Rhodospirillum centenum, A New Growth Stimulant and Antagonistic Bacteria Against Leaf Spot of Rice Caused by Curvularia lunata

Rungrat Vareeket, Kasem Soytong

Abstract


The research objectives were to find Rhodospirillum spp. to promote plant growth and as antagonists to control leaf spot of rice. The results showed that rice var. RD41 and Pitsanulok 2 (PL2) had leaf spot caused by Curvularia lunata. R. centenum is gram negative, mobile cell motility and negative of gelatin activity test. SM41 and 61 showed glucose and lactose and/or sucrose fermentation activity, but SM72 and 92 were non-fermented activity. Isolates of R. centenum were antagonized C. lunata, a leaf spot pathogen. R. centenum expressed for ability to produce amylase, protease and lipase. R. centenum isolates SM41, SM61, SM72, and SM92 with C. lunata causing leaf spot and seed-borne fungus. In addition, the inoculated seeds var. RD 41 and Pitsanulok 2 (PL2) applied R. centenum 41, 61, 72 and 79 gave significantly better seed germination, shoot and root length. Isolate SM41 reduced disease incidence in rice var. RD41 which the disease index was on level 2 when the inoculated one was level 5. Isolate SM61 showed the disease index of level 2 but the inoculated control was level 5. It is the first report that R. centenum plays the role for growth stimulant and biological control agents against leaf spot of rice caused by C. lunata.

Keywords


Curvularia lunata; Growth stimulate; Leaf spot of rice; Photosynthesizing bacteria; Rice

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References


Anderson, L. E., & Fuller, R. C. (1969). Photosynthesis in Rhodospirillum rubrum. IV. Isolation and charactherization of ribulose 1,5-diphosphate carboxylase. The Journal of Biological Chemistry, 244(12), 3105–3109. Retrieved from PDF

Berleman, J. E., & Bauer, C. E. (2004). Characterization of cyst cell formation in the purple photosynthetic bacterium Rhodospirillum centenum. Microbiology, 150(2), 383–390. DOI

Domsch, K. H., Gams, W., & Anderson, T.-H. (1980). Compendium of soil fungi (Vol. 1). London: Academic Press.

Fakhrunnisa, Hashmi, M. H., & Ghaffar, A. (2006). Seedborne mycoflora of wheat, sorghum and barley. Pakistan Journal of Botany, 38(1), 185–192. Retrieved from PDF

Harrigan, W. F., & McCance, M. E. (1976). Laboratory methods in food and dairy microbiology. London: Academic Press Inc.

Imhoff, J. F., & Trüper, H. G. (1989). The purple nonsulfur bacteria. In J. T. Staley, M. P. Bryant, N. Pfennig, & J. G. Holt (Eds.), Bergey’s Manual of Systematic Bacteriology (Vol. 3) (pp. 1658-1661). Baltimore: Wiiliams and Wilkins.

Jiang, Z.-Y., Rushing, B. G., Bai, Y., Gest, H., & Bauer, C. E. (1998). Isolation of Rhodospirillum centenum mutants defective in phototactic colony motility by transposon mutagenesis. Journal of Bacteriology, 180(5), 1248–1255. DOI

Kim, M. K., Choi, K.-M., Yin, C.-R., Lee, K.-Y., Im, W.-T., Lim, J. H., & Lee, S.-T. (2004). Odorous swine wastewater treatment by purple non-sulfur bacteria, Rhodopseudomonas palustris, isolated from eutrophicated ponds. Biotechnology Letters, 26, 819–822. DOI

Kohlmiller Jr., E. F., & Gest, H. (1951). A comparative study of the light and dark fermentations of organic acids by Rhodospirillum rubrum. Journal of Bacteriology, 61(3), 269–282. DOI

Lu, Y.-K., Marden, J., Han, M., Swingley, W. D., Mastrian, S. D., Chowdhury, S. R., … Touchman, J. W. (2010). Metabolic flexibility revealed in the genome of the cyst-forming α-1 proteobacterium Rhodospirillum centenum. BMC Genomics, 11, 325. DOI

Manandhar, H. K., Jørgensen, H. J. L., Mathur, S. B., & Smedegaard-Petersen, V. (1998). Suppression of rice blast by preinoculation with avirulent Pyricularia oryzae and the nonrice pathogen Bipolaris sorokiniana. Phytopathology, 88(7), 735–739. DOI

Pitt, J. I., & Hocking, A. D. (1997). Primary keys and miscellaneous fungi. In Fungi and Food Spoilage (pp. 59–171). Boston, MA: Springer. DOI

Pitt, J. I., Hocking, A. D., Bhudhasamai, K., Miscamble, B. F., Wheeler, K. A., & Tanboon-Ek, P. (1994). The normal mycoflora of commodities from Thailand. 2. Beans, rice, small grains and other commodities. International Journal of Food Microbiology, 23(1), 35–43. DOI

Rana, G., Meikap, S., Mondol, M., Bose, P. P., & Mandal, T. (2016). Green-fertilizer, Rhodospirillum rubrum, for agricultural development on flyash without any toxic metal ion release. Basic Research Journal of Agricultural Science and Review, 5(8), 109–117. Retrieved from website

Sambrook, J., Fritsch, E. F., & Maniatis, T. (1989). Molecular cloning: A laboratory manual (2nd ed.). New York: Cold Spring Harbor Laboratory Press.

Serdyuk, O. P., Smolygina, L. D., Kobzar, E. F., & Gogotov, I. N. (1993). Occurrence of plant hormones in cells of the phototrophic purple bacterium Rhodospirillum rubrum 1R. FEMS Microbiology Letters, 109(1), 113–116. DOI

Smith, B. D. (1998). The emergence of agriculture. New York: Scientific American Library.

Song, F., & Goodman, R. M. (2001). Molecular biology of disease resistance in rice. Physiological and Molecular Plant Pathology, 59(1), 1–11. DOI

Soytong, K. (1992). Antagonism of Chaetomium cupreum to Pyricularia oryzae. Journal of Plant Protection in the Tropics, 9(1), 17–23. Retrieved from website

Tadesse, I., Isoaho, S. A., Green, F. B., & Puhakka, J. A. (2003). Removal of organics and nutrients from tannery effluent by advanced integrated wastewater pond systems technology. Water Science and Technology, 48(2), 307–314. DOI

Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729. DOI

Tann, H., & Soytong, K. (2016). Bioformulations and nano product from Chaetomium cupreum CC3003 to control leaf spot of rice var. Sen Pidoa in Cambodia. International Journal of Plant Biology, 7(1), 59–63. DOI

Tann, H., & Soytong, K. (2017). Biological control of brown leaf spot disease caused by Curvularia lunata and field application method on rice variety IR66 in Cambodia. AGRIVITA Journal of Agricultural Science, 39(1), 111–117. DOI

Thavong, P. (2002). Effect of dirty panicle disease on rice seed vigor. Thai Agricultural Research Journal, 20(2), 111–120. Retrieved from website

Tongon, R., & Soytong, K. (2015). Application of nanoparticles from Chaetomium globosum to control leaf spot of rice. Journal of Agricultural Technology, 11(8), 1919–1926. Retrieved from PDF

Vareeket, R., & Soytong, K. (2017). Screening of antagonistic bacteria for biological control of rice diseases. International Journal of Agricultural Technology, 13(7.3), 2569–2573. Retrieved from website

Veenstra, S., Al-Nozaily, F. A., & Alaerts, G. J. (1995). Purple non-sulfur bacteria and their influence on waste stabilisation pond performance in the Yemen Republic. Water Science and Technology, 31(12), 141–149. DOI




DOI: http://doi.org/10.17503/agrivita.v41i0.2181

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