Using Trichoderma species in combination with cattle dung as soil amendment improves yield and reduces pre-harvest aflatoxin contamination in groundnut

Victor Ohileobo Dania

Abstract


The efficacy of combining five Trichoderma species and cattle dung in the integrated management of aflatoxin-producing Aspergillus flavus under laboratory and field conditions was evaluated. Trichoderma asperellum, T. hamatum, T. viride, T. harzianum and T. pseudokoningii were bioassayed against A. flavus in vitro, while they were applied in combination with cattle dung in the field experiment.  Trichoderma containing 2.3 x 108 cfu/g-1 was formulated using talc powder and applied as seed treatment, while soil application was done using sorghum grains as carrier substrate at 2.5 kg/ha1 and 5t/ha cattle dung. Aflatoxin concentration was quantified using high-performance thin-layer chromatography. The field trial was a randomized complete block design with three replications. Trichoderma harzianum was the most effective with 72.3% mycelia inhibition of A. flavus. Seed treatment with combination of T. harzianum and cattle dung was most effective in reduction of A. flavus soil population to 1.1×103 cfu/g-1 and aflatoxin contamination. The application of T. hamatum and cattle dung in combination produced the highest yield of 2.7 t/ha, which was significantly (p<0.05) higher than the control and carbendazim fungicide. This study has shown the prospect of Tichoderma species and cattle dung in the integrated management of aflatoxin contamination in groundnut.


Keywords


Aspergillus flavus, Contamination, Efficacy, Thin-layer Chromatography, Yield

Full Text:

PDF

References


Amaike, S., & Keller, M. P. (2011). Aspergillus flavus annual review. Phytopathology, 49, 107-133. http://doi: 10.1146/annurev-phyto-072910-095221

Bankole, S. A., Schollenberger, M., & Drochner, W. (2006). Mycotoxins in food systems in sub-Saharan Africa: A review. Mycotoxin Research, 22, 163-169. http://doi: 10.1007/BF02959270

Brady N.C., & Weil, R.R. (2010). Elements of the nature and properties of soils. 3rd ed. Prentice Hall, Upper Saddle River, New Jersey, USA.

Chalwe, H.M., Lungu, O.I., Mweetwa1, A.M., Phiri1, E., Njoroge, S. M. C. Brandenburg, R. L., & Jordan, D.L. (2019). Effects of compost manure on soil microbial respiration, plant-available-water, peanut (Arachis hypogaea L.) yield and pre-harvest aflatoxin contamination. Peanut Science, 46, 42–49. DOI: 10.3146/PS18-6.1

Chiuraise, N., Yobo, K. S., & Laing, M. D. (2015). Seed treatment with Trichoderma harzianum strain kd formulation reduced aflatoxin contamination in groundnuts. Journal of Plant Diseases and Protection, 122(2), 74-80. https://doi.org/10.1007/BF03356534

Cuong N. L., Thai, T.H., Nguyen,X.V., Nguyen, T.L., Tran, T.X.P., &

Tran, T.P.N. (2019) Biological control of groundnut stem rot by Bacillus sp. strain S20D12, Archives of Phytopathology and Plant Protection, 52 (7&8), 625-638. https://doi 10.1080/03235408.2018.1557915

Dania, V.O. (2019). Bioefficacy of Trichoderma species against important fungal pathogens causing post-harvest rot in sweet potato (Ipomoea batatas (L.) Lam). Journal of Bangladesh Agricultural University, 17(4), 446–453. https://doi.org/10.3329/jbau.v17i4.44604

Diba, K., Kordbacheh, P., Mirhendi, SH., Rezaie, S., & Mahmoudi, M. (2007). Identification of Aspergillus species using morphological characteristics. Pakistan Journal of Medical Science, 23(6), 867-872.

Divakara, S. T., Aiyaz, M., Hariprasad, M., Nayaka, S. C., & Niranjana, S. R. (2014). Aspergillus flavus infection and aflatoxin contamination in sorghum seeds and their biological management. Archives of Phytopathology and Plant Production 47(17), 2141-2156. https://doi.org/10.1080/03235408.2013.869892

FAOSTAT. (2017). FAOSTAT, Statistical data base. Rome: Food and Agricultural Organizations of the United Nations. Retrieved online on August 18, 2018 from www.faostat.org

Gachomo, E. W., & Kotchoni, O. S. (2008). The use of Trichoderma harzianum and T. viride as potential biocontrol agents against peanut microflora and their effectiveness in reducing aflatoxin contamination of infected kernels. African Journal of Biotechnology, 7, 439-447. http://doi103923/biotech.2008.439.447.

Gaiottia F., Marcuzzoa, P., Belfiorea, N., Lovata, L., Fornasier, F., & Tomasia, D. (2017). Influence of compost addition on soil properties, root growth and vine performances of Vitis vinifera cv Cabernet sauvignon. Scientia Horticulturae, 225,88–95. DOI: 10.1016/j.scienta.2017.06.052

Gams, W., Christensen, M., Onions, A.H., Pit, J.I., & Samson, R.A. (1985). Infrageneric taxa of Aspergillus. In: Advances in Penicillium and Aspergillus systematics, Samson, R.A., and Pit, J.I., eds. (New York, USA: Plenum Press), pp. 55–62.

Gomez, K.A. and Gomez, A. 1984. Statistical procedure for agricultural research. 2ndedn. John Wiley and Sons Inc. New York. 740p.

Guo, B., Chen, Z. Y., Lee, R. D., & Scully, B. T. (2008). Drought stress and pre-harvest aflatoxin contamination in agricultural commodity: Genetics, Genomics and Proteomics. Journal of International Plant Biology, 50:1281-1291. Doi: 10.1111/j.1744-7909.2008.00739.x.

Guru, P. R. 2014. Detection and sustainable management of aflatoxin contamination in chilli and groundnut. Ph.D Dissertation. Dept. of Plant Pathology, University of Agricultural Sciences, Raichur. 230pp.

Hamidou, F., Rathore, A., Waliyar, F., & Vadez, V. (2014). Although drought intensity increases aflatoxin contamination, drought tolerance does not lead to less aflatoxin contamination. Field Crops Research, 156, 103–110. https://doi.org/10.1016/j.fcr.2013.10.019

Hermosa, R., Viterbo,A., Chet, I., & Monte, E. (2012). Plant-beneficial effects of Trichoderma and of its genes. Microbiology 158, 17-25. http://doi 10.1099/mic.0.052274-0.

International Institute of Tropical Agriculture (IITA). (2018). Instructions for aflasafe application in the field. Accessed online on September 8, 2018 at www.iita.org

Karthikeya, Sankaralingam, A., & Nakkeeran, S. (2006) Management of groundnut root rot with biocontrol agents and organic amendments. Archives Of Phytopathology And Plant Protection, 39(3), 215-223, http://10.1080/03235400500094225

Khalid, S. (2017). Trichoderma as biological control weapon against soil-borne plant pathogens. African Journal of Biotechnology, 16.50:22-30. https://doi.org/10.5897/AJB2017.16270

Kifle, M. H., Yobo, K. S., & Laing, M. D. (2016). Biocontrol of Aspergillus flavus in groundnut using Trichoderma harzianum stain kd. Journal of Plant Diseases and Protection, 124(1), 51–56. Doi:10.1007/s41348-016-0066-4.

Klich, M.A. (2002). Identification of common Aspergillus Species; Centraalbureau voor Schimmelcultures: Utrecht, The Netherlands, pp. 426–432.

Kumar, S., Thakur, M., & Rani, A. (2014). Trichoderma: Mass production, formulation, quality control, delivery and its scope in commercialization in India for the management of plant diseases. African Journal of Agricultural Research, 9(53), 3838-3852. https://doi.org/10.5897/AJAR2014. 9061

McClenny, N, (2005). Laboratory detection and identification of Aspergillus species by microscopic observation and culture: The traditional approach. Journal of Medical and Veterinary Mycology, 43(1), S125-S128. https://doi.org/10.1080/13693780500052222

McClintock, N.C., & Diop, A.M. (2005). Soil fertility management and compost use in senegal’s peanut basin. International Journal of Agricultural Sustainability, 3,79–91. https://doi 10.1080/14735903.2005.9684746

Moya, P., Girotti, J., Toledo, A.. & Sisterna, M. (2018). Antifungal activity of Trichoderma VOCs against Pyrenophoea teres,the causal agent of barley net blotch. Journal of Plant Protection Research, 58(1), 45-53. DOI: https://doi.org/10.24425/119115

Parimi, V., Kotamraju, V., & Sudini, H. (2018). On-farm demonstrations with a set of good agricultural practices (GAPs) proved cost-effective in reducing pre-harvest aflatoxin contamination in groundnut. Agronomy, 8(2), 10. http://doi:10.3390/agronomy8020010.

Patila, V. P., Kawde, R. V., Khurdade, S. D., Devdhe, S. J., Kale, S. H., Patil, A. A., & Wakte, P. S. (2013). Development and validation of HPTLC method for simultaneous analysis of tramadol HCl and paracetamol in fixed-dose combination tablets. Journal of Indian Chemical Society, 90(1), 745-750.

Rai, S. (2017). Genetic diversity of antagonistic Trichoderma species against phytopathogens of Lycopersicon esculentum Mill. A Ph.D dissertation submitted to the Department of Microbiology and Fermentation Technology. Jacob school of Biotechnology and Bioengineering, India 18-30.

Redda, E., Ma, J., Mei, J., Li, M., Wu, B., & Jiang, X. (2018). Antagonistic potential of different isolates of Trichoderma against Fusarium oxysporum, Rhizoctonia solani and Botrytis cinerea. European Journal of Experimental Biology, 8(2), 12-18. http://doi 0.21767/2248-9215.100053

SAS Institute Inc. 2009. What’s new in SAS® 9.2. Cary, NC: SAS Institute Inc

Sekhar, I., Khayum, A., Prasad, T., & Sarada, J. (2017). Identification of Trichoderma species based on morphological characters isolated from rhizosphere of groundnut (Arachis hypogaea L.) International Journal of Science, Environment and Technology, 6 (3), 56-63.

Shifa, H., Tasneem, T., Gopalakrishnan, C., & Velazhahan, R. (2016)

Biological control of pre-harvest aflatoxin contamination in groundnut (Arachis hypogaea L.) with Bacillus subtilis G1, Archives of Phytopathology and Plant Protection, http://doi 10.1080/03235408.2016.1160642

Siddiquee, S., Cheong, B., Taslima, K., Kausar, H. and Hasan, M. 2012. Separation and identification of volatile organic compounds from liquid cultures of Trichoderma harzianum by GC-MS using three different capillary columns. Journal of Chromatographic Sciences, 50, 358 – 367. http:// doi 10.1093/chromsci/bms012

Sudini, H., Ranga Rao, G.V., Gowda, C.L.L., Chandrika, R., Margam, V., Rathore, A., & Murdock, L.L. (2015). Purdue improved crop storage (PICS) bags for safe storage of groundnuts. Journal of Stored Products Research, 64, 133–138. http://dx.doi.org/10.1016/j.jspr.2014.09.002 0

Thathana , M.G., Murage, H., Abia, A.L.K., & Pillay, M. (2017). Morphological characterization and determination of aflatoxin-production potentials of Aspergillus flavus isolated from maize and soil in Kenya. Agriculture, 7, 1-14. https://doi.org/10.3390/agriculture7100080

Torres, A., Barros, G., Palacios, S., Chulze, S., & Battilani, P. (2014). Review on pre-and post-harvest management of peanuts to minimize aflatoxin contamination. Food Research International 62, 11.-19. http:// doi 10.1016/j.foodres.2014.02.023

Vipul, K., Mohammad, S., Muksesh, S, Sonika, P., & Anuradha, S. (2014). Role of secondary metabolites produced by commercial Trichoderma species and their effect against soil borne pathogens. Journal of Biological Science, 3(8), 4-10. http://doi 10.4172/2090-4967.1000108

Wacoo, A. P., Wendiro, D., Vuzi, P. C., & Hawumba, J. F. (2014). Methods for detection of aflatoxins in agricultural food crops. Journal of Applied Chemistry 7(6), 29-46. https://doi.org/10.1155/2014/706291

Waliyar F., Osiru, M., Sudini, H.K., & Njoroge, SM.C. (2013). Reducing aflatoxins in groundnuts through integrated management and biocontrol. In: L Unnevehr and D. Grace (Eds). Aflatoxins: Finding solutions for improved food safety. International Food Policy Research Institute, 2033 K Street, NW, Washington, DC 20006-1002, USA

Waliyar, F., Osiru, M., Ntare, B.R., Kumar, K., Sudini, H., Traore, A., & Diarra, B. (2015). Post-harvest management of aflatoxin contamination in groundnut. World Mycotoxin Journal 8 (2): 245 – 252. http:// doi oar.icrisat.org/id/eprint/7389.

Woo, S., Ruocco, M., Vinale, F., Nigro, M., Marra, R., Lombardi, N., Pascale, A., Lanzuise, S., Manganiello, G., & Lorito, M. (2014). Trichoderma-based products and their widespread use in agriculture. Journal of Mycology, 8, 71-126. http://doi 10.2174/1874437001408010071




DOI: http://doi.org/10.17503/agrivita.v42i3.2670

Copyright (c) 2020 Victor Ohileobo Dania

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.