Production Enhancement Technology of Pegagan (Centella asiatica)

Noverita Sprinse Vinolina, Marline Nainggolan, Rolan Siregar


Centella asiatica has long been known as one of medicinal plants. Profound study is needed to be able to find out the responses of Pegagan to various treatments in order to increase centelloside content. It was also previously reported that the highest asiaticoside content was found at the lowland pegagan, Pantai Labu. Current study on planted lowland pegagan of Deli Serdang with 2.38% of asiaticoside content was aimed to know the response of the plants to phosphorus fertilizing, elicitorand harvesting time related to biomass production from leaves, petioles, roots and tendrils. Phosphorus fertilizer was applied in four levels: 0, 18, 36 and 54 kg P2O5.ha-1, which affected centelloside synthesis, and elicitor of methyl jasmonate was given at three levels: 0, 100, 200 μM, while harvesting time was set up at 56, 70 and 84 days after planting (DAP). Data showed that the best combination for biomass production was obtained when there was no elicitor and harvesting was done at 84 DAP. The best combination between methyl jasmonate hormone and harvesting period in both fresh weight of leaves and petioles as well as roots was obtained when there was no hormone  and harvesting time was 84 days old.


Centella asiatica; harvesting; methyl jasmonate; phosphorous; production

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Bermawie, N., Purwiyanti, S., & Mardiana. (2008). Keragaan sifat morfologi, hasil dan mutu plasma nutfah pegagan (Centella asiatica (L.) Urban.) [Variation in morphological characteristics, yield and quality of asiatic pennywort (Centella asiatica (L.) Urban) germplasm]. Buletin Penelitian Tanaman Rempah dan Obat, 19(1), 1-17. Retrieved from

Bidel, L. P. R., Chomicki, G., Bonini, F., Mondolot, L., Soulé, J., Coumans, M., … Jay-Allemand, C. (2015). Dynamics of flavonol accumulation in leaf tissues under different UV-B regimes in Centella asiatica (Apiaceae). Planta, 242(3), 545–559.

Bonfill, M., Mangas, S., Moyano, E., Cusido, R. M., & Palazón, J. (2011). Production of centellosides and phytosterols in cell suspension cultures of Centella asiatica. Plant Cell, Tissue and Organ Culture, 104(1), 61–67.

Chaisawang, P., A. Sirichoat, W. Chaijaroonkhanarak, W. Pannangrong, B. Sripanidkulchai, P. Wigmore, & J. Umka Welbat. (2017). Asiatic acid protects against cognitive deficits and reductions in cell proliferation and survival in the rat hippocampus caused by 5-fluorouracil chemotherapy. PLOS ONE htpps://

Erbilgin, N., Krokene, P., Christiansen, E., Zeneli, G., & Gershenzon, J. (2006). Exogenous application of methyl jasmonate elicits defenses in Norway spruce (Picea abies) and reduces host colonization by the bark beetle Ips typographus. Oecologia, 148(3), 426–436.

Ghulamahdi, M., Aziz, S. A., Bermawie, N., & Hernani. (2007). Evaluasi karakter morfologi, fisiologi dan genetik pegagan mendukung standarisasi mutu pegagan [Evaluation of pegagan based on its morphological, physiological and gentics appearances to support quality standardization of pegagan]. Executive Resume: 2007 Research Results (pp. 76-77). Cooperation Partnership between Agricultural Research And Colleges. Retrieved from

Kim, O. T., Kim, M. Y., Hong, M. H., Ahn, J. C., & Hwang, B. (2004). Stimulation of asiaticoside accumulation in the whole plant cultures of Centella asiatica (L.) Urban by elicitors. Plant Cell Reports, 23(5), 339–344.

Lambert, E., Faizal, A., & Geelen, D. (2011). Modulation of triterpene saponin production: In vitro cultures, elicitation, and metabolic engineering. Applied Biochemistry and Biotechnology, 164(2), 220–237.

Mangas, S., Bonfill, M., Osuna, L., Moyano, E., Tortoriello, J., Cusido, R. M., … Palazón, J. (2006). The effect of methyl jasmonate on triterpene and sterol metabolisms of Centella asiatica, Ruscus aculeatus and Galphimia glauca cultured plants. Phytochemistry, 67(18), 2041–2049.

Mangas, S., Moyano, E., Hernandez-Vazquez, L., & Bonfill, M. (2009). 4. Centella asiatica (L) Urban: An updated approach. In J. Palazón & R. M. Cusidó (Eds.). Plant Secondary Terpenoids, Research Signpost, 37/661(2), 55-74. Retrieved from ttps://

Mangas, S., Moyano, E., Osuna, L., Cusido, R. M., Bonfill, M., & Palazón, J. (2008). Triterpenoid saponin content and the expression level of some related genes in calli of Centella asiatica. Biotechnology Letters, 30(10), 1853–1859.

Matsuda, H., Morikawa, T., Ueda, H., & Yoshikawa, M. (2001). Medicinal foodstuffs. XXVII. Saponin constituents of gotu kola (2): Structures of new ursane- and oleanane-type triterpene oligoglycosides, centellasaponins B, C, and D, from Centella asiatica cultivated in Sri Lanka. Chemical and Pharmaceutical Bulletin, 49(10), 1368-1371.

Reinbothe, C., Springer, A., Samol, I., & Reinbothe, S. (2009). Plant oxylipins: Role of jasmonic acid during programmed cell death, defence and leaf senescence. FEBS Journal, 276(17), 4666-4681.

Salisbury, F. B., & Ross, C.W. (1995). Fisiologi tumbuhan, Jilid 1 [Plant physiology, 1st ed.]. Bandung: Penerbit ITB.

Sirichoat, A., Chaijaroonkhanarak, W., Prachaney, P., Pannangrong, W., Leksomboon, R., Chaichun, A., … Welbat, J. U. (2015). Effects of Asiatic acid on spatial working memory and cell proliferation in the adult rat hippocampus. Nutrients, 7(10), 8413–8423.

Taiz, L.,& Zeiger, E. (2006).Plant physiology(4th ed.). Sunderland, MA: Sinauer Associates.

Vinolina, N. S., Napitupulu, J. A., Siregar, L. A. M., Nainggolan, M., & Singh, N. (2013). Centelloside content of pegagan (Centella asiatica). Indian Journal of Applied Research, 3(12), 1-2. Retrieved from

Yendo, A. C. A., de Costa, F., Gosmann, G., & Fett-Neto, A. G. (2010). Production of plant bioactive Triterpenoid saponins: Elicitation strategies and target genes to improve yields. Molecular Biotechnology, 46(1), 94-104.

Zeneli, G., Krokene, P., Christiansen, E., Krekling, T., & Gershenzon, J. (2006). Methyl jasmonate treatment of mature Norway spruce (Picea abies) trees increases the accumulation of terpenoid resin components and protects against infection by Ceratocystis polonica, a bark beetle-associated fungus. Tree Physiology, 26(8), 977–988.

Zhao, C., Xu, T., Liang, Y., Zhao, S., Ren, L., Wang, Q., & Dou, B. (2015). Functional analysis of β-amyrin synthase gene in ginsenoside biosynthesis by RNA interference. Plant Cell Reports, 34(8), 1307–1315.


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