Developing Blast Disease Resistance of Jasmine Rice by Phenotypic-Genotypic Simultaneous Selection

Thanakorn Wangsawang, Tanee Sreewongchai, Prapa Sripichitt, Fisseha Worede

Abstract


Breeding for resistant varieties of rice is known to be the most preferable way of controlling blast disease (Pyricularia oryzae). Identification and introduction of resistance genes into elite rice lines has become possible by the use of molecular markers. KD2-1 line is an isogenic line of KDML105 carrying four resistance genes on chromosome 2, 3, 8 and 12 from IR64 variety. The objective of this research was to transfer blast disease resistant genes from KD2-1 line into RD15 variety by using phenotypic and genotypic selections by the aid of markers. In this study, the four resistance genes were transferred from KD2-1 rice line into a blast susceptible rice variety, RD15. The study resulted in the breeding of four elite rice lines with four resistance genes by phenotypic and foreground selection. The genome-wide SSR marker analysis of the lines showed more than 86.5% background genome recovery of RD15. Pathogenicity assays of the four selected lines exhibited a resistant reaction to all 13 isolates, with agronomic and yield performance, and cooking and eating quality characteristics similar to that of RD15. The phenotypic-genotypic (foreground and background) simultaneous selection strategy is very useful to introduce multiple resistance genes in rice as it is a fast and economical way for identification of anticipated recombinant lines with desired genes.

Keywords


Genome recovery; Jasmine rice; Marker-assisted selection; Pyricularia oryzae

Full Text:

PDF

References


Abedi, F., Babaeiyan, N., & Moumeni, A. (2012). Performance of different rice genotypes against blast pathogen through linked molecular markers. Journal of Crop Science and Biotechnology, 15(2), 79–84. crossref

Hasan, M. M., Rafii, M. Y., Ismail, M. R., Mahmood, M., Rahim, H. A., Alam, M. A., … Latif, M. A. (2015). Marker-assisted backcrossing: A useful method for rice improvement. Biotechnology and Biotechnological Equipment, 29(2), 237–254. crossref

Hayashi, K., Yoshida, H., & Ashikawa, I. (2006). Development of PCR-based allele-specific and InDel marker sets for nine rice blast resistance genes. Theoretical and Applied Genetics, 113(2), 251–260. crossref

Jayawardana, W.A.D., Jayasekera, G.A.U., Wijesundera, R.L.C.,& Dissanayake, D.M.N. (2013). The phenotypic screening of rice varieties for blast resistance towards developing DNA markers linked to resistance genes. Paper presented at Proceedings of 69th Annual Sessions of Sri Lanka Association for Advancement of Science, Part1. Abstract 939/D, 69. Colombo, LK: SLAAS.

Joshi, B. K., Bimb, H. P., Parajuli, G., & Chaudhary, B. (2009). Molecular tagging, allele mining and marker aided breeding for blast resistance in rice. BSN EBulletin, 1, 1–23. Retrieved from website

Mackill, D. J. (1992). Inheritance of blast resistance in near-isogenic lines of rice. Phytopathology, 82(7), 746. crossref

NBACFS. (2003). Thai agricultural standard: Thai hom mali rice. Bangkok, TH: National Bureau of Agricultural Commodity and Food Standards, Ministry of Agriculture and Cooperatives. Retrieved from PDF

Randhawa, H. S., Mutti, J. S., Kidwell, K., Morris, C. F., Chen, X., & Gill, K. S. (2009). Rapid and targeted introgression of genes into popular wheat cultivars using marker-assisted background selection. PLoS ONE, 4(6), e5752. crossref

Roumen, E., Levy, M., & Notteghem, J. L. (1997). Characterisation of the European pathogen population of Magnaporthe grisea by DNA fingerprinting and pathotype analysis. European Journal of Plant Pathology, 103(4), 363–371. crossref

Sallaud, C., Meynard, D., van Boxtel, J., Gay, C., Bès, M., Brizard, J. P., ... Guiderdoni, E. (2003). Highly efficient production and characterization of T-DNA plants for rice (Oryza sativa L.) functional genomics. Theoretical and Applied Genetics, 106(8), 1396–1408. Retrieved from website

Sharma, T. R., Rai, A. K., Gupta, S. K., Vijayan, J., Devanna, B. N., & Ray, S. (2012). Rice blast management through host-plant resistance: Retrospect and prospects. Agricultural Research, 1(1), 37–52. crossref

Shin, M. S., Kim, K. Y., Park, H. S., &Ko, J. K. (2011). Breeding for resistance to bacterial blight in rice. Korean Journal of Breeding Science, 43, 251-261.

Sirithunya, P., Sriprakhon, S., Wongsaprom, C., Sreewongchai, T., Vanavichit, A.,& Toojinda, T. (2004). Discovery of broad spectrum blast resistance in rice. Paper presented at Proceedings of the 1st International Conference on Rice for the Future, Kasetsart University. Bangkok, TH: Kasetsart University.

Skamnioti, P., & Gurr, S. J. (2009). Against the grain: Safeguarding rice from rice blast disease. Trends in Biotechnology, 27(3), 141–150. crossref

Sreewongchai, T., Toojinda, T., Thanintorn, N., Kosawang, C., Vanavichit, A., Tharreau, D., & Sirithunya, P. (2010). Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolates by pyramiding multiple resistance QTLs. Plant Breeding, 129(2), 176–180. crossref

Sriboonjit, J. & Viboonpong, A. (2000). Evaluation of neck blast disease affected to KDML rice production by stochastic frontier method. Chiang Mai University Journal of Economics, 3, 39-52.

Srividhya, A., Vemireddy, L. R., Sridhar, S., Jayaprada, M., Ramanarao, P. V, Hariprasad, A. S., … Siddiq, E. (2011). Molecular mapping of QTLs for yield and its components under two water supply conditions in rice (Oryza sativa L.). Journal of Crop Science and Biotechnology, 14(1), 45–56. crossref

Suh, J. P., Jeung, J. U., Noh, T. H., Cho, Y. C., Park, S. H., Park, H. S., … Jena, K. K. (2013). Development of breeding lines with three pyramided resistance genes that confer broad-spectrum bacterial blight resistance and their molecular analysis in rice. Rice, 6(1), 1–11. crossref

Suh, J. P., Yang, S. J., Jeung, J. U., Pamplona, A., Kim, J. J., Lee, J. H., … Jena, K. K. (2011). Development of elite breeding lines conferring Bph18 gene-derived resistance to brown planthopper (BPH) by marker-assisted selection and genome-wide background analysis in japonica rice (Oryza sativa L.). Field Crops Research, 120(2), 215–222. crossref

Thippeswamy, S., Chandramohan, Y., Pravalika, K., Madhavilatha, B., & Samreen, Z. (2015). Tagging of seedling cold tolerance in rice (Oryza sativa L.) with molecular markers. International Journal of Plant, Animal and Environmental Sciences, 5(3), 144–150. Retrieved from PDF

Usman Ghazanfar, M., Wakil, W., & Sahi, S. (2009). Influence of various fungicides on the management of rice blast disease. Mycopath, 7(1), 29–34. Retrieved from PDF

Waiyalert, A., Sreewongchai, T., Chaisan, T., & Sripichitt, P. (2015). Mapping of blast disease resistance genes in BC2F6 population of the cross KDMl105 × IR64. Kasetsart Journal - Natural Science, 49(3), 327–334. Retrieved from website

Ye, G., & Smith, K. F. (2010). Marker-assisted gene pyramiding for cultivar development. Plant Breeding Reviews, 33, 219–256. crossref




DOI: http://doi.org/10.17503/agrivita.v40i2.1482

Copyright (c) 2018 UNIVERSITAS BRAWIJAYA

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.