Shoot and Root Growth in Common Bean (Phaseolus vulgaris L.) Exposed to Gradual Drought Stress

Laily Ilman Widuri, Benyamin Lakitan, Erizal Sodikin, Mery Hasmeda, Mei Meihana, Kartika Kartika, Erna Siaga


Drought condition during the dry season is a major constraint for intensifying agricultural activities at riparian wetlands in Indonesia, particularly for annual vegetables, including common bean (Phaseolus vulgaris L.). Besides inhibiting growth and reducing yield, drought also causes alteration of the shoot and root growth and development. The objective of this study was to evaluate responses of common bean to three durations of drought stress and the bean ability to recover after termination of the stress treatments. Gradual drought stress treatments were imposed by withholding all water sources to the treated plants. Three durations of drought stress imposed were 4, 8, and 12 days. The ability of the stress-treated plants to recover was evaluated at 7 days after termination of each treatment. The result of this study revealed that common bean was able to tolerate and recover from gradual water deficit for up to 8 days; however, prolonged water deficit for 12 days inhibited the growth of above-ground organs in common bean. Despite root regrowth during the recovery period, plants previously treated with 12 days of drought were unable to recover but those treated with shorter drought stress period were able to recover.


Drought stress; Growth analysis; Riparian wetland; Stress recovery; Water deficit

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Ahmad, N., Malagoli, M., Wirtz, M., & Hell, R. (2016). Drought stress in maize causes differential acclimation responses of glutathione and sulfur metabolism in leaves and roots. BMC Plant Biology, 16, 247. crossref

Albert, R., Acharya, B. R., Jeon, B. W., Zañudo, J. G. T., Zhu, M., Osman, K., & Assmann, S. M. (2017). A new discrete dynamic model of ABA-induced stomatal closure predicts key feedback loops. PLoS Biology, 15(9), e2003451. crossref

Ammar, M. H., Anwar, F., El-Harty, E. H., Migdadi, H. M., Abdel-Khalik, S. M., Al-Faifi, S. A., … Alghamdi, S. S. (2015). Physiological and yield responses of faba bean (Vicia faba L.) to drought stress in managed and open field environments. Journal of Agronomy and Crop Science, 201(4), 280–287. crossref

Avramova, V., Nagel, K. A., Abdelgawad, H., Bustos, D., Duplessis, M., Fiorani, F., & Beemster, G. T. S. (2016). Screening for drought tolerance of maize hybrids by multi-scale analysis of root and shoot traits at the seedling stage. Journal of Experimental Botany, 67(8), 2453–2466. crossref

Bahadur, A., Chatterjee, A., Kumar, R., Singh, M., & Naik, P. (2011). Physiological and biochemical basis of drought tolerance in vegetables. International Journal of Vegetable Science, 38(1), 1–16. Retrieved from PDF

Blum, A. (2017). Osmotic adjustment is a prime drought stress adaptive engine in support of plant production. Plant Cell and Environment, 40(1), 4–10. crossref

Borgo, L., Marur, C. J., & Vieira, L. G. E. (2015). Effects of high proline accumulation on chloroplast and mitochondrial ultrastructure and on osmotic adjustment in tobacco plants. Acta Scientiarum. Agronomy, 37(2), 191–199. crossref

Chitwood, D. H., & Sinha, N. R. (2016). Evolutionary and environmental forces sculpting leaf development. Current Biology, 26(7), R297–R306. crossref

Díaz-López, L., Gimeno, V., Simón, I., Martínez, V., Rodríguez-Ortega, W. M., & García-Sánchez, F. (2012). Jatropha curcas seedlings show a water conservation strategy under drought conditions based on decreasing leaf growth and stomatal conductance. Agricultural Water Management, 105, 48–56. crossref

Eisenach, C., Baetz, U., Huck, N. V., Zhang, J., De Angeli, A., Beckers, G., & Martinoia, E. (2017). ABA-induced stomatal closure involves ALMT4, a phosphorylation-dependent vacuolar anion channel of arabidopsis. Plant Cell, 29(10), 2552–2569. crossref

Erice, G., Louahlia, S., Irigoyen, J. J., Sanchez-Diaz, M., & Avice, J. C. (2010). Biomass partitioning, morphology and water status of four alfalfa genotypes submitted to progressive drought and subsequent recovery. Journal of Plant Physiology, 167(2), 114–120. crossref

Farooq, M., Wahid, A., Kobayashi, N., Fujita, D., & Basra, S. M. A. (2009). Plant drought stress: Effects, mechanisms and management. Agronomy for Sustainable Development, 29(1), 185–212. crossref

Feller, U. (2016). Drought stress and carbon assimilation in a warming climate: Reversible and irreversible impacts. Journal of Plant Physiology, 203, 84–94. crossref

Franco, J., Banon, S., Vicente, M. J., Miralles, J., & Martinez-Sanchez, J. (2011). Root development in horticultural plants grown under abiotic stress conditions – a review. Journal of Horticultural Science & Biotechnology, 86(6), 543–556. crossref

García-Castro, A., Volder, A., Restrepo-Diaz, H., Starman, T. W., & Lombardini, L. (2017). Evaluation of different drought stress regimens on growth, leaf gas exchange properties, and carboxylation activity in purple passionflower plants. Journal of the American Society for Horticultural Science, 142(1), 57–64. crossref

Garssen, A. G., Verhoeven, J. T. A., & Soons, M. B. (2014). Effects of climate-induced increases in summer drought on riparian plant species: A meta-analysis. Freshwater Biology, 59(5), 1052–1063. crossref

Golldack, D., Li, C., Mohan, H., & Probst, N. (2014). Tolerance to drought and salt stress in plants: Unraveling the signaling networks. Frontiers in Plant Science, 5, 151. crossref

Kavas, M., Baloǧlu, M. C., Akça, O., Köse, F. S., & Gökçay, D. (2013). Effect of drought stress on oxidative damage and antioxidant enzyme activity in melon seedlings. Turkish Journal of Biology, 37, 491–498. crossref

Lakitan, B., Widuri, L. I., & Meihana, M. (2017). Simplifying procedure for a non-destructive, inexpensive, yet accurate trifoliate leaf area estimation in snap bean (Phaseolus vulgaris). Journal of Applied Horticulture, 19(1), 15–21. Retrieved from website

Lanna, A. C., Mitsuzono, S. T., Terra, T. G. R., Vianello, R. P., & de Figueiredo Carvalho, M. A. (2016). Physiological characterization of common bean (Phaseolus vulgaris L.) genotypes, water- stress induced with contrasting response towards drought. Australian Journal of Crop Science, 10(1), 1–6. Retrieved from PDF

Li, W., Herrera-Estrella, L., & Tran, L. S. P. (2016). The yin-yang of cytokinin homeostasis and drought acclimation/adaptation. Trends in Plant Science, 21(7), 548–550. crossref

Mathobo, R., Marais, D., & Steyn, J. M. (2017). The effect of drought stress on yield, leaf gaseous exchange and chlorophyll fluorescence of dry beans (Phaseolus vulgaris L.). Agricultural Water Management, 180(Part A), 118–125. crossref

Nassar, R. M. A., Ahmed, Y. M., & Boghdady, M. S. (2010). Botanical studies on Phaseolus vulgaris L. I-morphology of vegetative and reproductive growth. International Journal of Botany, 6(3), 323–333. crossref

Nazar, R., Umar, S., Khan, N. A., & Sareer, O. (2015). Salicylic acid supplementation improves photosynthesis and growth in mustard through changes in proline accumulation and ethylene formation under drought stress. South African Journal of Botany, 98, 84–94. crossref

Neves, D. M., Almeida, L. A. D. H., Santana-Vieira, D. D. S., Freschi, L., Ferreira, C. F., Soares Filho, W. D. S., … Gesteira, A. D. S. (2017). Recurrent water deficit causes epigenetic and hormonal changes in citrus plants. Scientific Reports, 7(13684), 1–11. crossref

Olawuyi, O. J., Bello, O. B., Ntube, C. V., & Akanmu, A. O. (2015). Progress from selection of some maize cultivars’ response to drought in the derived Savanna of Nigeria. AGRIVITA Journal of Agricultural Science, 37(1), 8–17. crossref

Pozo, M. J., López‐Ráez, J. A., Azcón‐Aguilar, C., & García‐Garrido, J. M. (2015). Phytohormones as integrators of environmental signals in the regulation of mycorrhizal symbioses. New Phytologist, 205(4), 1431–1436. crossref

Riboldi, L. B., Oliveira, R. F., & Angelocci, L. R. (2016). Leaf turgor pressure in maize plants under water stress. Australian Journal of Crop Science, 10(6), 878–886. crossref

Rivas, R., Falcão, H. M., Ribeiro, R. V., Machado, E. C., Pimentel, C., & Santos, M. G. (2016). Drought tolerance in cowpea species is driven by less sensitivity of leaf gas exchange to water deficit and rapid recovery of photosynthesis after rehydration. South African Journal of Botany, 103, 101–107. crossref

Rowe, J. H., Topping, J. F., Liu, J., & Lindsey, K. (2016). Abscisic acid regulates root growth under osmotic stress conditions via an interacting hormonal network with cytokinin, ethylene and auxin. New Phytologist, 211(1), 225–239. crossref

Sankar, B., Gopinathan, P., Karthishwaran, K., & Somasundaram, R. (2014). Variation in growth of peanut plants under drought stress condition and in combination with paclobutrazol and abscisic acid. Current Botany, 5, 14–21. Retrieved from website

Sapeta, H., Costa, J. M., Lourenço, T., Maroco, J., van der Linde, P., & Oliveira, M. M. (2013). Drought stress response in Jatropha curcas: Growth and physiology. Environmental and Experimental Botany, 85, 76–84. crossref

Sudrajat, D. J., Siregar, I. Z., Khumaida, N., Siregar, U. J., & Mansur, I. (2015). Adaptability of white jabon (Anthocephalus cadamba MIQ.) seedling from 12 populations to drought and waterlogging. AGRIVITA Journal of Agricultural Science, 37(2), 130–143. crossref

Taiwo, O. J. (2013). Farmers’ choice of wetland agriculture: Checking wetland loss and degradation in Lagos State, Nigeria. GeoJournal, 78(1), 103–115. crossref

Tapia, G., Méndez, J., & Inostroza, L. (2016). Different combinations of morpho-physiological traits are responsible for tolerance to drought in wild tomatoes Solanum chilense and Solanum peruvianum. Plant Biology, 18(3), 406–416. crossref

Tombesi, S., Nardini, A., Frioni, T., Soccolini, M., Zadra, C., Farinelli, D., … Palliotti, A. (2015). Stomatal closure is induced by hydraulic signals and maintained by ABA in drought-stressed grapevine. Scientific Reports, 5(12449), 1–12. crossref

Widuri, L. I., Lakitan, B., Hasmeda, M., Sodikin, E., Wijaya, A., Meihana, M., … Siaga, E. (2017). Relative leaf expansion rate and other leaf-related indicators for detection of drought stress in chili pepper (Capsicum annuum L.). Australian Journal of Crop Science, 11(12), 1617–1625. crossref

Xiong, D., Yu, T., Ling, X., Fahad, S., Peng, S., Li, Y., & Huang, J. (2015). Sufficient leaf transpiration and nonstructural carbohydrates are beneficial for high-temperature tolerance in three rice (Oryza sativa) cultivars and two nitrogen treatments. Functional Plant Biology, 42(4), 347–356. crossref

Zhao, R., Guo, W., Bi, N., Guo, J., Wang, L., Zhao, J., & Zhang, J. (2015). Arbuscular mycorrhizal fungi affect the growth, nutrient uptake and water status of maize (Zea mays L.) grown in two types of coal mine spoils under drought stress. Applied Soil Ecology, 88, 41–49. crossref


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