Kaempferia galanga L. A Medicinal Plant Used By Indonesian Ethnic Groups: Genetic Diversity Based on Inter-Simple Sequence Repeats (ISSR)

Dyah Subositi, Nina Kurnianingrum, Rohmat Mujahid, Yuli Widiyastuti

Abstract

khk
Kencur (Kaempferia galanga L.) has been recognized as a medicinal plant used by many ethnic groups in Indonesia. Up to present time, information on the diversity of the respected plant is still limited. The aim of this research was to reveal the genetic diversity of K. galanga in Indonesia as a baseline medicinal plant database. Inter-Simple Sequence Repeat (ISSR) molecular markers were employed to analyze the genetic diversity information of K. galanga from 12 selected locations in Indonesia where ethnic groups used the plant. Dice index similarity was used to calculate a similarity index, and Unweighted Pair Group Method Using Arithmetic Mean (UPGMA) was used to constructing a dendrogram. Ten selected ISSR primers generated 104 amplified DNA fragments, of which 98 fragments (94.2%) were polymorphic. The genetic similarity index among accessions ranged from 49.6–93.3%, indicating a high level of genetic diversity in K. galanga. The ISSR markers were thus able to genetically differentiate K. galanga accessions.


Keywords


Database; Genetic diversity; Inter-simple sequence repeats; Kaempferia galanga L.

Full Text:

PDF

References


Anjali, N., Ganga, K. M., Nadiya, F., Shefeek, S., & Sabu, K. K. (2016). Intraspecific variations in cardamom (Elettaria cardamomum Maton): assessment of genomic diversity by flow cytometry, cytological studies and ISSR analysis. SpringerPlus, 5(1), 1560. https://doi.org/10.1186/s40064-016-3226-x

Ashraf, K., Ahmad, A., Adnan Ali Shah, S., & Mujeeb, M. (2017). Genetic diversity in accessions of Indian turmeric (Curcuma longa L.) using RAPD markers. International Journal of Pharmacy and Pharmaceutical Sciences, 9(10), 288–291. https://doi.org/10.22159/ijpps.2017v9i10.18715

Bidyaleima, L., Kishor, R., & Sharma, G. J. (2019). Chromosome numbers, RAPD and ISSR profiles of six Zingiber species found in Manipur, India. Biodiversitas, 20(5), 1389–1397. https://doi.org/10.13057/biodiv/d200531

Blair, M. W., Panaud, O., & McCouch, S. R. (1999). Intersimple sequence repeat (ISSR) amplification for analysis of microsatellite motif frequency and fingerprinting in rice (Oryza sativa L.). Theoretical and Applied Genetics, 98(5), 780–792. https://doi.org/10.1007/s001220051135

Chaudhary, A. A., Dhar, U., Ahmad, A., Bhatt, I. D., Jugran, A., & Kaur, G. (2012). Analysis of genetic diversity in Hedychium spicatum Buch. Ham. ex. D. Don in West Himalaya, India. Journal of Medicinal Plants Research, 6(23), 3984–3991. Retrieved from https://academicjournals.org/journal/JMPR/article-full-text-pdf/340916C23327

Das, A., Gaur, M., Barik, D. P., & Subudhi, E. (2017). Genetic diversity analysis of 60 ginger germplasm core accessions using ISSR and SSR markers. Plant Biosystems, 151(5), 822–832. https://doi.org/10.1080/11263504.2016.1211197

Devi, K. D., Singh, S. B., Singh, N. S., Chingakham, B. S., Punyarani, K., & Devi, H. S. (2015). Evaluation of genetic relationships and chemical assay of Kaempferia galanga L. cultivars found in Manipur, North-East India. International Journal of Recent Scientific Research, 6(6), 4366–4373. Retrieved from https://www.recentscientific.com/sites/default/files/178.pdf

Ghosh, P., Shylaja, M. ., & Nazeem, P. A. (2015). RAPD marker based DNA fingerprinting in released varieties and selected superior somaclones of ginger (Zingiber officinale Rosc.). The Bioscan, 10(1), 55–61. Retrieved from http://www.thebioscan.in/Journals_PDF/10110 PUJAITAGHOSH.pdf

Hao, B., Li, W., Linchun, M., Li, Y., Rui, Z., Mingxia, T., & Weikai, B. (2006). A study of conservation genetics in Cupressus chengiana, an endangered endemic of China, using ISSR markers. Biochemical Genetics, 44(1-2), 29-43. https://doi.org/10.1007/s10528-006-9011-8

Heikal, A. H., Badawy, O. M., & Hafez, A. M. (2008). Genetic relationships among some Stevia (Stevia rebaudiana Bertoni) accessions based on ISSR analysis. Research Journal of Cell and Molecular Biology, 2(1), 1–5. Retrieved from http://arnmsmb.com/old/rjcmb/rjcmb/2008/1-5.pdf

Herison, C., Sutjahjo, S. H., Sulastrini, I., Rustikawati, & Marwiyah, S. (2018). Genetic diversity analysis in 27 tomato accessions using morphological and molecular markers. AGRIVITA Journal of Agricultural Science, 40(1), 36–44. https://doi.org/10.17503/agrivita.v40i1.726

Ibrahim, H. (1999). Kaempferia galanga L. In L. S. de Padua, N. Bunyapraphatsara, & R. H. M. J. Lemmons (Eds.), Plant resources of South-East Asia No. 12: Medicinal and poisonous plants 1 (p. 334). Leiden, The Netherlands: Backhuys Publishers. Retrieved from https://prota4u.org/prosea/view.aspx?id=329

Ismail, N. A., Rafii, M. Y., Mahmud, T. M. M., Hanafi, M. M., & Miah, G. (2019). Genetic diversity of torch ginger (Etlingera elatior) germplasm revealed by ISSR and SSR markers. BioMed Research International, 2019(5904804), 1–14. https://doi.org/10.1155/2019/5904804

Jianming, G., Shougong, Z., Liwang, Q., Yong, Z., Chunguo, W., & Wenqin, S. (2006). ISSR and AFLP identification and genetic relationships of Chinese elite accessions from the genus Populus. Annals of Forest Science, 63(5), 499-506. https://doi.org/10.1051/forest:2006031

Kumar, A. (2014). Chemical composition of essential oil isolated from the rhizomes of Kaempferia galanga L. International Journal of Pharma and Bio Sciences, 5(1), 225–231. Retrieved from https://ijpbs.net/abstract.php?article=Mjk5MA==

Moulin, M. M., Rodrigues, R., Gonçalves, L. S. A., Sudré, C. P., & Pereira, M. G. (2012). A comparison of RAPD and ISSR markers reveals genetic diversity among sweet potato landraces (Ipomoea batatas (L.) Lam.). Acta Scientiarum. Agronomy, 34(2), 139–147. https://doi.org/10.4025/actasciagron.v34i2.12616

Nei, M., & Li, W. H. (1979). Mathematical model for studying genetic variation in terms of restriction endonucleases. Proceedings of the National Academy of Sciences of the United States of America, 76(10), 5269–5273. https://doi.org/10.1073/pnas.76.10.5269

Pandotra, P., Gupta, A. P., Husain, M. K., Gandhiram, & Gupta, S. (2013). Evaluation of genetic diversity and chemical profile of ginger cultivars in northwestern Himalayas. Biochemical Systematics and Ecology, 48, 281–287. https://doi.org/10.1016/j.bse.2013.01.004

Pharmawati, M., & Candra, I. P. (2015). Genetic diversity of patchouli cultivated in Bali as detected using ISSR and RAPD markers. Biodiversitas, 16(2), 132–138. https://doi.org/10.13057/biodiv/d160205

Prashanth, N., Yugander, A., & Bhavani, N. L. (2015). DNA isolation and PCR amplification of turmeric varieties from Telangana State. International Journal of Current Microbiology and Applied Sciences, 4(5), 485–490. Retrieved from https://www.ijcmas.com/vol-4-5/N. Prashanth, et al.pdf

Preetha, T. S., Hemanthakumar, A. S., & Krishnan, P. N. (2016). A comprehensive review of Kaempferia galanga L. (Zingiberaceae): A high sought medicinal plant in Tropical Asia. Journal of Medicinal Plants Studies, 4(3), 270–276. Retrieved from http://www.plantsjournal.com/archives/2016/vol4issue3/PartD/4-3-8-414.pdf

Rajasekharan, P., Kareem, V. A., Ravish, B., & Mini, S. (2016). Analysis of genetic diversity in Alpinia galanga using ISSR markers. Indian Journal of Plant Genetic Resources, 29(2), 194–198. https://doi.org/10.5958/0976-1926.2016.00028.0

Rao V, N., & Kaladhar, D. (2014). Antioxidant and antimicrobial activities of rhizome extracts of Kaempferia galanga. World Journal of Pharmacy and Pharmaceutical Sciences, 3(5), 1180–1189. Retrieved from https://www.researchgate.net/publication/262817682_ANTIOXIDANT_AND_ANTIMICROBIAL_ACTIVITIES_OF_RHIZOME_EXTRACTS_OF_Kaempferia_galanga

Rohlf, F. J. (1998). NTSYS-p.c. numerical taxonomy and multivariate analysis system (Version 2.0). Setauket, NY: Exeter Software Publishers Ltd.

Saha, K., Sinha, R. K., Basak, S., & Sinha, S. (2016). ISSR fingerprinting to ascertain the genetic relationship of Curcuma sp. of Tripura. American Journal of Plant Sciences, 7(2), 259–266. https://doi.org/10.4236/ajps.2016.72025

Shafie, S. B., Zain Hasan, S. M., Zain, A. M., & Shah, R. M. (2011). RAPD and ISSR markers for comparative analysis of genetic diversity in wormwood capillary (Artemisia capillaris) from Negeri Sembilan, Malaysia. Journal of Medicinal Plant Research, 5(18), 4426–4437. Retrieved from http://www.academicjournals.org/app/webroot/article/article1380724457_Shafie etal.pdf

Shirin, F., Kumar, S., & Mishra, Y. (2000). In vitro plantlet production system for Kaempferia galanga, a rare Indian medicinal herb. Plant Cell, Tissue and Organ Culture, 63(3), 193–197. https://doi.org/10.1023/A:1010635920518

Singh, S., Panda, M. K., & Nayak, S. (2012). Evaluation of genetic diversity in turmeric (Curcuma longa L.) using RAPD and ISSR markers. Industrial Crops and Products, 37(1), 284–291. https://doi.org/10.1016/j.indcrop.2011.12.022

Taheri, S., Abdullah, T. L., Abdullah, N. A., & Ahmad, Z. (2012). Genetic relationships among five varieties of Curcuma alismatifolia (Zingiberaceae) based on ISSR markers. Genetics and Molecular Research, 11(3), 3069–3076. https://doi.org/10.4238/2012.August.31.4

Theanphong, O., Jenjittikul, T., Mingvanish, W., & Rungsihirunrat, K. (2018). Phylogenetic relationships of Kaempferia plants based on inter-simple sequence repeat fingerprints. Songklanakarin Journal of Science and Technology, 40(3), 617–622. https://doi.org/10.14456/sjst-psu.2018.81

Umar, M. I., Zaini Bin Asmawi, M., Sadikun, A., Altaf, R., & Iqbal, M. A. (2011). Phytochemistry and medicinal properties of Kaempferia galanga L. (Zingiberaceae) extracts. African Journal of Pharmacy and Pharmacology, 5(14), 1638–1647. https://doi.org/10.5897/AJPP11.388

Wahyono, S. (2013). Laporan Nasional: Eksplorasi pengetahuan lokal etnomedisin dan tumbuhan obat di Indonesia berbasis komunitas. Riset Tumbuhan Obat dan Jamu (RISTOJA) 2012. Jakarta, ID: Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan RI. Retrieved from http://labdata.litbang.depkes.go.id/images/download/laporan/RIKHUS/2012/LaporanNasional_Ristoja2012.pdf

Zheng, W. H., Zhuo, Y., Liang, L., Ding, W. Y., Liang, L. Y., & Wang, X. F. (2015). Conservation and population genetic diversity of Curcuma wenyujin (Zingiberaceae), a multifunctional medicinal herb. Genetics and Molecular Research, 14(3), 10422–10432. https://doi.org/10.4238/2015.September.8.3




DOI: http://doi.org/10.17503/agrivita.v42i1.1850

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.