ENHANCEMENT IN EFFECTIVENESS OF ANTAGONISTIC MICROBE BY MEANS OF MICROBIAL COMBINATION TO CONTROL Ralstonia solanacearum ON POTATO PLANTED IN MIDDLE LATITUDE

Anis Rosyidah, Tatik Wardiyati, Abdul Latief Abadi, Moch. Dawam Maghfoer

Abstract


One of the common problems hampering the cultivation of potatoes in middle latitude is the presence of bacterium Ralstonia solanacearum commonly known to cause wilt disease, by which crop failure might be caused when serious attack occurs. The objectives of the research were to obtain the application of antagonistic microbes to inhibit the wilt disease caused by R.solanacearum and to increase the growth and yield of potato in middle latitude. The research was conducted from July to October 2012. Antagonistic microbes were used in this research to inhibit the bacterial wilt disease caused by R.solanacearum on potato planted in Bumiaji, Batu. Trichoderma viride, Streptomyces sp. and Pseudomonas fluorescens isolates were selected to be applied as treatments either singly or in combination. Randomised block design was applied on the treatment group with three replications. The results showed that the single application of Pseudomonas fluorescens or combined application of Streptomyces sp. and Trichodermaviride + Streptomycessp. was capable of extending incubation period 4 to 7 days and reducing disease incidence  44.85% - 50.09%, reducing disease intensity up to 61.23 - 72.77%, reducing the population R.solanacearum up to 7.28 - 97.88%, increasing the number of leaves and the marketable yield as much as 67.96 - 81.98%.

Keywords: control, Ralstonia solanacearum, antagonistic microbes, middle latitude


Full Text:

PDF

References


Arwiyanto, T. 1998. Biological Control on Wilt Disease due to Bacteria in Tobacco. ( in Indonesia) Integrated Excellent Research Report IV (1996-1998). pp.58

Baharuddin, Nursaba and T. Kuswinanti. 2005. Impacts of giving Pseudomonas fluorescens and Effective Organism in Hampering Wilt Disease Bacterium (Ralstonia solanacearum) in Chilli (Capsi-cum annum L.). ( in Indonesia) Scientific Seminar and Annual Meeting of PEI and PFI XVI Komda South Sulawesi, 2005.ISBN : 979-95025-6-7: 195-200.

Bisnis Indonesia Online. 2008. Potato Seed gets more Costly. (in Indonesia) http://www. w3.org/TR/html (accessed on April 23, 2009)

Campbell, R. 1989. Biological control of microbial plant pathogens.Cambridge University Press. Cambridge. p. 218

Djatmiko, H.A., T. Arwiyanto, T. Hadisutrisno and B.H Sunarminto. 2007. Potential of three Genera of Bacteriafrom Three Crop Rhizospheres as A Biological Control Agent for Lincat Disease.(in Indonesia). J. lmu-Ilmu pertanian Indonesia.vol 9: 40-47

Duffy, B.K. and G. Defago. 1999. Environmental factor modulating antibiotic and siderophore biosynthesis by Pseudomo-nas fluorescens Strains. App. Env. Microb. 65(6): 2429-2438

Duijff, B.J., J.W. Meijer, P.A.H.M. Bakker and B. Schippers. 1993. Siderophore-mediated competition for iron and induced resistence in the suppression of Fusarium wilt of carnation by Pseudomonas fluorescens. Netherland Journal of Plant Pathology 99: 277-289

Ernawati, N.M. 2003. Potential of antagonistic micro-organism of soil in hampering Pseudomonas solanacearumin in vitro banana in Lombok Island.(in Indonesia) http://tumoutou.net/70207134/nml.ernawati.htm (accessed on July 12, 2008)

Glick, R.B. 1995. The enhancement of plant growth promotion by free living bacteria. J. Microbiol 41:109-117

Guetsky, R., D. Shtienberg, Y. Elad, and A. Dinoor. 2001. Combining biocontrol agents to reduce the variability of biological control. Phytopathology 91(43): 621-627

Gunawan, O.S., 1995. Effects of antagonistic microbes in controlling wilt disease bacterium Pseudomonas solanacearum in potatoes.(in Indonesia). Treatise of National Congress XIII and Scientific Seminar of PFI. Mataram, September 25-27, 1995. p. 473-479.

Hanudin, B. Marwoto, Hersanti and A. Muharam. 2012. Compatibility of Bacillus subtilis, Pseudomonas fluorescens and Tricho-derma harzianum in controlling Ralstonia solanacearum in potatoes. ( in Indonesia) J.Hortikultura. 22(2): 173-180

Hasanah, F., A. Rosyidah and I. Murwani. 2013. Application of Pseudomonas fluorescens and several organic matters in controlling Ralstonia solanacearum in Potatoes (Solanum tuberosum L.). ( in Indonesia). J. Agronisma. 1(1):1-12

Hersanti, R. T., A. Rupendi, Purnama, Hanudin, B. Marwoto and O. S. Gunawan. 2009. Filtration of some isolates Pseudomonas fluorescens, Bacillus subtilis and Trichoderma harzianum Antagonistic to R. solanacearum in potatoes. ( in Indonesia) J.Agrikultura 20(3): 198-203

Heru. 2006. Study of biological control for lincat disease in tobacco by combining Pseudomonas fluorescens, Bacillus spp., and Streptomyces spp. ( in Indonesia) Dissertation. Postgraduate Program. Gajah Mada University. Yogyakarta (unpublished)

Khairul, U. 2006. Study of Streptomyces sp. utilization as biological control agents for bacterial wilt Ralstonia solanacearum on chili. (in Indonesia). http://repository. unand.ac.id/2005/

Landa, B.B., H.A.E de Werd, B.B. Mcspadden Gardener and D.M. Weller. 2002. Comparison of three methods for monitoring populations of different genotypes of 2,4-diacethylphloroglucinol-producing Pseudomonas fluorescens in rhizosphere. Phytopathology 92: 129-137

Nasrun, Christanti, T. Arwiyantoand I. Mariska. 2005. controlling wilt disease caused by bacteria in patchouli using Pseudomonas fluorescens. (in Indonesia) J. Penelitian Tanaman Industri. 11(1): 19-24

Nurbaya., M, D. Rahim, T. Kuswinanti and Baharuddin.2011. Combined effect of isolate antagonistic bacteria to bacterial wilt disease (Ralstonia solanacearum) raising the potato crop aeroponics (in Indonesia). http://www.peipfi-komdasulsel. org. (accessed on July 19, 2011)

Nurjajani. 2011. Study of biological control agents in wilt disease bacterium (Ralstonia solanacearum). (in Indonesia). Suara Perlindungan Tanaman.1(4):1-8

Park, K.H., C.Y. Lee, and H.J. Son. 2009. Mechanism of insoluble phosphate solubilization by Pseudomonas fluores-cens RAF15 isolated from ginseng rhizos-phere and its plant growth promoting activities. Letters in Applied Microbiology 49: 222–228.

Rosyidah, A. 2010. Effects of organic matters and application time of biological agent Trichoderma sp. on potato yields in Middle Latittudes.(in Indonesia).Thesis. Postgra-duate Program of Brawijaya University. Malang. pp 76. Uplibished.

Samanhudi. 2009. Screening on endurance of potato clone to bacterial wilt disease. ( in Indonesia). Agriculture Faculty of Sebelas Maret University. http://pertanian.uns. ac.id (accessed on June 16, 2009)

Sastra, D.R. 2009. Incubation period of pathogenic bacterium Ralstonia solanacearum RAS 3 in several potato clones. (in Indonesia) J. Agronomi. 8(1):63-67

Shimizu. 2002. Studies on endophyti cactino-mycetes (I) Streptomyces sp. isolated from rhododendron and its antifungal activity. General Plant Pathology. 66:3 60-366

Sinaga, M.S.2006. Basics of plant pathology .(in Indonesia). Penebar Swadaya. Jakarta. pp.136

Soesanto, L. 2004. Ability of P. fluorescens P60 as a biological control agent for rot disease on stems of in vivo peanuts.( in Indonesia) Eugenia. 10(1):8-17

Soesanto, L., Rokhlani and N. Prihatiningsih. 2008. Hampering microorganims anta-gonistic to fusarium wilt disease in gladioli. (in Indonesia). J. Agrivita. 30(1): 75-83

Soesanto, L., E. Mugiastuti and R.F. Rahayuniati. 2011. Inventory and identification of soil-borne pathogens in potato planting in Purbalingga Regency.(in Indonesia) J. Hort. 21(3): 254-264

Semangun, H. 2002. Horticultural diseases in Indonesia. (in Indonesia) Gajah Mada Press.Yogyakarta. pp.850.

Thakuria D, Talukdar NC, Goswani C, Hazarika S, Boro RC. 2004. Characterization and screening of bacteria from rhizosphere of rice grown in acidic soils of assam. Current Science 83: 1140-1143.

Tuju, M.J. 2004. Test of antagonisme Trichoderma spp. to R. Solanacearum cause wilt bacteria disease in potato plant. Eugenia 10(2): 143-155

Vasudevan, P. M.S. Reddy, S. Kavitha, P. Velusamy and R.S.D. Paulraj. 2002. Role of biological preparations in enhancement of rice seedling growth and grain yield. Current Science 83: 1140-1143.

Vonderwell, J.D., S.A. Enebak and L.J. Samuelson. 2001. Influence of two plant gowth-promoting rhizobacteria on loblolly pine root respiration and IAA activity. Forest science. 47:197-202.

Wardiyati, T. 1990. Effect of high temperature on the formation of potato (Solanum tuberosum L.) tubers in the lowlands. Disertation. Postgraduate Program Bogor Agricultural University. pp 108

Weller, D.M. 1988. Biological control of soil-borne plant pathogens in the rhizospher with bacteria. Ann. Rev. Phytopathol 26:379-407

Winstead, N. N. and Kelman. 1952. Inoculation techniques for evaluation of re­ sistance to P. solanacearum. Phytopathology 42: 628-634.




DOI: http://doi.org/10.17503/agrivita.v35i2.285