Using Trichoderma species in combination with cattle dung as soil amendment improves yield and reduces pre-harvest aflatoxin contamination in groundnut

Victor Ohileobo Dania


The efficacy of combining five Trichoderma species and cattle dung in the integrated management of aflatoxin-producing Aspergillus flavus under laboratory and field conditions was evaluated. Trichoderma asperellum, T. hamatum, T. viride, T. harzianum and T. pseudokoningii were bioassayed against A. flavus in vitro, while they were applied in combination with cattle dung in the field experiment.  Trichoderma containing 2.3 x 108 cfu/g-1 was formulated using talc powder and applied as seed treatment, while soil application was done using sorghum grains as carrier substrate at 2.5 kg/ha1 and 5t/ha cattle dung. Aflatoxin concentration was quantified using high-performance thin-layer chromatography. The field trial was a randomized complete block design with three replications. Trichoderma harzianum was the most effective with 72.3% mycelia inhibition of A. flavus. Seed treatment with combination of T. harzianum and cattle dung was most effective in reduction of A. flavus soil population to 1.1×103 cfu/g-1 and aflatoxin contamination. The application of T. hamatum and cattle dung in combination produced the highest yield of 2.7 t/ha, which was significantly (p<0.05) higher than the control and carbendazim fungicide. This study has shown the prospect of Tichoderma species and cattle dung in the integrated management of aflatoxin contamination in groundnut.


Aspergillus flavus, Contamination, Efficacy, Thin-layer Chromatography, Yield

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