Apirat Bundit, Anyamanee Auvuchanon, Tosapon Pornprom


Biodiversity of Rottboellia cochinchinensis from different areas were studied by morphological traits and amplified fragment length polymorphism (AFLP) analysis for classify of an allelopathic ability. The correlation of the similarity/distance between AFLP markers (Jaccard coefficient) and morphological traits (Euclidean distance) was significant with r = − 0.84**. Itchgrass could be divided into two groups from both UPGMA and STRUCTURE analyses: the group A consisted of itchgrass from Chaehom-Lampang, Si Thep-Phetchabun, Phrom Phiram-Phitsanulok, Amphur Muang-Nakhon Sawan, Kamalasai-Kalasin, Amphur Muang-Chachoengsao and Bang Yai-Nonthaburi, whereas itchgrass from Amphur Muang-Chiang Mai, Pak Chong-Nakhon Ratchasima and Kamphaeng Saen-Nakhon Pathom constituted the group B. Allelopathic properties of itchgrass as representative from different group were determined in bioassay test, the result showed that the aqueous extract of itchgrass from Chaehom-Lampang area has a strong allelopathic ability on growth of Echinochloa crus-galli L., Bidens pilosa L. and Lactuca sativa L. than the other group. The molecular analysis was strongly supported in morphological analysis clustering with bioassay test for allelopathic ability, specific morphological traits were soft trichomes, and the dark purple stems and roots which can be used for the preliminary classification of allelopathic ability. Our findings suggest that classification of itchgrass by morphological traits is related to the analysis of the genetic relationship of itchgrass with AFLP analysis, that allowing the assessment of the bio-diversity of itchgrass and their allelopathic potentials.

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Alloub, H., A.S. Juraimi, A. Rajan, J. Kadir, M.S. Saad and S. Sastroutomo. 2005. Growth behavior of itchgrass (Rottboellia cochinchinensis) in Peninsular Malaysia. Weed Biology and Management 5:8-13.

Alves, P.L.C.A., M.F. Bachega, J.R. Moro, M.V.F. Lemos, E.C.C. Alves, M.A.S. Silva and F.V. Moro. 2003. Identification and characterization of different accessions of itchgrass (Rottboellia cochinchinensis). Weed Science 51:177-180.

Cullings, K.W. 1992. Design and testing of a plant-specific PCR primer for ecological and evolutionary studies. Molecular Ecology 1:233-240.

Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11-15.

Eivazi, A.R., M.R. Naghavi, M. Hajheidari, S.M. Pirseyedi, M.R. Ghaffari, S.A. Mohammadi, I. Majidi, G.H. Salekdeh and M. Mardi. 2007. Assessing wheat (Triticum aestivum L.) genetic diversity using quality traits, amplified fragment length polymorphisms, simple sequence repeats and proteome analysis. Annals of Applied Biology 152:81-91.

Falush, D., M. Stephens and J.K. Pritchard. 2003. Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164:1567-1587.

Jaccard, P. 1908. Nouvelles recherches sur la distribution florale. Bulletin de la Societe Vaudoise des Sciences Naturelles 44:223-270.

Kantartzi, S.K. and J.M. Stewart. 2008. Association analysis of fibre traits in Gossypium arboreum accessions. Plant Breeding 127:173-179.

Kato-Noguchi, H., S. Kosemura, S. Yamamura, J. Mizutani and K. Hasegawa. 1994. Allelopathy of oats: I. Assessment of allelopathic potential of extract of oat shoots and identification of an allelochemical. Journal of Chemical Ecology 20:309-314.

Kobayashi, K., D. Itaya, P. Mahatamnuchoke and T. Pornprom. 2008. Allelopathic potential of itchgrass (Rottboellia exaltata L. f.) powder incorporated into soil. Weed Biology and Management 8:64-68.

Maccaferri, M., S. Corinna, E. Noli and R. Tuberosa. 2005. Population structure and long-range linkage disequilibrium in a durum wheat elite collection. Molecular Breeding 15:271-289.

Mantel, N.A. 1967. The detection of disease clustering and a generalized regression approach. Cancer Research 27:209-220.

Meksawat, S. and T. Pornprom. 2010. Allelopathic effect of itchgrass (Rottboellia cochinchinensis (Lour.) W.D. Clayton) on seed germination and plant growth. Weed Biology and Management 10:16-24.

Millhollon, R.W. and D.M. Burner. 1993. Itchgrass (Rottboellia exaltata) biotypes in world populations. Weed Science 41:379-387.

Na, H.R., C. Kim and H.K. Choi. 2010. Genetic relationship and genetic diversity among Typha taxa from East Asia based on AFLP markers. Aquatic Botany 92:207-213.

Ohno, S., K. Tomita-Yokotani, S. Kosemura, M. Node, T. Suzuki, M. Amano, K. Yasui, T. Goto, S. Yamamura and K. Hasegawa. 2001. A species-selective allelopathic substance from germinating sunflower (Helianthus annuus L.) seeds. Phytochemistry 56:577-581.

Powell, W., M. Morgante and C. Andre. 1996. The comparison of RFLP, RADP, AFLP, and SSR (microsatellite) markers for germoplasm analysis. Molecular Breeding 2:225-238.

Pritchard, J.K., M. Stephens and P. Donnelly. 2000. Inference of population structure using multilocus genotype data. Genetics 155:945-959.

Rohlf, F.J. 1998. NTSYS-pc: numerical taxonomy and multivariate analysis system. Version 2.0, user’s guide, New York, USA.

Skot, L., M.O. Humphreys, I. Armstead, S. Heywood, K.P. Skot, R. Sanderson, I.D. Thomas, K.H. Chorlton and N.R.S. Hamilton. 2005. An association mapping approach to identify flowering time genes in natural populations of Lolium perenne (L.). Molecular Breeding 15:233-245.

Tabacchi, M., R. Mantegazza, A. Spada and A. Ferrero. 2006. Morphological traits and molecular markers for classification of Echinochloa species from Italian rice fields. Weed Science 54:1086-1093.

Tara Satyavathi, C., K.V. Bhat, C. Bharadwaj, S.P. Tiwari and V.K. Chaudhury. 2006. AFLP analysis of genetic diversity in Indian soybean [Glycine max (L.) Merr.] varieties. Genetic Resources and Crop Evolution 53:1069-1079.

Vos, P., R. Hogers and M. Bleeker. 1995. AFLP a new technique for DNA fingerprinting. Nucleic Acids Research 23:4407-4414.

Weston, L.A., I.S. Alsaadawi and S.R. Baerson. 2013. Sorghum Allelopathy-From Ecosystem to Molecule. Journal of Chemical Ecology 39:142-153.

Zhao, J., M.J. Paulo, D. Jamar, P. Lou, F. Eeuwijk, G. Bonnema, D. Vreugdenhil and M. Koornneef. 2007. Association mapping of leaf traits, flowering time, and phytate content in Brassica rapa. Genome 50:963-973.

Zimdahl, R.L. 1993. Weed ecology, in Fundamentals of Weed Science. Academic Press, New York, USA.

Zuo, S.P., Y.Q. Ma and S. Inanaga. 2007. Allelopathy variation in dryland winter wheat (Triticum aestivum L.) accessions grown on the Loess Plateau of China for about fifty years. Genetic Resources and Crop Evolution 54:1381-1393.


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