Compatibility Test of Four Trichoderma spp. Isolates on Several Synthetic Pesticides

Loekas Soesanto, Endang Mugiastuti, Ruth Feti Rahayuniati, Abdul Manan, Ratna Stia Dewi


This research aimed to study the compatibility of some Trichoderma spp. isolates on some synthetic chemical pesticides carried out at the Laboratory of Plant Protection, Faculty of Agriculture, Jenderal Soedirman University from April up to July 2014. Trichoderma isolates were derived from rhizosphere exploration on ginger, banana, pineapple and shallot. The synthetic pesticides used were mancozeb and propineb (fungicides), oxytetracycline and streptomycin sulfate (agrimycin, bactericides), carbofuran (nematicide), and deltamethrin and prefenophos (insecticides: synthetic pyrethroids and chiral organophosphates, respectively). The compatibility test used food poisoning method in a completely randomized design with three replicates. Variables observed were discolouration, sporulation, colony diameter, conidia density, and fungal growth at pesticides treatment. The data were analyzed by F test at 5 % significant level and continued by Duncan Multiple Range Test (DMRT) when there was a significant difference. The result of the research showed that the most significant decreasing of Trichoderma spp. was found on mancozeb for shallot, ginger, and banana isolates, and propineb for pineapple isolate, respectively, 89.4, 97.7, 93.3, and 95.2 %. This result was in line with colour, sporulation, and inhibition level observation.


Compatibility; Synthetic pesticides; Trichoderma spp.

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Aktar, M. W., Sengupta, D., & Chowdhury, A. (2009). Impact of pesticides use in agriculture: Their benefits and hazards. Interdisciplinary Toxicology, 2(1), 1–12.

Bagwan, N. B. (2010). Evaluation of Trichoderma compatibility with fungicides, pesticides, organic cakes and botanicals for integerated management of soil borne diseases of soybean [Glycine max (L.) Merril]. International Journal of Plant Protection, 3(2), 206–209. Retrieved from 16b3822e866d7d80fd520b3176781785ae95.pdf

Bailey, B. A., Bae, H., Strem, M. D., Crozier, J., Thomas, S. E., Samuels, G. J., … Holmes, K. A. (2008). Antibiosis, mycoparasitism, and colonization success for endophytic Trichoderma isolates with biological control potential in Theobroma cacao. Biological Control, 46(1), 24–35.

Barakat, F. M., Abada, K. A., Abou-Zeid, N. M., & El-Gammal, Y. H. E. (2014). Effect of volatile and non-volatile compounds of Trichoderma spp. on Botrytis fabae the causative agent of faba bean chocolate spot. American Journal of Life Sciences, 2(6–2), 11.

Benhamou, N., & Chet, I. (1997). Cellular and molecular mechanisms involved in the interaction between Trichoderma harzianum and Pythium ultimum. Applied and Environmental Microbiology, 63(5), 2095–2099. Retrieved from

Benítez, T., Rincón, A. M., Limón, M. C., & Codón, A. C. (2004). Biocontrol mechanisms of Trichoderma strains. International Microbiology, 7, 249–260. Retrieved from

Bhatnagar, H. (1995). Integrated use of biocontrol agents with fungicides to control wilt incidence in pigeon-pea. World Journal of Microbiology & Biotechnology, 11(5), 564–566.

Černohlávková, J., Jarkovský, J., & Hofman, J. (2009). Effects of fungicides mancozeb and dinocap on carbon and nitrogen mineralization in soils. Ecotoxicology and Environmental Safety, 72(1), 80–85.

Chowdhury, A., Pradhan, S., Saha, M., & Sanyal, N. (2008). Impact of pesticides on soil microbiological parameters and possible bioremediation strategies. Indian Journal of Microbiology, 48(1), 114–127.

Cooper, J., & Dobson, H. (2007). The benefits of pesticides to mankind and the environment. Crop Protection, 26(9), 1337–1348.

Cycoń, M., Piotrowska-Seget, Z., & Kozdrój, J. (2010). Responses of indigenous microorganisms to a fungicidal mixture of mancozeb and dimethomorph added to sandy soils. International Biodeterioration and Biodegradation, 64(4), 316–323.

Day, K. E., & Maguire, R. J. (1990). Acute toxicity of isomers of the pyrethroid insecticide deltamethrin and its major degradation products to Daphnia magna. Environmental Toxicology and Chemistry, 9, 1297–1300.

Direktorat Pupuk dan Pestisida. (2016). Pestisida pertanian dan kehutanan tahun 2016 [Pesticides of agriculture and forestry 2016]. Jakarta: Ditjen PSP. Retrieved from Pertanian dan Kehutanan Tahun 2016.pdf

Dwimartina, F., Arwiyanto, T., & Joko, T. (2017). Potential of endophytic and rhizobacteria as an effective biocontrol for Ralstonia syzygii subsp. syzygii. Asian Journal of Plant Pathology, 11, 191–198.

El Khoury, W., & Makkouk, K. (2010). Integrated plant disease management in developing countries. Journal of Plant Pathology, 92(4, supplement), 35–42. Retrieved from

Elad, Y., Barak, R., & Chet, I. (1984). Parasitism of sclerotia of Sclerotium rolfsii by Trichoderma harzianum. Soil Biology and Biochemistry, 16(4), 381–386.

Erper, I., Turkkan, M., Atanasova, L., Druzhinina, I. S., Karaca, G. H., & Cebi-Kilicoglu, M. (2013). Integrated assessment of the mycoparasitic and phytostimulating properties of Trichoderma strains against Rhizoctonia solani. Bulgarian Journal of Agricultural Science, 19(4), 742–748. Retrieved from

Gómez, I., Chet, I., & Herrera-Estrella, A. (1997). Genetic diversity and vegetative compatibility among Trichoderma harzianum isolates. Molecular and General Genetics, 256(2), 127–135.

Gowdar, S. B., Babu, H. N. R., Nargund, V. B., & Krishnappa, M. (2006). Compatibility of fungicides with Trichoderma harzianum. Agricultural Science Digest, 26(4), 279–281. Retrieved from 50fa787ee5438f3a2600007e&assetKey= AS%3A271751534055425%4014418020 22498

Haggag, W. M., & Abo-Sedera, S. A. (2005). Characteristics of three Trichoderma species in peanut haulms compost involved in biocontrol of cumin wilt disease. International Journal of Agriculture and Biology, 7(2), 222–229. Retrieved from

Harman, G. E. (2006). Overview of mechanisms and uses of Trichoderma spp. Phytopathology, 96(2), 190–194.

Haryono, J., Prihatiningsih, N., Wardhana, R. A., & Soesanto, L. (2009). Pengaruh pemasteuran tanah tunggal atau digabung agensia hayati terhadap pengelolaan penyakit busuk hati di pembibitan pisang [The effect of soil pasteurization alone or in combination with biological agents on heart rot disease management of banana seed]. Akta Agrosia, 12(1), 21–28. Retrieved from No.1.pdf

Henis, Y., Adams, P. B., Lewis, J. A., & Papavizas, G. C. (1983). Penetration of sclerotia of Sclerotium rolfsii by Trichoderma spp. Phytopathology, 73, 1043–1046. Retrieved from

Howell, C. R. (2003). Mechanisms employed by Trichoderma species in the biological control of plant diseases: The history and evolution of current concepts. Plant Disease, 87(1), 4–10.

Jegathambigai, V., Wilson Wijeratnam, R. S., & Wijesundera, R. L. C. (2010). Effect of Trichoderma sp. on Sclerotium rolfsii, the causative agent of collar rot on Zamioculcas zamiifolia and an on farm method to mass produce Trichoderma species. Plant Pathology Journal, 9, 47–55.

Joko, T., Koentjoro, M. P., Somowiyarjo, S., Rohman, M. S., Liana, A., & Ogawa, N. (2012). Response of rhizobacterial communities in watermelon to infection with cucumber green mottle mosaic virus as revealed by cultivation-dependent RISA. Archives of Phytopathology and Plant Protection, 45(15), 1810–1818.

Karthikeyan, A., Kumar, S., & Kumar, S. (2003). Trichoderma viride: A mycoparasite for the control of Phytophthora cinnamomi. Indian Forester, 129(5), 631–634. Retrieved from

Khan, M. O., & Shahzad, S. (2007). Screening of Trichoderma species for tolerance to fungicides. Pakistan Journal of Botany, 39(3), 945–951. Retrieved from

Köprücü, K., & Seker, E. (2008). Acute toxicity of deltamethrin for freshwater mussel, Unio elongatulus eucirrus bourguignat. Bulletin of Environmental Contamination and Toxicology, 80(1), 1–4.

Lilly, V. G., & Barnett, H. L. (1951). Physiology of the fungi. New York, Toronto, London: McGraw-Hill Book Company, Inc. Retrieved from

Lo, C.-C. (2010). Effect of pesticides on soil microbial community. Journal of Environmental Science and Health Part. B, 45(5), 348–359.

Mahfut, Joko, T., & Daryono, B. S. (2016). Molecular characterization of odontoglossum ringspot virus (ORSV) in Java and Bali, Indonesia. Asian Journal of Plant Pathology, 10(1–2), 9–14.

Monte, E. (2001). Understanding Trichoderma: between biotechnology and microbial ecology. International Microbiology, 4(1), 1–4.

Oda, S. S., & El-Maddawy, Z. K. (2012). Protective effect of vitamin E and selenium combination on deltamethrin-induced reproductive toxicity in male rats. Experimental and Toxicologic Pathology, 64(7–8), 813–819.

Paret, M., Dufault, N., Momol, T., Marois, J., & Olson, S. (2015). Integrated disease management for vegetable crops in Florida. Florida: University of Florida. Retrieved from

Probowo, A., Prihatiningsih, N., & Soesanto, L. (2006). Potensi Trichoderma harzianum dalam mengendalikan sembilan isolat Fusarium oxysporum Schlecht. f. sp. zingiberi Trujillo pada kencur [Potency of Trichoderma harzianum in controlling nine isolates of Fusarium oxysporum Schlecht. f. sp. zingiberi Trujilo on galanga]. Jurnal Ilmu-Ilmu Pertanian Indonesia, 8(2), 76–84. Retrieved from

Reithner, B., Ibarra-Laclette, E., Mach, R. L., & Herrera-Estrella, A. (2011). Identification of mycoparasitism-related genes in Trichoderma atroviride. Applied and Environmental Microbiology, 77(13), 4361–4370.

Santoso, S. E., Soesanto, L., & Haryanto, T. A. D. (2007). Penekanan hayati penyakit moler pada bawang merah dengan Trichoderma harzianum, Trichoderma koningii, dan Pseudomonas fluorescens P60 [Biological suppression of moler disease on shallot by Trichoderma harzianum, Trichoderma koningii, and Pseudomonas fluorescens P60]. Jurnal Hama Dan Penyakit Tumbuhan Tropika, 7(1), 53–61. Retrieved from

Sengupta, D., Aktar, M. W., Alam, S., & Chowdhury, A. (2010). Impact assessment and decontamination of pesticides from meat under different culinary processes. Environmental Monitoring and Assessment, 169(1–4), 37–43.

Sharma, B. K., Singh, B. M., & Sugha, S. K. (1992). Integrated effect of biological and chemical control on sclerotial viability of Sclerotinia sclerotiorum (Lib.) de Bary. Journal of Biological Control, 6(1), 29–34. Retrieved from

Singh, V. P., Srivastava, S., Shrivastava, S. K., & Singh, H. B. (2012). Compatibility of different insecticides with Trichoderma harzianum under in vitro condition. Plant Pathology Journal, 11(2), 73–76.

Soesanto, L., Sudarmono, Prihatiningsih, N., Manan, A., Iriani, E., & Pramono, J. (2005). Potensi agensia hayati dan nabati dalam mengendalikan penyakit busuk rimpang jahe [Potency of biological and botanical agents in controlling ginger rhizome rot]. Jurnal Hama Dan Penyakit Tumbuhan Tropika, 5(1), 50–57. Retrieved from

Soytong, K., & Quyet, N. T. (2013). Production of organic compost from mushroom producing substances waste and tested for Kangkong organic cultivation. International Journal of Agricultural Technology, 9(1), 115–123. Retrieved from

Sutton, L. M., & Starzyk, M. J. (1972). Procedure and analysis of a useful method in determining mycelial dry weights from agar plates. Applied Microbiology, 24(6), 1011–1012. Retrieved from

Thakore, Y. (2006). The biopesticide market for global agricultural use. Industrial Biotechnology, 2(3), 194–208.

Vinale, F., Sivasithamparam, K., Ghisalberti, E. L., Marra, R., Woo, S. L., & Lorito, M. (2008). Trichoderma-plant-pathogen interactions. Soil Biology and Biochemistry, 40(1), 1–10.

Wardhana, D. W., Soesanto, L., & Utami, D. S. (2009). Penekanan hayati penyakit layu Fusarium pada subang gladiol [Biological suppression of Fusarial Wilt on gladiolus corms]. Jurnal Hortikultura, 19(2), 199–206. Retrieved from

Yang, C., Hamel, C., Vujanovic, V., & Gan, Y. (2011). Fungicide: Modes of action and possible impact on nontarget microorganisms. ISRN Ecology, 2011, 1–8.

Yildirim, M. Z., Benli, A. Ç. K., Selvi, M., Özkul, A., Erkoç, F., & Koçak, O. (2006). Acute toxicity, behavioral changes, and histopathological effects of deltamethrin on tissues (gills, liver, brain, spleen, kidney, muscle, skin) of Nile tilapia (Oreochromis niloticus L.) fingerlings. Environmental Toxicology, 21(6), 614–620.

Zimand, G., Elad, Y., & Chet, I. (1996). Effect of Trichoderma harzianum on Botrytis cinerea pathogenicity. Phytopathology, 86, 1255–1260.


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