Evaluation of Cladosporium sp. Mycoparacites as Biocontrol Agents of White Rust Disease on Chrysanthemum

Evi Silvia Yusuf, Kurniawan Budiarto, Indijarto Budi Rahardjo

Abstract


Cladosporium is a promising mycoparasite to control white rust disease in chrysanthemum. The research was conducted to evaluate several collected Cladosporium isolates against P. horiana, their parasitism mechanism and population dynamic in a production cycle. The experiment was carried out at the Indonesian Ornamental Crops Research Institute (IOCRI) from January to December 2015. Ten Cladosporium isolates with two synthetic fungicides and one negative control were designedly sprayed on chrysanthemum cv White Fiji. The results showed that Cladosporium isolates gave varied suppression to white rust intensity. Isolates HNC, DC, SGC and UC had higher parasitism and suppression intensities than other isolates and their effectiveness was the same as synthetic fungicides. Longer flower vase life was produced from Cladosporium and synthetic fungicide treated plants, while flower stalk and diameter were less affected by the Cladosporium applications. The population dynamic of Cladosporium was accordingly responded with the rust intensity in Cladosporium-treated plants. The parasitizing mechanism of Cladosporium to P. horiana was through teliospores envelopment resulted in the changes of teliospores morphology.

Keywords


Chrysanthemum; Cladosporium; Mycoparasite; Puccinia horiana

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References


Abdel-Rahim, I. R., & Abo-Elyousr, K. A. M. (2018). Talaromyces pinophilus strain AUN-1 as a novel mycoparasite of Botrytis cinerea, the pathogen of onion scape and umbel blights. Microbiological Research, 212, 1–9.

Alaei, H., De Backer, M., Nuytinck, J., Maes, M., Höfte, M., & Heungens, K. (2009). Phylogenetic relationships of Puccinia horiana and other rust pathogens of Chrysanthemum × morifolium based on rDNA ITS sequence analysis. Mycological Research, 113(6–7), 668–683. crossref

Assante, G., Maffi, D., Saracchi, M., Farina, G., Moricca, S., & Ragazzi, A. (2004). Histological studies on the mycoparasitism of Cladosporium tenuissimum on urediniospores of Uromyees appendiculatus. Mycological Research, 108(2), 170–182. crossref

Baiswar, P., Chandra, S., & Kumar, R. (2008). First report of rust caused by Coleosporium plumeriae on Plumeria alba in India. Plant Pathology, 57(4), 787. crossref

Barros, S. T., Oliveira, N. T., Bastos, S. T. G., & Maia, L. C. (1999). Hyperparasitism of Cladosporium uredinicola over Puccinia puta on the host Ipomoea fistulosa. Mycologist, 13(1), 23–24. crossref

Bonde, M. R., Murphy, C. A., Bauchan, G. R., Luster, D. G., Palmer, C. L., Nester, S. E., … Berner, D. K. (2015). Evidence for systemic infection by Puccinia horiana, causal agent of chrysanthemum white rust, in chrysanthemum. Phytopathology, 105(1), 91–98. crossref

Busby, P. E., Peay, K. G., & Newcombe, G. (2016). Common foliar fungi of Populus trichocarpa modify Melampsora rust disease severity. New Phytologist, 209(4), 1681–1692. crossref

Ciancio, A., & Mukerji, K. G. (Eds.). (2008). Integrated management of diseases caused by fungi, phytoplasma and bacteria (1st ed.). Springer Netherlands. crossref

Dheepa, R., Renukadevi, P., Vinod Kumar, S., & Nakkeeran, S. (2015). First report of chrysanthemum white rust (Puccinia horiana) in India. Plant Disease, 99(9), 1279. crossref

García-Velasco, R., Zavaleta-Mejía, E., Rojas-Martínez, R. I., Leyva-Mir, S. G., Simpson, J. K., & Fuentes-Dávila, G. (2005). Antagonismo de Cladosporium sp. contra Puccinia horiana Henn. causante de la roya blanca del crisantemo (Dendranthema grandiflora Tzvelev). Revista Mexicana de Fitopatología, 23(1), 79–86. Retrieved from website

Göre, M. E. (2008). White rust outbreaks on chrysanthemum caused by Puccinia horiana in Turkey. Plant Pathology, 57(4), 786. crossref

Guimarães, J. B., Chambel, L., Melzoch, K., Pereira, P., & Tenreiro, R. (2011). Cladosporium sp. from phyloplane: a diversity evaluation on a Continental ecosystem. Mycosphere, 2(3), 191–201. Retrieved from PDF

Hanudin, Budiarto, K., & Marwoto, B. (2017). Application of PGPR and antagonist fungi-based biofungicide for white rust disease control and its economyc analysis in chrysanthemum production. AGRIVITA Journal of Agricultural Science, 39(3), 266–278. crossref

He, H., Ke, H., Keting, H., Qiaoyan, X., & Silan, D. (2013). Flower colour modification of chrysanthemum by suppression of F3′H and overexpression of the exogenous Senecio cruentus F3′5′H gene. PLoS ONE, 8(11), e74395. crossref

Herrera, C. S., Hirooka, Y., & Chaverri, P. (2016). Pseudocospeciation of the mycoparasite Cosmospora with their fungal hosts. Ecology and Evolution, 6(5), 1504–1514. crossref

James, T. Y., Marino, J. A., Perfecto, I., & Vandermeer, J. (2016). Identification of putative coffee rust mycoparasites via single-molecule DNA sequencing of infected pustules. Applied and Environmental Microbiology, 82(2), 631–639. crossref

Kjaer, K. H., & Ottosen, C.-O. (2011). Growth of chrysanthemum in response to supplemental light provided by irregular light breaks during the night. Journal of the American Society for Horticultural Science, 136(1), 3–9. crossref

Köhl, J. J., Molhoek, W. W. M. L., Groenenboom-De Haas, B. B. H., & Goossen-Van De Geijn, H. H. M. (2009). Selection and orchard testing of antagonists suppressing conidial production by the apple scab pathogen Venturia inaequalis. European Journal of Plant Pathology, 123(4), 401–414. crossref

Mims, C. W., & Richardson, E. A. (2006). Light and electron microscopic observations of Cladosporium sp growing on basidia of Exobasidium camelliae var. gracilis. Microscopy and Microanalysis, 12(S02), 460–461. crossref

Ministry of Agriculture Republic of Indonesia. (2016). Statistik pertanian 2016. Jakarta: Center for Agricultural Data and Information System, Ministry of Agriculture Republic of Indonesia. Retrieved from website

Morgan-Jones, G., & McKemy, J. (1990). Studies in the genus Cladosporium sensu lato. I. Concerning Cladosporium uredinicola, occuring on telial columns of Cronartium quercuum and other rusts. Mycotaxon, 39, 36849. Retrieved from website

Moricca, S., Ragazzi, A., & Assante, G. (2005). Biocontrol of rust fungi by Cladosporium tenuissimum. In M. H. Pei & A. R. McCracken (Eds.), Rust diseases of willow and poplar (pp. 213–229). CAB International. crossref

Moricca, Salvatore, Ragazzi, A., Mitchelson, K. R., & Assante, G. (2001). Antagonism of the two-needle pine stem rust fungi Cronartium flaccidum and Peridermium pini by Cladosporium tenuissimum in vitro and in planta. Phytopathology, 91(5), 457–468. crossref

Nikkels, A. H., Terstegge, P., & Spieksma, F. T. M. (1996). Ten types of microscopically identifiable airborne fungal spores at Leiden, The Netherlands. Aerobiologia, 12(2), 107-112. crossref

Plasmeijer, J., & Yanai, C. (2012). Cut flowers and ornamental plants. Market News Service: Floriculture Products. Retrieved from PDF

Rahardjo, I. B., Budiarto, K., & Marwoto, B. (2019). Evaluation of biological and chemical fungicides to control white rust in chrysanthemum grown under open condition. AGRIVITA, Journal of Agricultural Science, 41(1), 55–63.

Siegel-Hertz, K., Edel-Hermann, V., Chapelle, E., Terrat, S., Raaijmakers, J. M., & Steinberg, C. (2018). Comparative microbiome analysis of a Fusarium wilt suppressive soil and a Fusarium wilt conducive soil from the Chateaurenard region. Frontiers in Microbiology, 9, 1–16.

Srivastava, A. K., Défago, G., & Kern, H. (1985). Hyperparasitism of Puccinia horiana and other microcyclic rusts. Journal of Phytopathology, 114(1), 73–78. crossref

Suhardi. (2009). Sumber inokulum, respons varietas, dan efektivitas fungisida terhadap penyakit karat putih pada tanaman krisan. Jurnal Hortikultura, 19(2), 207–213. Retrieved from website

Torres, D. E., Rojas-Martínez, R. I., Zavaleta-Mejía, E., Guevara-Fefer, P., Márquez-Guzmán, G. J., & Pérez-Martínez, C. (2017). Cladosporium cladosporioides and Cladosporium pseudocladosporioides as potential new fungal antagonists of Puccinia horiana Henn., the causal agent of chrysanthemum white rust. PLoS ONE, 12(1), e0170782. crossref

Traquair, J. A., Meloche, R. B., Jarvis, W. R., & Baker, K. W. (1984). Hyperparasitism of Puccinia violae by Cladosporium uredinicola. Canadian Journal of Botany, 62(1), 181–184. crossref

Yusuf, E. S., Djatnika, I., & Suhardi. (2014). Koleksi dan karakterisasi mikoparasit asal karat putih pada krisan. Jurnal Hortikultura, 24(1), 56–64. Retrieved from website

Yusuf, E. S., Nuryani, W., & Hanudin. (2016). Isolasi dan identifikasi mikoparasit utama pada karat krisan. Jurnal Hortikultura, 26(2), 217–222. crossref

Zeng, J., Sun, J., Xu, Y., Chen, F., Jiang, J., Fang, W., & Chen, S. (2013). Variation for resistance to white rust (Puccinia horiana) among Ajania and Chrysanthemum species. HortScience, 48(10), 1231–1234. crossref

Zhan, G., Tian, Y., Wang, F., Chen, X., Guo, J., Jiao, M., … Kang, Z. (2014). A novel fungal hyperparasite of Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust. PLoS ONE, 9(11), e111484. crossref




DOI: http://doi.org/10.17503/agrivita.v41i3.1864

Copyright (c) 2019 Evi Silvia Yusuf

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