CHARACTERIZATION AND CLUSTERING OF SOME GUAVA GERMPLASM COLLECTIONS BASED ON LEAF AND FRUIT CHARACTERS

Fitriana Nasution, Sri Hadiati

Abstract


Guava has several different accessions. Guava diversity needs to be studied and evaluated in order to determine the next steps in the guava breeding. The objective of this research was to characterize and cluster of some guava germplasm collections. The study was conducted at Aripan and Subang experimental farm, Indonesian Tropical Fruit Research Institute from January 2012 to December 2012. Five fruits of each accession were randomly selected sampled and then characterized using UPOV guidelines. Obtained data were analyzed by NTSYS ver.2.1. The Similarity level of 19 accessions ranged from 70 – 90% or the genetic distance was between 0-20%. Dendogram obtained could be clustered into two different groups, namely group I (ARP9406, ARP9407, ARP8653, ARP8742, ARP10.2, JBT001, JBT002, ARP8740, JBT003 and JBT004), group II (ARP10.7, ARP10.6, ARP10.1, ARP10.12, ARP10.9, ARP10.11, ARP8744, ARP8741 and ARP8743). The result of this research can be used for guava breeding. Species diversity and genetic resources are very important to get new varieties. This study would be highly valuable in the future.

 

Keywords :  Guava  Germplasm, Characterization, Clustering


Full Text:

PDF

References


Adnyana, I. K., E. Yulinah, J. I. Sigit, N. Fisheri and M. Insanu. 2004. The effect of white guava and redguava leaves extracts as antidiarrheal. Acta Pharmaceutica Indonesia. XXIX (1): 19-27.

Astawan, I.W.S. 2013. The effect of guava (Psidium guajava L.) to dyslipidemia patients. Calyptra: The science journal of Surabaya university students. 2(1): 1-10

Beer, S.C., J. Goffreda, T.D. Phillips, J.P. Murphy and M.E. Sorrells. 1993. Assesment of Genetic Variation in Avenasterilis using Morphological Traits, Isozymes, and RFLPs. Crop Sci. 33: 1386 – 1393.

Cahyarini, R.D., A. Yunus dan E. Purwanto. 2004. Identification of genetic diversity of some soybean varieties in Java by isozyme analysis. Agrosains. 6(2):79 – 83.

Chan Y. K., P. Raveendranathan, M.L. Raziah and S.T. Choo. 1992. Planting Papaya. MARDI Malaysia. Kuala Lumpur, pp. 1-17.

Crossa, J., S. Taba, S.A. Eberhart, P. Bretting and R. Vencovshy. 1995a. Practical Considering for MaintaningGermplasm in Maize. Theor. Appl. Genet. 89: 89 – 95.

Crossa, J., K. Basford, S. Taba, I. DeLacy and E. Silva. 1995b. Three-mode Analyses of Maize using Morphological and Agronomic Attribute Measured in Multilocation Trials. Crop Sci. 35: 1483 – 1491.

Daud, M.F., E.R. Sadiyah and E. Rismawati. 2011. The effect of difference extract method to antioxidant and ethanol extract of white guava (Psidium guajava L.) leaves.Sna PP2011 Sains, Technology, and Healthy Proceeding. 2(1): 55-62.

Infante, J.V., N.N. Rodriguez, D. Becker, B. Velazquez, D. Sourd, G. Espinosa and W. Rohde. 2007. Microsatellite characterization of guava (Psidium guajava L.) germplasm collection in Cuba. CultivosTropicales. 28(3): 61-67.

Joseph, B. 2011. Review on Nutritional, Medicinal and Pharmacological Properties of guava (Psidiumguajava Linn.). International Journal of Pharma and Bio Science. 2(1): 53-69.

Lamadji, S. 1998. The empowerment of morphological character for sugarcane germplasm genetic relationship analysis. P3G1 Bulletin. 148: 17 – 31.

Liu, X. and G. Yang. 2012. Assessment of clonal fidelity of micro-propagated guava (Psidiumguajava) plants by ISSR markers. Australian Journal of Crop Science. 6(2): 291-295.

Mani, A., R. Mishra and G. Thomas. 2011. Elocidation of Diversity among Psidiumspecies using Morphological and SPAR methods. Journal of Phytology, 3 (8): 53-61.

Anonimous. 2011. Taiwan guava production manual.Taiwan Ministry of Agriculture and Fisheries.35p.

Owen, P.L., L. C. Martineau, D. Caves, P. S. Haddad, T. Matainaho and T. Johns. 2008. Consumption of guava (Psidium guajava L) and noni (Morinda citrifolia L) may protect betel quid-chewing Papua New Guineans against diabetes. Asia Pac. J. clin. Nutr. 17(4): 635-643.

Rai, M.K., M.P. Harish, A.K. Gupta, N.S. Shekhawat and U. Jaiswal. 2012. Genetic homogeneity of guava plants derived from somatic embryogenesis using SSR and ISSR markers. Plant Cell Tiss Organ Cult. 111: 259-264.

Sharma, A., S. K. Sehrawat, R. S. Singhrat and A. Tele. 2010. Morphological and Chemical Characterization of Psidium Species. Notulae Botanicae Horti Agrobotarici Cluj. Napoja. 38(1): 28-32

Tatineni, V., G. Cantrell and D.D. Davis. 1996. Genetic diversity in elite cotton germplasm determined by morphological characteristics and RAPDs. Crop Sci. 36:186 - 192.

UPOV. 1987. Guidelines for the conduct of tests for distinctness, Homogeneity and Stability: Guava (Psidium guajava). Geneva (Switzerland). 27p.




DOI: http://doi.org/10.17503/agrivita.v36i1.370