and application frequencies of biofertilizers on the growth, productivity, and Relative Agronomic Effectiveness (RAE) of Glycine max L. A Completely Randomized Design was employed, with 9 treatment combinations and 2 control groups (positive and negative), each replicated three times. The biofertilizer doses tested were 5, 10, and 15 ml per plant (B5, B10, B15), and application frequencies included 1 week after planting (WAP) (F1), at 1 and 4 WAP (F2), and at 1, 4, and 8 WAP (F3). The negative control received no biofertilizer, while the positive control received NPK (5 g/plant). Plant growth was assessed by measuring height and leaf number, and productivity was evaluated based on the number of pods, pod weight, dry seed weight, and weight per 100 seeds. Data were analyzed using one-way ANOVA. The results indicated that the treatment with 10 ml of biofertilizer applied twice (B10F2) significantly improved the growth and productivity of Glycine max L., achieving an RAE value of 169.70%.


Keywords


Biofertilizer Formula; Glycine max L.; Life on land; Relative Agronomic Effectiveness

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References


Adnan, Bakar, B. A., Azis, A., Rosa, E., Fitri, S., Savitri, S., & Fachruddin, F. (2021). The effects of phosphate and lime fertilizer on the growth and production of soybean plants in a former wetland. IOP Conference Series: Earth and Environmental Science, 667, 012045. DOI

Amutha, R., Karunakaran, S., Dhanasekaran, S., Hemalatha, K., Monika, R., Shanmugapriya, P., & Sornalatha, T. (2014). Isolation and mass production of biofertilizer (Azotobacter and Phosphobacter). International Journal of Latest Research in Science and Technology, 3(1), 79-81. PDF

Ariani, N. S., & Simarmata, T. (2023). A systematic review: Current technology of solid carrier formulation to improve viability and effectiveness of nitrogen-fixing Inoculant. Jurnal Agrikultura. 34(1), 48-57. DOI

Asyura AG, L., Hasanah, Y., & Irmansyah, T. (2018). Growth and production response of soybean (Glycine max (L.) Merrill) against drought stress treatment and provision of antioxidants salicylic acid and ascorbic acid. Jurnal Agroekoteknologi FP USU, 6(1), 174-179. website

BPS-Statistics Indonesia. (2024). Impor kedelai menurut negara asal utama, 2017-2023. Badan Pusat Statistik. website

Çakmakçı, R., Salık, M.A., & Çakmakçı, S. (2023). Assessment and principles of environmentally sustainable food and agriculture systems. Agriculture, 13, 1073. DOI

Caskiwan, Eris, F. R., Nurmayulis, & Denny, Y. R. (2019). Effect of organic matter of chicken manure with bioactivator application of microbial rhizosphere inoculant to growth and yield of soybean (Glycine max (L.) Merill). IOP Conf. Series: Earth and Environmental Science, 383, 012020. DOI

Fatimah, Annizah, I.N., Alawiyah, D.D., Susetyo, R.D., Surtiningsih, T., & Nurhariyati, T. (2021) Phosphate solubilizing bacteria isolated from Tuban mangrove soil Indonesia. IOP Conference Series: Earth and Environmental Science, 762(1), 012007. DOI

Fatimah, Fadilah, R. L. A., Millah, A. I., Nurhariyati, T., Irawan, B., Ni’matuzahroh, Affandi, M., Zuhri A. R. N. I., Widhiya, E. W., Salsabila, S., & Ramly, Z. A. (2022). Ability test of IAA (Indole-3-Acetic Acid) hormone-producing endophytic bacteria from Lamongan mangrove. Jurnal Riset Biologi dan Aplikasinya, 4(1), 42-50. DOI

Fatimah, Jannah, Z., Suroiyah, F., Azzah, Salamun, Nurhariyati, T., & Surtiningsih, T. (2020). Exploration of proteolytic bacteria from mangrove center Tuban. Annals of Biology, 36(2), 267-271.

Gasim, S. M., & Abdelmula, A. A. (2018). Impact of bee pollination on yield of faba bean (Vicia faba L.) grown under semi-arid conditions. Agricultural Sciences, 9, 729-740. DOI

Gonzalez, J.M., & Aranda, B. (2023). Microbial growth under limiting conditions-future perspectives. Microorganisms, 11(7), 1641. DOI

Harahap, R.T., Azizah, I.R., Setiawati, M.R., Herdiyantoro, D., & Simarmata, T. (2023). Enhancing upland rice growth and yield with indigenous plant growth-promoting rhizobacteria (PGPR) isolate at N-fertilizers dosage. Agriculture, 13, 1987. DOI

Hariyadi, B. W., Huda, N., & Nurlina. (2019). Analysis of growth and plantbaby kai-lan (Brassica alboglabra L.) the use of various doses of fertilizer urea. Journal of Agricultural Science and Agriculture Engineering, 3(1), 1-11. website

Hayati, N., Sudiarso, Prijono, S., & Aini, N. (2019). The effect of compost combined with phosphate solubilizing bacteria and nitrogen-fixing bacteria for increasing the growth and yield of chili plants. Russian Journal of Agricultural and Socio-Economic Sciences, 8(92), 287-292. DOI

Herawati, N., Aisah, A.R., & Hidayah, B.N. (2020). Growth and yield of four Indonesian improved soybean varieties based on bio-detas input package in rainfed lowland of Central Lombok. International Journal of Biosciences And Biotechnology, 7(2), 74-82. DOI

Jote, C.A. (2023). The impacts of using inorganic chemical fertilizers on the environment and human health. Organic and Medicinal Chemistry International Journal, 13(3), 555864. PDF

Kalay, A. M., Hindersah, R., Ngabalin, I. A., & Jamlean, M. (2020). Utilization of biofertilizers and organic materials on growth and yield of sweet corn (Zea mays-saccharata). Agriculture, 32(2), 129-138. DOI

Kanchana, P., Santha, M. L., & Raja, K. D. (2016). A review on Glycine max (L.) Merr. (soybean). World Journal of Pharmacy and Pharmaceutical Sciences, 5(1), 356-371. website

Karnwal, A., & Dohroo, A. (2018). Effect of maize root exudates on indole-3-acetic acid production by rice endophytic bacteria under influence of L-tryptophan. F1000Research, 7, 112. DOI

Khan, F., Siddique, A. B., Shabala, S., Zhou, M., & Zhao, C. (2023). Phosphorus plays key roles in regulating plants’ physiological responses to abiotic stresses. Plants, 12(15), 2861. DOI

Kugbe, J. X., Mawiya, W., Hafiz, A. M., & Maganoba, C. (2019). Increase in the use of organic fertilizers as complements to inorganic fertilizers in maintenance of soil fertility and environmental sustainability. World Journal of Agriculture and Soil Science, 4(1), 1-4. DOI

Li, W., Zhang, Y., Wang, C., Mao, W., Hang, T., Chen, M., & Zhang, B. (2013). How to evaluate the rice cultivation suitability?. Asian Agricultural Research, 5(12), 59-64. DOI

Minister of Agriculture. (2011). Pupuk organik, pupuk hayati dan pembenah tanah. Regulation of the Minister of Agriculture No.70/Permentan/Sr.140/ 10/2011. PDF

Mohite, B. (2013). Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and Its effect on plant growth. Journal of Soil Science and plant nutrition, 13(3), 638-649. DOI

Monzon, J. P., La Menza, N. C., Cerrudo, A., Canepa, M., Edreira, J. I. R., Specht, J., Andrade, F. H., & Grassini, P. (2021). Critical period for seed number determination in soybean as determined by crop growth rate, duration, and dry matter accumulation. Field Crops Research, 261, 108016. DOI

Ngezimana, W., & Agenbag, G.A. (2013). Effects of nitrogen (N) and sulphur (S) on canola (Brassica napus L.) vegetative and reproductive growth under controlled conditions. African Journal of Agricultural, 8(39), 4887-4894. PDF

Omondi, J. O., Lazarovitch, N., Rachmilevitch, S., Yermiyahu, U., & Sperling, O. (2019). High nitrogen availability limits photosynthesis and compromises carbohydrate allocation to storage in roots of Manihot esculenta crantz. Frontiers in Plant Science, 10, 1041. DOI

Oteino, N., Lally, R. D., Kiwanuka, S., Lloyd, A., Ryan, D., Germaine, K. J., & Dowling, D. N. (2015). Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates. Frontiers in Microbiology, 6, 745. DOI

Pan, K., Lu, C., Nie, P., Hu, M., Zhou, X., Chen, X., & Wang, W. (2021). Predominantly symplastic phloem unloading of photosynthates maintains efficient starch accumulation in the cassava storage roots (Manihot esculenta Crantz). BMC Plant Biology, 21(1), 318. DOI

Pathak, V. M., Verma, V. K., Rawat, B. S., Kaur, B., Babu, N., Sharma, A., Dewali, S., Yadav, M., Kumari, R., Singh, S., Mohapatra, A., Pandey, V., Rana, N., & Cunill, J. M. (2022). Current status of pesticide effects on environment, human health and it's eco-friendly management as bioremediation: A comprehensive review. Frontiers in microbiology, 13, 962619. DOI

Perchlik, M., & Tegeder, M. (2017). Improving plant nitrogen use efficiency through alteration of amino acid transport processes. Plant physiology, 175(1), 235–247. DOI

Perdanatika, A., Suntoro, & Pardjanto. (2018). The effects of rice husk ash and dolomite on soybean yield at latosol soil. SAINS TANAH-Journal of Soil Science and Agriclimatology, 15(1), 29-34. DOI

Purwani, J., & Ginting, R. C. B. (2023). Responses of soybean with a biofertilizer contains Ochrobactrum sp., Alcaligenes sp., and Bacillus sp. and the various doses of NPK fertilizer in Inceptisols, West Java. IOP Conference Series: Earth and Environmental Science 1253(1), 012045. DOI

Raimi, A., Roopnarain, A., & Adeleke, R. (2021). Biofertilizer production in Africa: Current status, factors impeding adoption and strategies for success. Scientific African, 11. DOI

Rajiman, R. (2017). Pengaruh takaran pupuk hayati dan dosis pupuk anorganik terhadap hasil jagung. Prosiding Seminar Nasional Fakultas Pertanian UNS, 1(1), 114-118.

Ratnasari, D., Bangun, M. K., & Damanik, R. I. M. (2015). Respons dua varietas kedelai (Glycine max (L.) Merrill.) pada pemberian pupuk hayati dan NPK majemuk. Jurnal Online Agroekoteknologi, 3(1), 276 - 282. website

Salsabila, S., Rahmawati, H. N., & Fatimah. (2022). Screening and identification of free nitrogen-fixing bacteria from rhizosphere of mangrove Jenu, Tuban. Jurnal of Bio-Molecule Research and Engineering, 1(2), 55-65. website

Sefrila, M., Ghulamahdi, M., Purwono, Melati, M., & Mansur, I. (2023). Growth and production of soybean on different inoculant sources of arbuscular mycorrhizal fungi and water saturation periods. Indonesian Journal of Agronomy, 51(1), 45-53. DOI

Sharma, B., Yadav, L., Pandey, M., & Shrestha, J. (2022). Application of biofertilizers in crop production: A review. Peruvian Journal of Agronomy, 6(1), 13–31. DOI

Sumajow, A. Y. M., Rogi, J. E. X., & Tumbeleka, S. (2016). Pengaruh pemangkasan daun bagian bawah terhadap produksi jagung manis (Zea mays var. saccharata Sturt). Agri-Sosioekonomi: Jurnal Ilmiah Sosial Ekonomi Pertanian, 12(1A), 67-72. DOI

Takate, B. D., & Gaykar, B. M. (2021). Effect of different concentrations of glycerol on survival of Azotobacter chroococcum local strains isolated from wild grasses. Asian Journal of Applied Science and Technology (AJAST), 5(4), 16-23. DOI

Tambunan, S. B., Afkar, & Sebayang, N. S. (2019). Growth and yields response of some varieties of soybean (Glycine Max (L) Merill) on Ultisol soil. Indonesian Journal of Agricultural Research, 2(3), 196– 204. DOI

Umadi, S. S., Ilyas, S., & Widyastuti, R. (2023). Characteristic and viability of nitrogen fixation bacteria and phosphate solubilizing bacteria in biochar carrier media. Jurnal Ilmu Tanah Dan Lingkungan, 25(2), 40-45. DOI

Wang, R., Bicharanloo, B., Hou, E., Jiang, Y., & Dijkstra, F. A. (2022). Phosphorus supply increases nitrogen transformation rates and retention in soil: A global meta-analysis. Earth's Future, 10, e2021EF002479. DOI

Widodo, K. H., & Kusuma, Z. (2018). Effects of compost on soul physical properties and growth of maize on an Inceptisol. Jurnal Tanah dan Sumberdaya Lahan, 5(2), 959-967. website

Win, M., Nakasathien, S., & Sarobol, E. (2010). Effects of phosphorus on seed oil and protein contents and phosphorus use efficiency in some soybean varieties. Kasetsart Journal-Natural Science, 44(1), 1-9.

Zhu, J., Wang, X., Jin, X., Jiang, L., Lin, H., Hu, Y., Liu, J., & He, Z. (2024). Relative position of seeds driven the seedling growth are mediated by root-leaf traits. Journal of Plant Ecology, 17(2), rtae004. DOI




DOI: http://doi.org/10.17503/agrivita.v47i1.4672

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