BIOCONTROL FOR RHIZOCTONIA STEM ROT DISEASE BY USING COMBINATION OF SPECIFIC ENDOPHYTE IN PADDY TIDAL SWAMP

Ismed Setya Budi, Mariana Mariana

Abstract


The use of combination of specific endophytic in tidal swamps to control stem root disease as biological control agents has not been done. It is expected that this combination is able to continuously protect plants from pathogen interference. The research was carried out in type C tidal swamp in Banjar regency of South Kalimantan, from March to November 2011, temperature 29-32oC, and pH 4.0-5.5.  The method used was Split Plot design. Biocontrol preparation for both types of endophytic was applied in seeds in 7 days after planting (DAP). Observation on high intensity and plant diseases of planting stage on tidal swamps (taradak, ampak and lacak) was conducted. The results showed that there was a reduction of disease ranging from 58.70 to 87.29%. The application of combination of two biocontrol agents (T. viride PS-2.1 + P. fluorescent PS-4.8), (Fusarium non-pathogenic PS-1.5 + P. fluorescent PS-4.8) and (T. viride PS-2.1+ FNP PS-1.5) isolate gave the best inhibition result, reduced disease intensity, and increased plant height. The result of soil analysis before and after application of endophytic showed that there was an increase in soil fertility with the element addition of N, P, K and pH.

 

Keywords:  stem rot, endophytic combination, paddy, tidal swamp


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References


Almeida, F. B R., F.M. Cerqueira, R.N. Silva, C.J.´ Ulhoa, and A.L. Lima. 2007. Mycopara-sitism studies of Trichoderma harzianum strains against Rhizoctonia solani: evaluation of coiling and hydrolytic enzyme production. Biotechnol Lett 29: 1189–1193

Altomare, C., W.A. Norvell, T. Bjbrkmar and G.E. Harman. 1999. Solubilization of phosphates and micronutriens by the plantgrowth promoting and biocontrol fungus Trichoderma harzianum Rifai 1295-22. Applied Environ. Microbiol, 65: 2926-2933.

Benhamou,N., C. Garand and A Goulet. 2002. Ability of nonpathogenic Fusarium oxy-sporum Strain Fo47 to Induce resistence aggainst Pythium ultimum infection in cucumber. Applied Environ. Microbiol. 68: 4044-4060

Budi, I.S. and Mariana. 2012. Bioecology of non-pathogenic Fusarium specific location of Tidal Swamps. Proceeding Inter-national Seminar on University-Based Research for Wetland Development. p.157-167.

Budi, I.S. dan Mariana. 2009. Control on wilt disease on paddy in Tidal Swamp Area, South Kalimantan by using antagonist and botanic pesticide. Faculty of Agri-culture, Lambungmangkurat University, Banjarbaru

Cook, R.J. and K.F. Baker. 1983. The nature and practice of biological control of plant patho-gens. APS Press, St.Paul, MN, USA.

Dhingra,O.D. and J.B. Sinclair. 1995. Basic plant pathology methods. Second edition. CRC Press, Inc., Boca Raton. pp. 448

Duijff, B.J., D. Pouhair, C. Olivain, C. Alabouvette and P. Lemanceau. 1998. Implication of systemic induced resistance in the suppresion of fusarium wilt of tomato by Pseudomonas fluorescens WCS417r and by nonpathogenic Fusarium oxy-sporum Fo47. Eur. J. Palnt Pathol. 104: 903-910.

Fokkema, N.J., J.H. Bond and H.A. Fribourg. 1959. Methods for studying soil micro-flora plant disease relationships. Burgess Publ. Co. USA. pp. 247.

Fuchs, J.G., Y. Moenne-Loccoz and G. Defago. 1999. Nonpathogenic Fusarium oxy-sporum strain Fo47 to protect tomato against Fusarium wilt. Bio. Control, 4: 105-110 in tomato

Guetsky, R., D. Shtienberg, Y. Elad and A. Dinoor. 2001. Combining biocontrol Agents to reduce the variability of biological control. Phytopathol. 91: 621-627.

Hanson, L.E. and C.R. Howell. 2004. Elicitors of plant defense responses from biocontrol strains of Trichoderma virens. Phytopathol. 94:171-176.

Harman, G.E. 2006. Overview of mechanisms and uses of Trichoderma spp. Phytopatol. 96:190-194

Howell, C.R. and Stipanovic. 1995. Mechanisms in the control of Rhizoctonia solani induced cotton seedling disese by Gliocladium virens antibiosis. Phytopathol J. 85: 469-472

Jager G. and H. Velvis. 1988. Inactivation of sclerotia of Rhizoctonia solani on potato tubers by Verticillium biguttatum, a soil-borne mycoparasite. Netherlands Journal of Plant Pathology 94(5): 225-231

Kwok, O.C H., P.C. Gahy, H.A.J. Hoitink, and G.A. Kuter. 1987. Interactions between bacteria and Trichoderma hamatum in suppression of Rhizoctonia damping-off in bark compost media. Phytopathology 77:1206-1212.

Nel, B., C. Steinberg, N. Labuschagne and A. Vilioen. 2006. The potential of non-pathogenic Fusarium oxysporum and other biological control organisms for suppressing fusarium wilt of banana. Plant Pathol. J. 1(55): 217-223

Olivain C., C. Humbert, J. Nahalkova, J. Fatehi, F.L.Haridon and C. Alabouvette. 2006. Colonization of tomato root by pathogenic and nonpathogenic Fusarium oxysporum strains inoculated together and separately into the soil. Appl. Environ. Microbiol. 72(2): 1523-1531

Ozbay, N. and S. Newman. 2004. Biocontrol with Trichoderma spp with emphasis on T. harzianum. Pakistan Journal of Biological Science 7(4):478-484

Pal, K. K. and B. McSpadden Gardener, 2011. Biological Control of Plant Pathogens. The Plant Health Instructor DOI: 10.1094/PHI-A-2006-1117-02. https://www.apsnet.org/edcenter/advanced/topics/Pages/BiologicalControl.aspx

Sands, D.C. and A.D. Rovira. 1970. Isolation of fluorescent pseudomonads with a selective medium. Applied Microbiology 20 (3) : 513-514.

Thomashow, L.S. and D.M. Weller. 1996. Current concepts in the use of introduced bacteria for biological disease control: Mechanisms and antifungal metabolites. In: Plant-Microbe Interactions. Stacey, G. and N.T. Keen (Eds) New York: Chapman and Hill. Pp:236-271

Van Den Boogert, P.H.J.F. and J.W. Deacon. 1994. Biotrophic mycoparasitism by Verticillium biguttatum on Rhizoctonia solani. European Journal of Plant Patho-logy 100(2):137-156.

Weller, D. M. 1988. Biological control of soilborne plant pathogens in the hizosphere with bacteria. Annu. Rev. Phytopathol. 26: 379-407.

Xu, X-M. and M,J, Jeger. 2013. Theoretical mo-delling suggests that synergy may result from combined use of two biocontrol agents for controlling foliar pathogens under spatial heterogeneous conditions. Phytopathology. 103(8): 768-775

Yigit, F. and M. Dikilitas. 2007. Control of Fusarium wilt of tomato by combination of fluores-cent Pseudomonas, Non-pathogen Fu-sarium and Trichoderma harzianum T-22 in Greenhouse Conditions. Plant Pathol. Journal 6(2):159-163




DOI: http://doi.org/10.17503/agrivita.v35i3.125

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