DISSECTING QUANTITATIVE TRAIT LOCI FOR AGRONOMIC TRAITS RESPONDING TO IRON DEFICEINCY IN MUNGBEAN [Vigna radiata (L.) Wilczek]

Prakit Somta, Prayoon Prathet, Alisa Kongjaimun, Peerasak Srinives

Abstract


Calcareous soil is prevalent in many areas of the world agricultural land causing substantial yield loss of crops. We previously identified two quantitative trait locus (QTL) qIDC3.1 and qIDC2.1 controlling leaf chlorosis in mungbean grown in calcareous soil in two years (2010 and 2011) using visual score and SPAD measurement in a RIL population derived from KPS2 (susceptible) and NM10-12-1 (resistant). The two QTLs together accounted for 50% of the total leaf chlorosis variation and only qIDC3.1 was confirmed, although heritability estimated for the traits was as high as 91.96%. In this study, we detected QTLs associated with days to flowering , plant height, number of pods per plants, number of seeds per pods, and seed yield per plants in the same population grown under the same environment with the aim to identify additional QTLs controlling resistance to calcareous soil in mungbean. Single marker analysis revealed 18 simple sequence repeat markers, while composite interval mapping identified 33 QTLs on six linkage groups (1A, 2, 3, 4, 5 and 9) controlling the five agronomic traits. QTL cluster on LG 3 coincided with the position of qIDC3.1, while QTL cluster on LG 2 was not far from qIDC2.1. The results confirmed the importance of qIDC3.1 and qIDC2.1 and revealed four new QTLs for the resistance to calcareous soil. 


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References


Blair, M.W., F. Pedraza, H.F. Buendia, E. Gaitán-Solís, S.E. Beebe, P. Gepts and J. Thome. 2003. Development of a genome-wide anchored microsatellite map of common bean (Phaseolus vulgaris L.). Theor. Appl. Genet. 107:1362–1374.

Buso, G.S.C., Z.P.S. Amaral, R.P.V. Brondani and M.E. Ferreira. 2006. Microsatellite markers for the common bean Phaseolus vulgaris. Mol. Ecol. Notes. 6:252–254.

Chankaew, S., P. Somta, W. Sorajjapinun and P. Srinives. 2011. Quantitative trait loci mapping of Cercospora leaf spot resistance in mungbean, Vigna radiata (L.) Wilczek. Mol. Breed. 28:255–264.

Guerra-Sanz, J.M. 2004. New SSR markers of Phaseolus vulgaris from sequence databases. Plant Breed. 123:87–89.

Isemura, T., A. Kaga, S. Tabata, P. Somta, P. Srinives, T. Shimizu, U. Jo, D.A. Vaughan and N. Tomooka. 2012. Construction of a genetic linkage map and genetic analysis of domestication related traits in mungbean (Vigna radiata). PLoS ONE 7(8): e41304. doi: 10.1371/journal.pone.0041304.

Kajonphol, T., C. Sangsiri, P. Somta, T. Toojinda and P. Srinives. 2012. SSR map construction and quantitative trait loci (QTL) identification of major agronomic traits in mungbean (Vigna radiata (L.) Wilczek). SABRAO J. Breed. Genet. 44:71–86.

Kitsanachandee, R., P. Somta, O. Chatchawankanphanich, K.P. Akhtar, T.M. Shah, R.M. Nair, T.S. Bains, A. Sirari, L. Kaur and P. Srinives. 2013. Detection of quantitative trait loci for mungbean yellow mosaic India virus (MYMIV) resistance in mungbean (Vigna radiata (L.) Wilczek) in India and Pakistan. Breed. Sci. 63:367–373.

Kongjaimun, A., A. Kaga, N. Tomooka, P. Somta, T. Shimizu, Y. Shu, T. Isemura, D.A. Vaughan and P. Srinives. 2012. An SSR-based linkage map of yardlong bean (Vigna unguiculata (L.) Walp. subsp. unguiculata Sesquipedalis Group) and QTL analysis of pod length. Genome 55:81–92.

Kosambi, D.D. 1944. The estimation of map distances from recombination values. Ann. Eugen. 12:172–175.

Li, C.D., C.A. Fatokun, B. Ubi, B.B. Singh and G.J. Scoles. 2001. Determining genetic similarities and relationships among cowpea breeding lines and cultivars by microsatellite markers. Crop Sci. 41:189–197.

Lin, S., S. Cianzio and R. Shoemaker. 1997. Mapping genetics loci for iron deficiency chlorosis in soybean. Mol. Breed. 3:219–229.

Lin, S., S. Cianzio and R. Shoemaker. 2000. Molecular characterization of iron deficiency chlorosis in soybean. J. Plant Nutr. 23:1929–1939.

Lodhi, M.A., G.N. Ye, N.F. Weeden and B.I. Reisch. 1994. A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol. Biol. Rep. 12:6–13

Nopparat, S., P. Srinives, R. Kaveeta and S. Jintakanon. 1997. An inheritance of mungbean tolerance to microessential element deficiency in Takhli soil series. P. 78–82. In Romkaew J. et al. (Editors) Proceedings of National Mungbean Research Conference VII, Phitsanulok, Thailand.

Park, H.G. 1978. Suggested cultural practices for mungbean. Asian Vegetable Research and Development Center, Tainan, Taiwan.

Prathet, P., P. Somta and P. Srinives. 2012. Mapping QTL conferring resistance to iron deficiency chlorosis in mungbean [Vigna radiata (L.) Wilczek]. Field Crops Res 137:230–236.

Quarrie, S., S.P. Quarrie, R. Radosevic, D. Rancic, A. Kaminska, J.D. Barnes, M. Leverington, C. Ceoloni and D. Dodig. 2006. Dissecting a wheat QTL for yield present in a range of environments: from the QTL to candidate genes. J. Exp. Bot. 57: 2627–2637

R Development Core Team. 2010. R: A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna, Austria.

Seehalak, W., P. Somta, W. Musch and P. Srinives. 2009. Microsatellite markers for mungbean developed from sequence database. Mol. Ecol. Resour. 9:862–864.

Shi, T., R. Li, Z. Zhao, G. Ding, Y. Long, J. Meng, F. Xu and L. Shi. 2013. QTL for yield traits and their association with functional genes in response to phosphorus deficiency in Brassica napus. PLoS ONE 8(1): e54559. doi: 10.1371/journal.pone.0054559

Somta, P., W. Musch, B. Kongsamai, S. Chanprame, S. Nakasathien, T. Toojinda, W. Sorajjapinun, W. Seehaluk, S. Tragoonrung and P. Srinives. 2008. New microsatellite

markers isolated from mungbean (Vigna radiata (L.) Wilczek). Mol. Ecol. Resour. 8:1155–1157

Somta, P., W. Seehalak and P. Srinives. 2009. Development, characterization and cross-species amplification of mungbean (Vigna radiata) genic microsatellite markers. Conserv. Genet. 10:1939–1943.

Tangphatsornruang, S., P. Somta, P. Uthaipaisanwong, J. Chanprasert, D. Sangsrakru, W. Seehalak, W. Sommanas, S. Tragoonrung and P. Srinives. 2009. Characterization of microsatellites and gene contents from genome shotgun sequences of mungbean (Vigna radiata (L.) Wilczek). BMC Plant Biol. 9:137. doi:10.1186/1471-2229-9-137

Wang, S., C.J. Basten and Z.B., Zeng. 2012. Window QTL Cartographer 2.5. Department of Statistics, North Carolina State University, Raleigh, NC, USA.

Wang, X.W., A. Kaga, N. Tomooka, D.A. Vaughan. 2004. The development of SSR markers by a new method in plants and their application to gene flow studies in azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi]. Theor. Appl. Genet. 109:352–360.

Zhao Z., Wu, L., Nian, F., Ding, G., Shi, T., et al. 2012 Dissecting quantitative trait loci for boron efficiency across multiple environments in Brassica napus. PLoS ONE 7(9): e45215. doi:10.1371/journal.pone.0045215




DOI: http://doi.org/10.17503/agrivita.v36i2.391

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