Application of A New Bio-Formulation of Chaetomium cupreum For Biocontrol of Colletotrichum gloeosporioides Causing Coffee Anthracnose on Arabica Variety in Laos

Somlit Vilavong, Kasem Soytong


The anthracnose pathogen was isolated from coffee leave and bean symptoms. Morphological and molecular phylogenetic data confirmed the species as Colletotrichum gloeosporioides. The pathogenicity of the isolate was also confirmed by detached leaf method which inoculated the virulent isolate into coffee leaves. The crude extracts with hexane, ethyl acetate and methanol solvents from Chaetomium cupreum CC3003 resulted significantly inhibited C. gloeosporioides that the ED50 values of 13, 11 and 28 ppm, respectively. The bioactive substances of C. cupreum CC3003 expressed antifungal activity against C. gloeosporioides as can be seen in the abnormal appearance of spores. A powder bio-formulation of C. cupreum significantly resulted to reduce anthracnose disease of 54.77 %. The application of nano-rotiorinol, nano-trichotoxin and a spore suspension of C. cupreum reduced anthracnose incidence of 46.23, 42.71 and 18.59 %, respectively while the inoculated control had high anthracnose disease. The application of bio-formulation of C. cupreum in powder form, nano-rotiorinol, and nano-trichotoxin to reduce coffee anthracnose was reported for the first time in Lao PDR.


anthracnose; coffee; nano-elicitors; plant immunity

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Bailey, J. A., & Jeger, M. J. (1992). Colletotrichum: Biology, pathology and control (1st ed.). London, UK: CABI.

Clifford, M. N., & Helm, C. (1985). Coffee: Botany, biochemistry, and production of beans and beverage. London, UK: The AVI Publishing Company.

Dar, J., & Soytong, K. (2014). Construction and characterization of copolymer nanomaterials loaded with bioactive compounds from Chaetomium species. International Journal of Agricultural Technology, 10(4), 823-831. Retrieved from, Joselito and KasemSoytong - Agricultural Nanotechnology-edited.pdf

Ditta, A. (2012). How helpful is nanotechnology in agriculture? Advances in Natural Sciences: Nanoscience and Nanotechnology, 3(3), 33002. crossref

Heye, C. C., & Andrews, J. H. (1983). Antagonism of Athelia bombucina and Chaetomium globosum to the apple scab pathogen, Venturia inequalis. Phytopathology, 76, 650-654.

Kanokmedhakul, S., Kanokmedhakul, K., Nasomjai, P., Louangsysouphanh, S., Soytong, K., Isobe, M., .... Suksamrarn, A. (2006). Antifungal azaphilones from the fungus Chaetomium cupreum CC3003. Journal of Natural Products, 69(6), 891–895. crossref

Köhl, J., Molhoek, W. M. L., van der Plas, C. H., & Fokkema, N. J. (1995). Effect of Ulocladium atrum and other antagonists on sporulation of Botrytis cinerea on dead lily leaves exposed to field conditions. Phytopathology, 85, 393-401.

Li, M., Huang, Q., & Wu, Y. (2011). A novel chitosan-poly(lactide) copolymer and its submicron particles as imidacloprid carriers. Pest Management Science, 67(7), 831–836. crossref

Ministry of Agriculture and Forestry. (1997). Agriculture statistic year 1997. Laos: Department of Planning.

Neuvéglise, C., Brygoo, Y., Vercambre, B., & Riba, G. (1994). Comparative analysis of molecular and biological characteristics of strains of Beauveriabrongniartii isolated from insects. Mycological Research, 98(3), 322-328. crossref

Perlatti, B., de Souza Bergo, P. L., Fernandes da Silva, M. F. das G., Fernandes, J. B., & Forim, M. R. (2013). Polymeric nanoparticle-based insecticides: A controlled release purpose for agrochemicals. In S. Trdan (Ed.), Insecticides - Development of safer and more effective technologies (pp. 523–550). InTech. crossref

Ratanacherdchai, K., Wang, H. K., Lin, F. C., & Soytong, K. (2010). ISSR for comparison of cross-inoculation potential of Colletotrichum capsici causing chilli anthracnose. African Journal of Microbiology Research, 4(2), 076–083. Retrieved from website

Ridler, N. B. (1983). Labour force and land distributional effects of agricultural technology: A case study of coffee. World Development, 11(7), 593–599. crossref

Soutter, W. (2012). Nanotechnology in agriculture - Azono: The A-Z of nanotechnology. Retrieved from website

Soytong, K., Kanokmedhakul, S., Kukongviriyapa, V., & Isobe, M. (2001). Application of Chaetomium species (Ketomium) as a new broad spectrum biological fungicide for plant disease control: A review article. Fungal Diversity, 7, 1–15. Retrieved from PDF

Sutton, B. C. (1980). The coelomycetes: Fungi imperfecti with pycnidia, acervuli and stromata. Kew, UK: Commonwealth Mycological Institute.

Suwan, S., Isobe, M., Kanokmedhakul, S., Lourit, N., Kanokmedhakul, K., Soytong, K., & Koga, K. (2000). Elucidation of high micro-heterogeneity of an acidic–neutral trichotoxin mixture from Trichodermaharzianum by electrospray ionization quadrupole time-of-flight mass spectrometry. Journal of Mass Specrometry, 35(12), 1438–1451.<1438::AID-JMS80>3.0.CO;2-Q

Tveit, M., & Moore, M. B. (1954). Isolates of Chaetomium that protect oats from Helminthosporium victoriae. Phytophatology, 44, 686-689.



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