Genetic Diversity of Perennial Wild Species of Alfalfa Subgenus Falcago (Reichb) Grossh. in Kazakhstan and Their Involvement in the Breeding

Bauyrzhan Bakytzhanovich Kalibayev, Galiolla Tulendinovich Meiirman, Sakysh Tanyrbergenovna Yerzhanova, Serik Sarybaevich Abaev, Amankeldi Turgambekovich Kenebaev


The field expedition has collected 144 samples at the ecotype level of seven wild alfalfa species, which are the source of adaptive properties. One part of the original seeds has been placed for medium-term storage as the gene pool, and the other part has been sown in the culture to use wild-growing species in recurrent breeding. It has been found that the productivity of wild species is inferior to that of M. sativa L., but they are valuable as a source of adaptation traits: drought tolerance, salt tolerance, and winter hardiness, which are important with the development of recurrent breeding to adapt the crops to the global climate change. The yield of the hybrid plants in the offspring from crossing seven ecotypes of M. falcata L. (yellow-flowered) with M. sativa L. (blue-flowered) using a marker-trait has been established: the hybrid nature of the plants obtained from free entomophilic cross-pollination has been established, and the yield of the hybrid plants has amounted to 61 – 91 %. In the hybrid population obtained from M. sativa sybsp. transoxona, M. falcata L., and M. tianschanica Vass. upon crossing them, 50 best phenotypes have been selected to continue the backcrossing with M. sativa L.


adaptation traits, gene pool, hybrid population, recurrent breeding, tetraploid and diploid alfalfa species


Chernyavskikh, V. I., Dumacheva, E. V., & Borodaeva, Z. A. (2019). Osobennosti adaptatsii ekotipov medicago varia m. k razlichnym usloviyam ekotop [Peculiarities of the medicago varia m. ecotypes adaptation to various conditions of the ecotopes]. Proceedings Of The V International Scientific And Methodological Conference"Role Of Physiology And Biochemistry In Introduction And Breeding Of Agricultural Plants". In 2 Volumes, 104–107.

Dzyubenko, N. I., Bukhteeva, A. V., & Kochegina, A. A. (2017). Perennial And Annual Drought-and Salt-Resistant Forage Plants in the Vavilov Collection. Proceedings on Applied Botany, Genetics And Breeding, 178(1), 5–23.

Humphries, A. W., Ovalle, C., Hughes, S., del Pozo, A., Inostroza, L., Barahona, V., Yu, L., Yerzhanova, S., Rowe, T., Hill, J., Meiirman, G., Abayev, S., Brummer, E. C., Peck, D. M., Toktarbekova, S., Kalibayev, B., Espinoza, S., Ivelic‐Saez, J., Bingham, E., … Kilian, B. (2020). Characterization, preliminary evaluation and pre‐breeding of diverse alfalfa crop wild relatives originating from drought‐stressed environments. Crop Science, csc2.20274.

Kenzhebaevich, N. S., Tolendinovich, M. G., Saribaevich, A. S., Mansurovna, B. K., & Saylauvna, Y. R. (2018). Selection of Inbred Lines of Alfalfa for Creating Synthetic Varieties. OnLine Journal of Biological Sciences, 18(1), 7–16.

Krasteva, L., Uzundzhalieva, K., & Ruseva, R. (2012). Plant Genetic Resources as a part of the biodiversity. Агрознање, 13(1).

Kumar, T., Bao, A.-K., Bao, Z., Wang, F., Gao, L., & Wang, S.-M. (2018). The progress of genetic improvement in alfalfa (Medicago sativa L.). Czech Journal of Genetics and Plant Breeding, 54(No. 2), 41–51.

Lala, S., Amri, A., & Maxted, N. (2018). Towards the conservation of crop wild relative diversity in North Africa: checklist, prioritisation and inventory. Genetic Resources and Crop Evolution.

Lapina, L., Grauda, D., Rashal, I., Lapiņa, L., Grauda, D., & Rashal, I. (2011). Characterization of latvian alfalfa Medicago sativa genetic resources. Acta Biol. Univ. Daugavp., 11(2).

Liatukiene, A., Skuodiene, R., Tomchuk, D., & Danyte, V. (2020). Evaluation of agro-biological traits of Medicago sativa and M. varia in a Cambisol and Retisol. Zemdirbyste-Agriculture, 107(1), 41–48.

Meirman, G., Kenenbayev, S., Yerzhanova, S., Abayev, S., & Toktarbekova, S. (2017). Results of Selection Studies of Alfalfa Based on Inbred Lines. Journal of Agricultural Science and Technology A, 7(5), 309–316.

Meiirman, G. T., Erzhanova, S. T., Abayev, S. S., Seitkarimov, A., & Al., E. (2017). Netraditsionnye i dikorastushchie kormovye rasteniya i ikh introduktsii [Non-traditional and wild-growing forage plants and their introductions].

Meiirman, G. T., & Masonichich-Shotunova, R. (2012). Lyutserna [Alfalfa].

Meiirman, G. T., & Yerzhanova, S. T. (2017). The formation and study in the culture of Genetic Resources of forage crops by the exspeditionary collection of wild forms from the natural landscapes of Kazakhstan. Ekin Journal “Crop Breeding and Genetics,” 1(2), 70–77.

Meiirman, G. T., Yerzhanova, S. T., Abayev, S. S., & Toktarbekova, S.T., Kenebaev, A. T. (2016). Creation of highly productive polycomponent agrocoenosis of fodder crops to improve the quality of fodder. Science and World, 5(33), 69–75.

Prosperi, J.-M., Jenczewski, E., Muller, M.-H., Fourtier, S., Sampoux, J.-P., & Ronfort, J. (2014). Alfalfa domestication history, genetic diversity and genetic resources. Legume Perspectives, 4, 13–14.

Shi, S., Nan, L., & Smith, K. (2017). The Current Status, Problems, and Prospects of Alfalfa (Medicago sativa L.) Breeding in China. Agronomy, 7(1), 1.

Toktarbekova, S., Meiirman, G., Yerzhanova, S., Abayev, S., & Umbetov, A. (2020). Productivity of the Green Mass of New Alfalfa Cultivars Depending on the Effect of Macro- and Microfertilizers on Various Phosphorous Backgrounds. Journal of Ecological Engineering, 21(2), 57–62.

Xu, Z., Zhou, G., & Shimizu, H. (2010). Plant responses to drought and rewatering. Plant Signaling & Behavior, 5(6), 649–654.

Yerzhanova, S., S., A., & Erzhebaeva, R. (2015). The result of alfalfa breeding for intensive fodder production in Kazakhstan. The 5th Intrnational Symposium of Forage Breeding, 610–612.

Zubair, H. M., Pratley, J. E., Sandral, G. A., & Humphries, A. (2017). Allelopathic interference of alfalfa (Medicago sativa L.) genotypes to annual ryegrass (Lolium rigidum). Journal of Plant Research, 130(4), 647–658.


Copyright (c) 2021 Bauyrzhan Bakytzhanovich Kalibayev, Galiolla Tulendinovich Meiirman, Sakysh Tanyrbergenovna Yerzhanova, Serik Sarybaevich Abaev, Amankeldi Turgambekovich Kenebaev

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.