EXOGENOUS APPLICATION OF TRYPTOPHAN AND INDOLE ACETIC ACID (IAA) TO INDUCE ROOT NODULE FORMATION AND INCREASE SOYBEAN YIELD IN ACID, NEUTRAL AND ALKALINE SOIL
Abstract
The research aimed to study whether soil pH affects exogenous application of amino acid tryptophan and IAA in increasing root nodules and soybean yield. The experiment was conducted in greenhouse using 20 cm diameter plastic pot filled with fine soil, arranged in completely randomized design (CRD) with four treatments. Each treatment combination was repeated three times. Three seeds of local soybean variety were planted into each pot then to remain one plant a week after planting. Variables observed were root nodules number, shoot dry weight and seed yield. Plant nutrients were supplied in the form of solution to meet crop needs. The data obtained were analyzed statistically by F test at 5% of level confidence, followed by Duncan's multiple range test when there was a significant effect. The results showed that both exogenous amino acid tryptophan and IAA increased number of root nodules, shoot and root dry weight and soybean yield. Higher root nodules number was taken from the treatment combination of 0.001 ppm IAA applied at V3 in Alfisol and Vertisols, and 1.0 ppm on Entisols. However, the highest soybean yields were taken from the treatment combination of 1.0 ppm tryptophan applied at V0 in Alfisols (6.51 g plant-1).
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Afza, R., G. Hardarson and F. Zapata. 1987. Effect of delayed soil and foliar N fertilization on yield and N2 fixation of soybean. Plant and Soil 97 : 361 – 368.
Anonymous. 1986. Legum inoculant for Soybean (in Indonesian). (Rhizobium inoculum for soybeans). Leaflet. Laboratory of Microbiology, Fac.of. Agriculture, Gadjah Mada Univ., Yogyakarta
Baca, B. and C. Elmerich. 2007. Microbial production of plant hormones, in: C. Elmerich, W.E. Newton (Eds.), Associative and Endophytic Nitrogen-Fixing Bacteria and Cyanobacterial Associations. Springer. The Netherland. pp. 113-143.
Bianco, C., B. Senatore, S. Arbucci, G. Pieraccini and R. Defez. 2014. Modulation of Endogenous Indole-3-Acetic Acid Biosynthesis in Bacteroids within Medicago sativa Nodules. Appl. Environ. Microbiol. July 2014. 80 (14): 4286-4293.
Coyne, M.S. 1999. Soil Microbiology : An Exploratory Approach. Chapter 22. The Nitrogen Cycle - Nitrogen Fixation. Delmar Publishers. an International Thomson Publishing company. Albany. p. 279 - 290.
Elmerich, C. and W.E. Newton. 2007. Associative and Endophytic Nitrogen-fixing Bacteria and Cyanobacterial Associations. Springer, Dordrecht, The Netherlands.
Ghosh, A. and Basu, B.. 2002. Equivalent viscous damping of a liquid column damper. Proc. VETOMAC-II (Second International Conference on Vibration Engineering and Technology of Machinery). BARC. Mumbai. India. P. 104 - 105.
Graham, P. H. 2008. Chapter 2. Ecology of The Root-Nodule Bacteria of Legumes. In : Dilworth, M.J., E.K. James, J.I. Sprent and W.E. Newton., Nitrogen-fixing Leguminous Symbioses. Springer Science+Business Media B.V. p. 23–58.
Gray, T.R.G. and S.T. William. 1971. Soil Micro-Organisms. Longman. London.
Guilfoyle TJ,. Ulmasov T and Hagen G.. 1998. The ARF family of transcription factors and their role in plant hormone-responsive transcription. Cell Mol Life Sci 54: 619–627.
Hidayat, O.O. 1985. The morphology of soybean plants.(in Indonesian). in : Somaatmadja, S., M. Ismunadji, Sumarno, M. Syams, S.O. Manurung and Yuswandi.., Soybean. PPPTP. Bogor. p. 73 – 86
Howieson, J. G., R. J. Yates, K. J. Foster, D. Real,And R. B. Besier. 2008. Chapter 12. Prospects For The Future Use Of Legumes. In : Dilworth, M.J., E.K. James, J.I. Sprent and W.E. Newton., Nitrogen-fixing Leguminous Symbioses. Springer Science+Business Media B.V. p. 363–393.
Ismunadji, M. and S.N. Mahmud. 1985. The role of micro nutrients to increase soybean production. (in Indonesian). in : Somaat-madja, S., M. Ismunadji, Sumarno, M. Syams, S.O. Manurung and Yuswandi. PPPTP. Bogor. p. 189 - 215.
Jutono. 1985. Rhizobium Inoculation (in Indonesian). In : Somaatmadja, S., M. Ismunadji, Sumarno, M. Syams, S.O. Manurung & Yuswandi.., Soybean. PPPTP. Bogor. p. 216 – 230. Kamal, M. 2000. Response of nodule formation, N2 fixation and sugar content in soybean nodule to epi-brassinolide application. Journal Agrista 4 (2) : 120 - 126.
Keyser, H.H., P. Somasegaran and B.B. Bohlool. 1993. Rhizobial Ecology and Technology. In.F.B. Metting, Jr., Soil Microbial Ecology. Application in Agricultural and Environmental Management. Marcel Dekker Inc. New York. p. 579 - 594.
Mishra, S.N., P.K. Jaiwal, R.P. Singh and H.S. Srivastava. 1999. Rhizobium – Legume Association. In : Srivastava, H.S. and R.P. Singh (edt), Nitrogen Nutrition and Plant Growth. Science Publisher Inc. USA. p. 45 - 102.
Nonhebel H.M. and Bandurski R.S.. 1984. Oxidation of indole-3-acetic acid and oxindole-3-acetic acid to 2,3-dihydro-7-hydroxy-2-oxo-1H indole-3-acetic acid-7'-O-beta-D glucopyranoside in Zea mays seedlings. Plant Physiol 76: 979-983.
Ohyama, T., N. Ohtake, K. Sueyoshi, K. Tewari, Y. Takahashi, S. Ito, T. Nishiwaki, Y. Nagumo, S. Ishii and T. Sato. 2009. Nitrogen Fixation and Metabolism in Soybean Plants. Chapter 1. Introduction. Nova Science Publishers, Inc. New York. p. 1 - 12.
Pandey, J., D. Sinha, D. And D.P.S. Bhawuk, (Eds.). 1996. Asian contributions to cross-cultural psychology. New Delhi: Sage Publication
Pasaribu, D. and S.Suprapto S. 1985. NPK Fertili-zation on Soybean. (in Indonesian) in Somaatmadja, S., M. Ismunadji, Sumarno, M. Syams, S.O. Manurung and Yuswandi. PPPTP. Bogor. p. 159 - 169. Paul, E.A. and. F.E. Clark. 1989. Soil Microbiology and Biochemestry. Acad. Press Inc. London. p. 164 – 197.
Prieto C., E., Cordoba C., N.M., Montenegro J., A.M. and G.E. González-Mariño,. 2011. Production of Indole-3-Acetic Acid in the Culture Medium of Microalga Scene-desmus obliquus (UTEX 393). J. Braz. Chem. Soc., Vol. 22, No. 12, 2355-2361
Richardson, A.E., M.A. Djordjevic, B.G. Rolfe and R.J. Simpson. 1988. Effect of pH, Ca, and Al on the exudation from clover seedlings of coumpouns that induce the expression of nodulation genes in Rhizobium trifolii. Plant and Soil 109 : 37 – 47.
Spaepen, S. and J. Vanderleyden. 2010 Auxin and Plant-Microbe Interactions. Cold Spring Harb Perspect Biol doi: 10.1101/cshperspect.a001438 published online November 17, 2010.
Sudadi, 2011. Exogenous application of tryptophan and indole acetic acid (IAA) to induce root nodule formation and increase yield of soybean. Agricultural Science Research Journal. 2(4) : 134 – 139, April
Widjaja-Adhi, IPG. 1985. Liming of acid soil for soybean. (in Indonesian). In : Soma-atmadja, S., M. Ismunadji, Sumarno, M. Syams, S.O. Manurung and Yuswandi. PPPTP. Bogor. p. 171 - 188.
Zuberer, D.A. 1999. Biological Dinitrogen Fixation. : Introduction and Nonsymbiotic. In : Silvia, D.M., J.J. Fuhrmann, P.G. Hartel and D.A. Zuberer (edt)., Principles and Application of Soil Microbiology. Prentice Hall. New Jersey. p. 297 – 321.
DOI: http://doi.org/10.17503/agrivita.v37i1.444
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