Exploring The Potency of Microalgae-Based Biofertilizer and Its Impact on Oil Palm Seedlings Growth

Indiani Sani, Yudistira Wahyu Kurnia, Hana Christine Sinthya, Richard Anthony, Elizabeth Caroline Situmorang, Condro Utomo, Tony Liwang


Indonesia is currently the world’s largest palm oil producer. Consequently, the use of chemical fertilizers become more extensive. There is a need to explore sustainable alternative sources of plant nutrition.  Microalgae represents a potential sustainable alternative for the enhancement and protection of crops based on their cell elements. In this study, dry biomass or liquid culture formulation of the green microalgae Haematococcus pluvialis was applied to the rhizosphere of oil palm pre-nursery as a biofertilizer.  Soil application of microalgae biomass of 0.5 g/L (MA) or liquid culture of 10% (v/v) (BCMA) was carried out to assess its effects on 4 months old oil palm at the nursery stage. The compatibility test between microalgae and common biofungicide agent in agricultural practices, Trichoderma spp., was also tested on both microalgae formulations. The result showed that both microalgae biomass and liquid culture, alone or combined with Trichoderma spp., gave a better growth performance to the oil palm. MA and BCMA application had resulted in a maximum increment of plant height, leaves count, and chlorophyll content. Furthermore, application of the BCMA gave the better oil palm growth performance, which may probably influenced by the accessibility of nutrient for microalgae’s growth., the study revealed that the application of microalgae as biofertilizer has potential to improve oil palm growth performance as an alternative to replace the chemical fertilizer.


Haematococcus pluvialis, Microalgae, Pre nursery, Vegetative performance

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Abdel-Raouf N. (2012). Agricultural importance of algae. African Journal of Biotechnology, 11(54), 11648–11658. https://doi.org/10.5897/ajb11.3983

Abinandan, S., Subashchandrabose, S. R., Venkateswarlu, K., & Megharaj, M. (2019). Soil microalgae and cyanobacteria: the biotechnological potential in the maintenance of soil fertility and health. Critical Reviews in Biotechnology, 39(8), 981–998. https://doi.org/10.1080/07388551.2019.1654972

Agwa, O. K., Ogugbue, C. J., & Williams, E. E. (2017). Field Evidence of Chlorella vulgaris Potentials as a Biofertilizer for Hibiscus esculentus. International Journal of Agricultural Research, 12(4), 181–189. https://doi.org/10.3923/ijar.2017.181.189

Ajeng, A. A., Abdullah, R., Malek, M. A., Chew, K. W., Ho, Y. C., Ling, T. C., Lau, B. F., & Show, P. L. (2020). The effects of biofertilizers on growth, soil fertility, and nutrients uptake of oil palm (Elaeis guineensis) under greenhouse conditions. Processes, 8(12), 1–16. https://doi.org/10.3390/pr8121681

Alvarez, A. L., Weyers, S. L., Goemann, H. M., Peyton, B. M., & Gardner, R. D. (2021). Microalgae, soil and plants: A critical review of microalgae as renewable resources for agriculture. Algal Research, 54(February), 102200. https://doi.org/10.1016/j.algal.2021.102200

Ayanda, A. F., Jusop, S., Ishak, C. F., & Othman, R. (2020). Utilization of magnesium-rich synthetic gypsum as magnesium fertilizer for oil palm grown on acidic soil. PLoS ONE, 15(6 June), 1–17. https://doi.org/10.1371/journal.pone.0234045

Casella, P., Iovine, A., Mehariya, S., Marino, T., Musmarra, D., & Molino, A. (2020). Smart method for carotenoids characterization in haematococcus pluvialis red phase and evaluation of astaxanthin thermal stability. Antioxidants, 9(5), 1–17. https://doi.org/10.3390/antiox9050422

Chen, D., Hou, Q., Jia, L., & Sun, K. (2021). Combined Use of Two Trichoderma Strains to Promote Growth of Pakchoi (Brassica chinensis L.). Agronomy, 11(4), 726. https://doi.org/10.3390/agronomy11040726

Contreras-Cornejo, H. A., Ortiz-Castro, R., López-Bucio, J., & Mukherjee, P. K. (2013). Promotion of plant growth and the induction of systemic defence by Trichoderma: physiology, genetics and gene expression. Trichoderma: Biology and Applications, 175, 96.

Coppens, J., Grunert, O., Van Den Hende, S., Vanhoutte, I., Boon, N., Haesaert, G., & De Gelder, L. (2016). The use of microalgae as a high-value organic slow-release fertilizer results in tomatoes with increased carotenoid and sugar levels. Journal of Applied Phycology, 28(4), 2367–2377. https://doi.org/10.1007/s10811-015-0775-2

Costa, O. Y. A., Raaijmakers, J. M., & Kuramae, E. E. (2018). Microbial extracellular polymeric substances: Ecological function and impact on soil aggregation. Frontiers in Microbiology, 9(JUL), 1–14. https://doi.org/10.3389/fmicb.2018.01636

CPOPC. (2020). Palm oil suply demand outlook report 2020 (p. 10). CPOPC. Retrieved from https://www.cpopc.org/wp-content/uploads/2020/04/Palm-Oil-Supply-Demand-Outlook-2020-EDIT-Final.pdf

Dash, N. P., Kumar, A., Kaushik, M. S., & Singh, P. K. (2016). Cyanobacterial (unicellular and heterocystous) biofertilization to wetland rice influenced by nitrogenous agrochemical. Journal of Applied Phycology, 28(6), 3343–3351. https://doi.org/10.1007/s10811-016-0871-y

Dineshkumar, R., Ahamed Rasheeq, A., Arumugam, A., Nathiga Nambi, K. S., & Sampathkumar, P. (2019). Marine microalgal extracts on cultivable crops as a considerable bio-fertilizer: A review. Indian Journal of Traditional Knowledge, 18(4), 849–854.

El-Baz, F. K., Hussein, R. A., Mahmoud, K., & Abdo, S. M. (2018). Cytotoxic activity of carotenoid rich fractions from Haematococcus pluvialis and Dunaliella salina microalgae and the identification of the phytoconstituents using LC-DAD/ESI-MS. Phytotherapy Research, 32(2), 298–304. https://doi.org/10.1002/ptr.5976

Elarroussi, H., Elmernissi, N., Benhima, R., Meftah El Kadmiri, I., Bendaou, N., & Smouni, A. (2016). Microalgae polysaccharides a promising plant growth biostimulant. J. Algal Biomass Utln, 7(4), 55–63.

Elshahawy, I. E., & El-Sayed, A. E. K. B. (2018). Maximizing the efficacy of trichoderma to control cephalosporium maydis, causing maize late wilt disease, using freshwater microalgae extracts. Egyptian Journal of Biological Pest Control, 28(1), 1–11. https://doi.org/10.1186/s41938-018-0052-1

Garcia-Gonzalez, J., & Sommerfeld, M. (2016). Biofertilizer and biostimulant properties of the microalga Acutodesmus dimorphus. Journal of Applied Phycology, 28(2), 1051–1061. https://doi.org/10.1007/s10811-015-0625-2

Gonçalves, A. L. (2021). The use of microalgae and cyanobacteria in the improvement of agricultural practices: A review on their biofertilising, biostimulating and biopesticide roles. Applied Sciences (Switzerland), 11(2), 1–21. https://doi.org/10.3390/app11020871

González, A., Castro, J., Vera, J., & Moenne, A. (2013). Seaweed Oligosaccharides Stimulate Plant Growth by Enhancing Carbon and Nitrogen Assimilation, Basal Metabolism, and Cell Division. Journal of Plant Growth Regulation, 32(2), 443–448. https://doi.org/10.1007/s00344-012-9309-1

Guedes, A. C., Amaro, H. M., & Malcata, F. X. (2011). Microalgae as sources of carotenoids. Marine Drugs, 9(4), 625–644. https://doi.org/10.3390/md9040625

Halifu, S., Deng, X., Song, X., & Song, R. (2019). Effects of two Trichoderma strains on plant growth, rhizosphere soil nutrients, and fungal community of Pinus sylvestris var. mongolica annual seedlings. Forests, 10(9), 1–17. https://doi.org/10.3390/f10090758

Jochum, M., Moncayo, L. P., & Jo, Y. K. (2018). Microalgal cultivation for biofertilization in rice plants using a vertical semi-closed airlift photobioreactor. PLoS ONE, 13(9), 1–13. https://doi.org/10.1371/journal.pone.0203456

Kaushik, B. D. (2014). Developments in cyanobacterial biofertilizer. Proceedings of the Indian National Science Academy, 80(2), 379–388. https://doi.org/10.16943/ptinsa/2014/v80i2/55115

Kholssi, R., Marks, E. A. N., Miñón, J., Montero, O., Debdoubi, A., & Rad, C. (2019). Biofertilizing Effect of Chlorella sorokiniana Suspensions on Wheat Growth. Journal of Plant Growth Regulation, 38(2), 644–649. https://doi.org/10.1007/s00344-018-9879-7

Kumar, A., & Singh, J. S. (2020). Microalgal bio-fertilizers. In Handbook of Microalgae-Based Processes and Products. Elsevier Inc. https://doi.org/10.1016/b978-0-12-818536-0.00017-8

Lichtenthaler, H. K., & Buschmann, C. (2001). Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy. Current Protocols in Food Analytical Chemistry, 1(1), F4.3.1-F4.3.8. https://doi.org/10.1002/0471142913.faf0403s01

Liu, X., Zhang, M., Liu, H., Zhou, A., Cao, Y., & Liu, X. (2018). Preliminary characterization of the structure and immunostimulatory and anti-aging properties of the polysaccharide fraction of: Haematococcus pluvialis. RSC Advances, 8(17), 9243–9252. https://doi.org/10.1039/c7ra11153c

López-Arredondo, D. L., Leyva-González, M. A., Alatorre-Cobos, F., & Herrera-Estrella, L. (2013). Biotechnology of nutrient uptake and assimilation in plants. International Journal of Developmental Biology, 57(6–8), 595–610. https://doi.org/10.1387/ijdb.130268lh

Lu, Y., & Xu, J. (2015). Phytohormones in microalgae: A new opportunity for microalgal biotechnology? Trends in Plant Science, 20(5), 273–282. https://doi.org/10.1016/j.tplants.2015.01.006

Mahanty, T., Bhattacharjee, S., Goswami, M., Bhattacharyya, P., Das, B., Ghosh, A., & Tribedi, P. (2017). Biofertilizers: a potential approach for sustainable agriculture development. Environmental Science and Pollution Research, 24(4), 3315–3335. https://doi.org/10.1007/s11356-016-8104-0

Maqubela, M. P., Mnkeni, P. N. S., Muchaonyerwa, P., D’acqui, L. P., & Pardo, M. T. (2010). Effects of cyanobacteria strains selected for their bioconditioning and biofertilization potential on maize dry matter and soil nitrogen status in a South African soil. Soil Science and Plant Nutrition, 56(4), 552–559. https://doi.org/10.1111/j.1747-0765.2010.00487.x

Morais. (2013). Influence of the Growth Regulators Kinetin and 2,4-D on the Growth of Two Chlorophyte Microalgae, Haematococcus pluvialis and Dunaliella salina. Journal of Basic & Applied Sciences, March 2018. https://doi.org/10.6000/1927-5129.2013.09.40

Ortiz-Moreno, M. L., Sandoval-Parra, K. X., & Solarte-Murillo, L. V. (2019). Chlorella, a potential biofertilizer? Orinoquia, 23(2), 71–78. https://doi.org/10.22579/20112629.582

Paudel, Y., & Pradhan, S. (2012). Role of blue green algae in rice productivity. Agriculture and Biology Journal of North America, 3(8), 332–335. https://doi.org/10.5251/abjna.2012.3.8.332.335

Pereira, I., Ortega, R., Barrientos, L., Moya, M., Reyes, G., & Kramm, V. (2009). Development of a biofertilizer based on filamentous nitrogen-fixing cyanobacteria for rice crops in Chile. Journal of Applied Phycology, 21(1), 135–144. https://doi.org/10.1007/s10811-008-9342-4

Rivera-Solís, R. A., Peraza-Echeverria, S., Echevarría-Machado, I., & Herrera-Valencia, V. A. (2014). Chlamydomonas reinhardtii has a small family of purple acid phosphatase homologue genes that are differentially expressed in response to phytate. Annals of Microbiology, 64(2), 551–559. https://doi.org/10.1007/s13213-013-0688-8

Saadaoui, I., Al Ghazal, G., Bounnit, T., Al Khulaifi, F., Al Jabri, H., & Potts, M. (2016). Evidence of thermo and halotolerant Nannochloris isolate suitable for biodiesel production in Qatar Culture Collection of Cyanobacteria and Microalgae. Algal Research, 14, 39–47. https://doi.org/10.1016/j.algal.2015.12.019

Saadaoui, I., Sedky, R., Rasheed, R., Bounnit, T., Almahmoud, A., Elshekh, A., Dalgamouni, T., al Jmal, K., Das, P., & Al Jabri, H. (2019). Assessment of the algae-based biofertilizer influence on date palm (Phoenix dactylifera L.) cultivation. Journal of Applied Phycology, 31(1), 457–463. https://doi.org/10.1007/s10811-018-1539-6

Serwotka-Suszczak, A. M., Marcinkowska, K. A., Smieszek, A., Michalak, I. M., Grzebyk, M., Wiśniewski, M., & Marycz, K. M. (2019). The Haematococcus pluvialis extract enriched by bioaccumulation process with Mg(II) ions improves insulin resistance in equine adipose-derived stromal cells (EqASCs). Biomedicine and Pharmacotherapy, 116(March). https://doi.org/10.1016/j.biopha.2019.108972

Shireen, F., Nawaz, M. A., Chen, C., Zhang, Q., Zheng, Z., Sohail, H., Sun, J., Cao, H., Huang, Y., & Bie, Z. (2018). Boron: Functions and approaches to enhance its availability in plants for sustainable agriculture. International Journal of Molecular Sciences, 19(7), 95–98. https://doi.org/10.3390/ijms19071856

Situmorang, E. C., Prameswara, A., Sinthya, H. C., Toruan-Mathius, N., & Liwang, T. (2013). Morphology and Histology Identification of Fungal Endophytes from Oil Palm Roots in Ganoderma boninense Endemic Area. Microbiology Indonesia, 7(4), 9.

Toribio, A. J., Suárez-Estrella, F., Jurado, M. M., López, M. J., López-González, J. A., & Moreno, J. (2020). Prospection of cyanobacteria producing bioactive substances and their application as potential phytostimulating agents. Biotechnology Reports, 26. https://doi.org/10.1016/j.btre.2020.e00449

Tripathi, R. D., Dwivedi, S., Shukla, M. K., Mishra, S., Srivastava, S., Singh, R., Rai, U. N., & Gupta, D. K. (2008). Role of blue green algae biofertilizer in ameliorating the nitrogen demand and fly-ash stress to the growth and yield of rice (Oryza sativa L.) plants. Chemosphere, 70(10), 1919–1929. https://doi.org/10.1016/j.chemosphere.2007.07.038

Uysal, O., Uysal, F. O., & Ekinci, K. (2015). Evaluation of microalgae as microbial fertilizer. European Journal of Sustainable Development, 4(2), 77.

Zhao, L., Wang, Y., & Kong, S. (2020). Effects of Trichoderma asperellum and its siderophores on endogenous auxin in Arabidopsis thaliana under iron-deficiency stress. International Microbiology, 23(4), 501–509. https://doi.org/10.1007/s10123-020-00122-4

DOI: http://doi.org/10.17503/agrivita.v44i1.3102

Copyright (c) 2021 Indiani Sani, Yudistira Wahyu Kurnia, Hana Christine Sinthya, Richard Anthony, Elizabeth Caroline Situmorang, Condro Utomo, Tony Liwang

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