Screening of Plant Growth-Promoting Halotolerant Bacteria Isolated from Weeds Rhizosphere Grown in Saline Soil

Luqman Qurata Aini, Nurul Aini, Wiwin Sumiya Dwi Yamika, Adi Setiawan

Abstract


This study aimed to screen and characterize halotolerant bacterial isolates, which could enhance plant growth performance in saline soil. Halotolerant bacteria was isolated from weeds rhizosphere grown in saline soil of coastal agricultural land located at Brondong District, Lamongan Regency, East Java Province, Indonesia. This research was conducted from June to September 2018. Seven bacterial isolates can grow in a Nutrient Agar medium containing 10% of NaCl, suggesting that these bacteria were halotolerant. Furthermore, all bacterial isolates were shown to produce indol acetic acid (IAA) and do not induce a hypersensitive response when infiltrated into tobacco leaves. These results explain that these bacteria had potency as plant growth-promoting rhizobacteria (PGPR) and were not tend to be the plant pathogen. The growth of seedlings when inoculated in cucumber seed grown in saline media were higher than those in control. This result suggests that the halotolerant bacteria can enhance the development of cucumber seedlings in saline stress conditions. Three potential halotolerant bacteria i.e., SN22, SN23, SN26 were selected and molecularly identified as Bacillus megaterium, Bacillus sp., and B. megaterium, respectively.


Keywords


Bacillus; Halotolerant Bacteria; PGPR; Saline Soil

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References


Abeer, H., Abd_Allah, E. F., Alqarawi, A. A., Al-Huqail, A. A., Alshalawi, S. R. M., Wirth, S., & Dilfuza, E. (2015). Impact of plant growth promoting Bacillus subtilis on growth and physiological parameters of Bassia indica (Indian bassia) grown udder salt stress. Pakistan Journal of Botany, 47(5), 1735–1741. Retrieved from PDF

Albdaiwi, R. N., Khyami-Horani, H., Ayad, J. Y., Alananbeh, K. M., & Al-Sayaydeh, R. (2019). Isolation and characterization of halotolerant plant growth promoting rhizobacteria from durum wheat (Triticum turgidum subsp. durum) cultivated in saline areas of the dead sea region. Frontiers in Microbiology, 10, 01639. DOI

Ansari, M., Shekari, F., Mohammadi, M. H., Biró, B., & Végvári, G. (2017). Improving germination indices of alfalfa cultivars under saline stress by inoculation with beneficial bacteria. Seed Science and Technology, 45(2), 475–484. DOI

Arifin, I., Herawati, A., Mujiyo, S., & Widijanto, H. (2021). Semar Sandy-app : monitoring system for sandy soil irrigation based on android application. IOP Conference Series: Earth and Environmental Science, 637, 012002. DOI

Baghbani, A., Forghani, A. H., & Kadkhodaie, A. (2013). Study of salinity stress on germination and seedling growth in greenhouse cucumber cultivars. Journal of Basic and Applied Scientific Research, 3(3), 1137–1140. Retrieved from PDF

Berrada, I., Willems, A., De Vos, P., El fahime, El M., Swings, J., Bendaou, N., … Amar, M. (2012). Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco). African Journal of Microbiology Research, 6(10), 2419-2434. DOI

Chaiharn, M., & Lumyong, S. (2011). Screening and optimization of indole-3-acetic acid production and phosphate solubilization from rhizobacteria aimed at improving plant growth. Current Microbiology, 62(1), 173–181. DOI

Dawwam, G. E., Elbeltagy, A., Emara, H. M., Abbas, I. H., & Hassan, M. M. (2013). Beneficial effect of plant growth promoting bacteria isolated from the roots of potato plant. Annals of Agricultural Sciences, 58(2), 195–201. DOI

de Souza, R., Ambrosini, A., & Passaglia, L. M. P. (2015). Plant growth-promoting bacteria as inoculants in agricultural soils. Genetics and Molecular Biology, 38(4), 401–419. DOI

Dogan, M., Tipirdamaz, R., & Demir, Y. (2010). Salt resistance of tomato species grown in sand culture. Plant, Soil and Environment, 56(11), 499–507. DOI

Egamberdieva, D., Shurigin, V., Gopalakrishnan, S., & Sharma, R. (2014). Growth and symbiotic performance of chickpea (Cicer arietinum) cultivars under saline soil conditions. Journal of Biological and Chemical Research, 31(1), 333–341. Retrieved from DOI

Egamberdieva, D., Wirth, S. J., Alqarawi, A. A., Abd_Allah, E. F., & Hashem, A. (2017). Phytohormones and beneficial microbes: essential components for plants to balance stress and fitness. Frontiers in Microbiology, 8, 2104. DOI

Etesami, H., & Beattie, G. A. (2018). Mining halophytes for plant growth-promoting halotolerant bacteria to enhance the salinity tolerance of non-halophytic crops. Frontiers in Microbiology, 9, 148. DOI

Farace, G., Fernandez, O., Jacquens, L., Coutte, F., Krier, F., Jacques, P., … Dorey, S. (2015). Cyclic lipopeptides from Bacillus subtilis activate distinct patterns of defence responses in grapevine. Molecular Plant Pathology, 16(2), 177–187. DOI

Ferreira, N. C., de Cassia Lima Mazzuchelli, R., Pacheco, A. C., de Araujo, F. F., Antunes, J. E. L., & de Araujo, A. S. F. (2018). Bacillus subtilis improves maize tolerance to salinity. Ciencia Rural, 48(8), 6–9. DOI

Gopalakrishnan, T., Hasan, Md K., Sanaul Haque, A. T. M., Jayasinghe, S. L., & Kumar, L. (2019). Sustainability of coastal agriculture under climate change. Sustainability, 11(24), 7200. DOI

Hahm, M.-S., Son, J.-S., Hwang, Y.-J., Kwon, D.-K., & Ghim, S.-Y. (2017). Alleviation of salt stress in pepper (Capsicum annum L.) plants by plant growth-promoting rhizobacteria. Journal of Microbiology and Biotechnology, 27(10), 1790–1797. DOI

Hoffmann, J., Berni, R., Hausman, J.-F., & Guerriero, G. (2020). A review on the beneficial role of silicon against salinity in non-accumulator crops: Tomato as a model. Biomolecules, 10(9), 1284. DOI

Hussain, S., Zhang, J.-H., Zhong, C., Zhu, L.-F., Cao, X.-C., Yu, S.-M., … Jin, Q.-Y. (2017). Effects of salt stress on rice growth, development characteristics, and the regulating ways: A review. Journal of Integrative Agriculture, 16(11), 2357–2374. DOI

Iqbal, M. A., Khalid, M., Zahir, Z. A., & Ahmad, R. (2016). Auxin producing plant growth promoting rhizobacteria improve growth, physiology and yield of maize under saline field conditions. International Journal of Agriculture and Biology, 18(1), 37–45. DOI

Islam, F., Yasmeen, T., Arif, M. S., Ali, S., Ali, B., Hameed, S., & Zhou, W. (2016). Plant growth promoting bacteria confer salt tolerance in Vigna radiata by up-regulating antioxidant defense and biological soil fertility. Plant Growth Regulation, 80(1), 23–36. DOI

Jha, Y., & Subramanian, R. B. (2014). PGPR regulate caspase-like activity, programmed cell death, and antioxidant enzyme activity in paddy under salinity. Physiology and Molecular Biology of Plants, 20(2), 201–207. DOI

Jha, Y., Subramanian, R. B., & Patel, S. (2011). Combination of endophytic and rhizospheric plant growth promoting rhizobacteria in Oryza sativa shows higher accumulation of osmoprotectant against saline stress. Acta Physiologiae Plantarum, 33(3), 797–802. DOI

Ji, C., Tian, H., Wang, X., Song, X., Ju, R., Li, H., … Liu, X. (2022). Bacillus subtilis HG-15, a halotolerant rhizoplane bacterium, promotes growth and salinity tolerance in wheat (Triticum aestivum). BioMed Research International, 2022, 9506227. DOI

Kumar, S., Stecher, G., & Tamura, K. (2016). MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution, 33(7), 1870–1874. DOI

Mike-Anosike, E. E., Braide, W., & Adeleye, S. A. (2018). Studies on Indole Acetic Acid (IAA) production by rhizobacteria and growth promoting potentials. International Journal of Advanced Research in Biological Sciences, 5(2), 133–140. Retrieved from PDF

Mohite, B. (2013). Isolation and characterization of indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. Journal of Soil Science and Plant Nutrition, 13(3), 638–649. DOI

Munif, A., Hallmann, J., & Sikora, R. A. (2013). The influence of endophytic bacteria on Meloidogyne incognita infection and tomato plant growth. Journal of the International Society for Southeast Asian Agricultural Sciences, 19(2), 68–74. Retrieved from PDF

Nadeem, S. M., Ahmad, M., Naveed, M., Imran, M., Zahir, Z. A., & Crowley, D. E. (2016). Relationship between in vitro characterization and comparative efficacy of plant growth-promoting rhizobacteria for improving cucumber salt tolerance. Archives of Microbiology, 198(4), 379–387. DOI

Nosheen, S., Ajmal, I., & Song, Y. (2021). Microbes as biofertilizers, a potential approach for sustainable crop production. Sustainability, 13(4), 1868. DOI

Obeidat, M. (2017). Isolation and characterization of extremely halotolerant Bacillus species from Dead Sea black mud and determination of their antimicrobial and hydrolytic activities. African Journal of Microbiology Research, 11(32), 1303–1314. DOI

Orhan, F. (2016). Alleviation of salt stress by halotolerant and halophilic plant growth-promoting bacteria in wheat (Triticum aestivum). Brazilian Journal of Microbiology, 47(3), 621–627. DOI

Ortiz, A., & Sansinenea, E. (2022). The role of beneficial microorganisms in soil quality and plant health. Sustainability, 14(9), 5358. DOI

Paul, D., & Lade, H. (2014). Plant-growth-promoting rhizobacteria to improve crop growth in saline soils: a review. Agronomy for Sustainable Development, 34(4), 737–752. DOI

Ramadoss, D., Lakkineni, V. K., Bose, P., Ali, S., & Annapurna, K. (2013). Mitigation of salt stress in wheat seedlings by halotolerant bacteria isolated from saline habitats. SpringerPlus, 2(1), 6. DOI

Reddy, I. N. B. L., Kim, B.-K., Yoon, I.-S., Kim, K.-H., & Kwon, T.-R. (2017). Salt tolerance in rice: Focus on mechanisms and approaches. Rice Science, 24(3), 123–144. DOI

Ruginescu, R., Gomoiu, I., Popescu, O., Cojoc, R., Neagu, S., Lucaci, I., … Enache, M. (2020). Bioprospecting for novel halophilic and halotolerant sources of hydrolytic enzymes in brackish, saline and hypersaline lakes of Romania. Microorganisms, 8(12), 1903. DOI

Saxena, A. K., Kumar, M., Chakdar, H., Anuroopa, N., & Bagyaraj, D. J. (2020). Bacillus species in soil as a natural resource for plant health and nutrition. Journal of Applied Microbiology, 128(6), 1583–1594. DOI

Schaad, N. W., Jones, J., & Chun, W. (2001). Laboratory guide for identification of plant pathogenic bacteria. St Paul, MN: American Phytopathological Society. Retrieved from website

Sedki, R., & El-Mohamedy, R. (2012). Biological control of Pythium root rot of broccoli plants under greenhouse conditions. Journal of Agricultural Technology, 8(3), 1017–1028. Retrieved from website

Shilev, S. (2020). Plant-growth-promoting bacteria mitigating soil salinity stress in plants. Applied Sciences, 10(20), 7326; DOI

Shrivastava, P., & Kumar, R. (2015). Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi Journal of Biological Sciences, 22(2), 123–131. DOI

Shtereva, L. A., Vassilevska-Ivanova, R. D., & Karceva, T. V. (2015). Effect of salt stress on some sweet corn (Zea mays L. var. saccharata) genotypes. Archives of Biological Sciences, 67(3), 993–1000. DOI

Teo, H. M., Aziz, A., Wahizatul, A. A., Bhubalan, K., Siti, N. M. S., Muhamad, S. C. I., & Ng, L. C. (2022). Setting a plausible route for saline soil-based crop cultivations by application of beneficial halophyte-associated bacteria: A review. Microorganisms, 10(3), 657. DOI

Yañez-Yazlle, M. F., Romano-Armada, N., Acreche, M. M., Rajal, V. B., & Irazusta, V. P. (2021). Halotolerant bacteria isolated from extreme environments induce seed germination and growth of chia (Salvia hispanica L.) and quinoa (Chenopodium quinoa Willd.) under saline stress. Ecotoxicology and Environmental Safety, 218, 112273. DOI

Zafar-ul-Hye, M., Farooq, H. M., Zahir, Z. A., Hussain, M., & Hussain, A. (2014). Application of ACC-deaminase containing rhizobacteria with fertilizer improves maize production under drought and salinity stress. International Journal of Agriculture and Biology, 16(3), 591–596. Retrieved from PDF

Zhou, N., Zhao, S., & Tian, C.-Y. (2017). Effect of halotolerant rhizobacteria isolated from halophytes on the growth of sugar beet (Beta vulgaris L.) under salt stress. FEMS Microbiology Letters, 364(11), fnx091. DOI




DOI: http://doi.org/10.17503/agrivita.v44i2.3756

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