Structure and Composition of Major Arbuscular Mycorrhiza (MA) under Different Farmer Management of Coffee and Pine Agroforestry System

Cahyo Prayogo, Dimas Prastyaji, Budi Prasetya, Novi Arfarita


Utilization of Arbuscular Mycorrhiza (MA) as beneficial soil microbes is expected to support nutrient demand for improving crop performance. However, under the agroforestry system that facing a problem on light, water, and nutrients competitions, the role of MA is becoming unclear. The purpose of this research was to examine how far different management in Coffee Pines Agroforestry System (CPAS) affects MA structure and compositions. The relationship between soil parameters (e.g. pH, soil organic C) and MA activities was also being evaluated. The selected plot according to existing management practices were chosen as follows: (1) Low management (LC); (2) High management which then compared to (3) Business As Usual (BAU) plot in which were repeated in triplicate. ANOVA and multivariate analysis were employed to determine the effect of the treatments. The result showed that there were significant differences (p<0.05) in the structure and composition of the MA, in terms of the total number of MA spores and the abundance of Glomus sp. under the coffee tree sampling point, while the lowest number was detected in Gigaspora sp. genera. The more intensive land management resulted in a higher abundance of MA biomass which then leads to increased soil P and uptake-P along with MA infection.


Biological indicator; Land management; MA infection; Nutrient uptake; Soil microorganism

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Al Qassam, I., & Prayogo, C. (2018). Hubungan pendugaan cadangan karbon pada tegakan jati menggunakan penginderaan jauh dan pengukuran lapangan. Jurnal Tanah Dan Sumberdaya Lahan, 5(1), 727–737. Retrieved from website

Anggreiny, Y., Nazip, K., & Santri, D. J. (2017). Identifikasi fungi mikoriza arbuskula (FMA) pada rhizosfir tanaman di kawasan revegetasi lahan penambangan timah di Kecamatan Merawang Kabupaten Bangka dan sumbangannya pada pembelajaran biologi SMA. In Prosiding Seminar Nasional Pendidikan IPA 2017: STEM untuk Pembelajaran SAINS Abad 21 (pp. 391–403). Palembang, ID: Universitas Sriwijaya. Retrieved from website

Atmojo, S. W. (2003). Peranan bahan organik terhadap kesuburan tanah dan upaya pengelolaannya. Sebelas Maret University Press. Retrieved from pdf

Aučina, A., Rudawska, M., Leski, T., Skridaila, A., Riepšas, E., & Iwanski, M. (2007). Growth and mycorrhizal community structure of Pinus sylvestris seedlings following the addition of forest litter. Applied and Environmental Microbiology, 73(15), 4867–4873. crossref

Augé, R. M., Toler, H. D., & Saxton, A. M. (2016). Mycorrhizal stimulation of leaf gas exchange in relation to root colonization, shoot size, leaf phosphorus and nitrogen: A quantitative analysis of the literature using meta-regression. Frontiers in Plant Science, 7, 1084. crossref

Bakhshipour, R., Kahneh, E., & Khah, A. A. (2009). Effect of land use change on status of arbuscular mycorrhizal fungi in some hyrcanian forest soil a case study of Lahidjan Region, Iran. Asian Journal of Chemistry, 21(3), 1685–1689. Retrieved from website

Bücking, H., Liepold, E., & Ambilwade, P. (2012). The role of the mycorrhizal symbiosis in nutrient uptake of plants and the regulatory mechanisms underlying these transport processes. In N. K. Dhal & S. C. Sahu (Eds.), Plant Science. IntechOpen. crossref

Ceccarelli, N., Curadi, M., Martelloni, L., Sbrana, C., Picciarelli, P., & Giovannetti, M. (2010). Mycorrhizal colonization impacts on phenolic content and antioxidant properties of artichoke leaves and flower heads two years after field transplant. Plant and Soil, 335, 311–323. crossref

Chen, Y. L., Liu, R. J., Bi, Y. L., & Feng, G. (2014). Use of mycorrhizal fungi for forest plantations and minesite rehabilitation. In Z. Solaiman, L. Abbott, & A. Varma (Eds.), Mycorrhizal Fungi: Use in Sustainable Agriculture and Land Restoration (pp. 325–355). Berlin, Heidelberg: Springer. crossref

Daras, U., Sobari, I., Trisilawati, O., & Towaha, J. (2015). Pengaruh mikoriza dan pupuk NPKMg terhadap pertumbuhan dan produksi kopi arabika. Jurnal Tanaman Industri Dan Penyegar, 2(2), 91–98. crossref

Daras, U., Trisilawati, O., & Sobari, I. (2013). Pengaruh mikoriza dan amelioran terhadap pertumbuhan benih kopi. Jurnal Tanaman Industri Dan Penyegar, 4(2), 145–156. Retrieved from website

Davison, J., Öpik, M., Daniell, T. J., Moora, M., & Zobel, M. (2011). Arbuscular mycorrhizal fungal communities in plant roots are not random assemblages. FEMS Microbiology Ecology, 78(1), 103–115. crossref

Davison, J., Öpik, M., Zobel, M., Vasar, M., Metsis, M., & Moora, M. (2012). Communities of arbuscular mycorrhizal fungi detected in forest soil are spatially heterogeneous but do not vary throughout the growing season. PLoS ONE, 7(8), e41938. crossref

Duffy, P. J. (2014). Vegetation and soil characteristics of pine plantations and naturally regenerated hardwood forests on the Hoosier National Forest. Purdue University. Retrieved from website

Dumbrell, A. J., Nelson, M., Helgason, T., Dytham, C., & Fitter, A. H. (2010). Relative roles of niche and neutral processes in structuring a soil microbial community. ISME Journal, 4, 337–345. crossref

Gao, Q. M., & Guo, L. D. (2010). A comparative study of arbuscular mycorrhizal fungi in forest, grassland and cropland in the Tibetan Plateau, China. Mycology, 1(3), 163–170. crossref

Garcés-Ruiz, M., Senés-Guerrero, C., Declerck, S., & Cranenbrouck, S. (2017). Arbuscular mycorrhizal fungal community composition in Carludovica palmata, Costus scaber and Euterpe precatoria from weathered oil ponds in the Ecuadorian Amazon. Frontiers in Microbiology, 8, 2134. crossref

Gommers, C. M. M., Visser, E. J. W., Onge, K. R. S., Voesenek, L. A. C. J., & Pierik, R. (2013). Shade tolerance: When growing tall is not an option. Trends in Plant Science, 18(2), 65–71. crossref

Hartoyo, B. (2012). Efektivitas fungi mikoriza arbuskula pada penggunaan pupuk fosfor alami dan pengaruhnya terhadap pertumbuhan, biomassa dan produksi asiatikosida pegagan (Centella asiatica L. Urban) di andosol. IPB University. Retrieved from website

Hartoyo, B., Gulamahdi, M., Darusman, L. K., Aziz, S. A., & Mansur, I. (2011). Keanekaragaman fungi mikoriza arbuskula (FMA) pada rizosfer tanaman pegagan (Centella asiatica (L.) Urban). Jurnal Penelitian Tanaman Industri, 17(1), 32–40. crossref

Herianto, Kusuma, Z., Nihayati, E., & Prayogo, C. (2019). Estimation of carbon stocks in residual stands area, Central Kalimantan. Ecology, Environment and Conservation, 25(1), 234–241. Retrieved from website

Hidayati, N., Faridah, E., & Sumardi. (2015). Peran mikoriza pada semai beberapa sumber benih mangium (Acacia mangium Willd.) yang tumbuh pada tanah kering. Jurnal Pemuliaan Tanaman Hutan, 9(1), 13–15. crossref

Hoeksema, J. D., Chaudhary, V. B., Gehring, C. A., Johnson, N. C., Karst, J., Koide, R. T., … Umbanhowar, J. (2010). A meta-analysis of context-dependency in plant response to inoculation with mycorrhizal fungi. Ecology Letters, 13(3), 394–407. crossref

Ibiremo, O. S., Daniel, M. ., Oloyede, A. ., & Iremiren, G. O. (2011). Growth of coffee seedlings as influenced by arbuscular mycorrhizal inoculation and phosphate fertilizers in two soils in Nigeria. International Research Journal of Plant Science, 2(6), 160–166. Retrieved from website

Indriani, N. P., Mansyur, Susilawati, I., & Islami, R. Z. (2011). Peningkatan produktivitas tanaman pakan melalui pemberian fungi mikoriza arbuskular (FMA). Pastura, 1(1), 27–30. Retrieved from website

Jansa, J., Finlay, R., Wallander, H. ̊kan, Smith, F. A., & Smith, S. E. (2011). Role of mycorrhizal symbioses in phosphorus cycling. In E. Bünemann, A. Oberson, & E. Frossard (Eds.), Phosphorus in Action (Vol. Soil Biolo, pp. 137–168). Berlin, Heidelberg: Springer. crossref

Jehne, W., & Lee, P. (2014). The role of mycorrhizal fungi in regenerating healthy soils and agricultural productivity. Nedlands WA, Australia. Retrieved from website

Jung, S. C., Martinez-Medina, A., Lopez-Raez, J. A., & Pozo, M. J. (2012). Mycorrhiza-induced resistance and priming of plant defenses. Journal of Chemical Ecology, 38(6), 651–664. crossref

Knegt, B., Jansa, J., Franken, O., Engelmoer, D. J. P., Werner, G. D. A., Bücking, H., & Kiers, E. T. (2016). Host plant quality mediates competition between arbuscular mycorrhizal fungi. Fungal Ecology, 20, 233–240. crossref

Koorem, K., Gazol, A., Öpik, M., Moora, M., Saks, Ü., Uibopuu, A., … Zobel, M. (2014). Soil nutrient content influences the abundance of soil microbes but not plant biomass at the smallscale. PLoS ONE, 9(3), e91998. crossref

Koorem, K., Tulva, I., Davison, J., Jairus, T., Öpik, M., Vasar, M., … Moora, M. (2017). Arbuscular mycorrhizal fungal communities in forest plant roots are simultaneously shaped by host characteristics and canopy-mediated light availability. Plant and Soil, 4(10), 259–271. crossref

Lebrón, L., Lodge, D. J., & Bayman, P. (2012). Differences in arbuscular mycorrhizal fungi among three coffee cultivars in Puerto Rico. ISRN Agronomy, 2, 148–155. crossref

Lekberg, Y., & Koide, R. T. (2013). Integrating physiological, community, and evolutionary perspectives on the arbuscular mycorrhizal symbiosis. Botany, 92(4), 241-251. crossref

Liu, Y., Mao, L., Li, J., Shi, G., Jiang, S., Ma, X., … Feng, H. (2014). Resource availability differentially drives community assemblages of plants and their root-associated arbuscular mycorrhizal fungi. Plant and Soil, 386, 341–355. crossref

Melo, C. D., Walker, C., Krüger, C., Borges, P. A. V., Luna, S., Mendonça, D., … Machado, A. C. (2019). Environmental factors driving arbuscular mycorrhizal fungal communities associated with endemic woody plant Picconiaazorica on native forest of Azores. Annals of Microbiology, 69, 1309–1327. crossref

Miska, M. E. E., Junaedi, A., Wachjar, A., & Mansur, I. (2016). Karakterisasi fungi mikoriza arbuskula pada rhizosfer aren (Arenga pinnata (Wrmb) Merr.) dari Jawa Barat dan Banten. Jurnal Silvikultur Tropika, 7(1), 18–23. Retrieved from website

Moora, M., Davison, J., Öpik, M., Metsis, M., Saks, Ü., Jairus, T., … Zobel, M. (2014). Anthropogenic land use shapes the composition and phylogenetic structure of soil arbuscular mycorrhizal fungal communities. FEMS Microbiology Ecology, 90(3), 609–621. crossref

Muryati, S., Mansur, I., & Budi, S. W. (2016). Keanekaragaman fungi mikoriza arbuskula (FMA) pada rhizosfer Desmodium spp. asal PT. Cibaliung Sumberdaya, Banten. Jurnal Silvikultur Tropika, 7(3), 188–197. Retrieved from website

Neuenkamp, L., Zobel, M., Lind, E., Gerz, M., & Moora, M. (2019). Arbuscular mycorrhizal fungal community composition determines the competitive response of two grassland forbs. PLoS ONE, 14(7), e0219527. crossref

Pozo, M. J., López-Ráez, J. A., Azcón-Aguilar, C., & García-Garrido, J. M. (2015). Phytohormones as integrators of environmental signals in the regulation of mycorrhizal symbioses. New Phytologist, 205(4), 1431–1436. crossref

Prayogo, C., Sari, R. R., Asmara, D. H., Rahayu, S., & Hairiah, K. (2018). Allometric equation for pinang (Areca catechu) biomass and C stocks. AGRIVITA Journal of Agricultural Science, 40(3), 381–389. crossref

Prayogo, C., Sholehuddin, N., Putra, E. Z. H. S., & Rachmawati, R. (2019). Soil macrofauna diversity and structure under different management of pine-coffee agroforestry system. Journal of Degraded and Mining Lands Management, 6(3), 1727–1736. crossref

Ramadhan, R., Nihayati, E., & Sitawati. (2017). Pengaruh aplikasi cendawan mikoriza dan perlakuan pemberian air terhadap peningkatan kadar asiatikosida tanaman pegagan (Centella asiatica (L) urban). Biotropika, 5(3), 138-142. crossref

Sarah, S., & Ibrar, M. (2016). Effects of arbuscular mycorrhizal fungi on spores density and root colonization of four hybrids of sunflower (Helianthus annuus L.) at different rock phosphate levels. Sarhad Journal of Agriculture, 32(4), 258-266. crossref

Sari, M. N., Sudarsono, & Darmawan. (2017). Pengaruh bahan organik terhadap ketersediaan fosfor pada tanah-tanah kaya Al dan Fe. Buletin Tanah Dan Lahan, 1(1), 65–71. Retrieved from website

Shi, G., Liu, Y., Johnson, N. C., Olsson, P. A., Mao, L., Cheng, G., … Feng, H. (2014). Interactive influence of light intensity and soil fertility on rootassociated arbuscular mycorrhizal fungi. Plant and Soil, 378, 173–188. crossref

Smith, S., & Read, D. (2008). Mycorrhizal symbiosis (3rd ed.). Academic Press. Retrieved from website

Soka, G. E., & Ritchie, M. E. (2016). Land-cover legacy effects on arbuscular mycorrhizal abundance in human and wildlife dominated systems in tropical savanna. Advances in Ecology, 2016, 1–11. crossref

Solaiman, Z. M., Abbott, L. K., & Varma, A. (2014). Mycorrhizal fungi: Use in sustainable agriculture and land restoration. Soil Biology Volume 41. Berlin, Heidelberg: Springer. crossref

Songachan, L. S., & Kayang, H. (2011). Diversity of arbuscular mycorrhizal fungi in pine forest of Meghalaya, North East India. Mycosphere, 2(4), 497–505. Retrieved from pdf

Suamba, I. W., Wirawan, I. G. P., & Adiartayasa, W. (2014). Isolasi dan identifikasi fungi mikoriza arbuskular (FMA) secara mikroskopis pada rhizosfer tanaman jeruk (Citrus sp.) di Desa Kerta, Kecamatan Payangan, Kabupaten Gianyar. Jurnal Agroekoteknologi Tropika, 3(4), 201–208. Retrieved from website

Sukmawaty, E., Hafsan, & Asriani. (2016). Identifikasi cendawan mikoriza arbuskula dari perakaran tanaman pertanian. Biogenesis: Jurnal Ilmiah Biologi, 4(1), 16–20. crossref

Suprayogo, D., Azmi, E. N., Ariesta, D. A., Sutejo, Y. A., Hakim, A. L., Prayogo, C., & McNamara, N. P. (2020). Tree and plant interactions in the agroforestry system: Does the management of coffee intensification disrupt the soil hydrological system and pine growth? IOP Conference Series: Earth and Environmental Science, 449, 012045. crossref

Trevizan Chiomento, J. L., Stürmer, S. L., Carrenho, R., Castoldi da Costa, R., Meredith SchefferBasso, S., Corrêa Antunes, L. E., … Oliveira Calvete, E. (2019). Composition of arbuscular mycorrhizal fungi communities signals generalist species in soils cultivated with strawberry. Scientia Horticulturae, 253, 286–294. crossref

Ura’, R., Paembonan, S. A., & Umar, A. (2015). Karakteristik fungi arbuskular mikoriza genus Glomus pada akar beberapa jenis pohon di Hutan Kota Universitas Hasanuddin Tamalanrea. Jurnal Alam Dan Lingkungan, 6(11), 16–21. Retrieved from website

Wanda, A. R., Yuliani, & Trimulyono, G. (2015). Keanekaragaman cendawan mikoriza vesikular arbuskular (MVA) di hutan pantai Nepa Sampang Madura berdasarkan gradien salinitas. Lentera Bio, 4(3), 180–186. Retrieved from website

Yang, A. N., Lu, L., & Zhang, N. (2011). The diversity of arbuscular mycorrhizal fungi in the subtropical forest of Huangshan (Yellow Mountain), EastCentral China. World Journal of Microbiology and Biotechnology, 27, 2351–2358. crossref

Zhu, X., Yang, W., Song, F., & Li, X. (2020). Diversity and composition of arbuscular mycorrhizal fungal communities in the cropland black soils of China. Global Ecology and Conservation, 22, e00964. crossref


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