Oviposition Deterrent of Bactrocera carambolae Resulted from Eggs Deposition on Mango

Muryati Muryati, Y. Andi Trisyono, Witjaksono Witjaksono, Wahyono Wahyono


Oviposition deterrent is chemical compounds which are used for avoiding eggs deposition. The oviposition deterrent resulted from eggs deposition is valuable information that can be manipulated for managing its population. The objective of this research was to determine the presence of oviposition deterrent resulted by female Bactrocera carambolae on mango. Extraction of oviposition deterrent was conducted by maceration method. The preference test was performed using two arms olfactometer, meanwhile the oviposition deterrent test was conducted by exposed gravid females to fruit that already smeared with extracts. The result revealed that gravid females of B. carambolae were attracted to methanol extract of 1 day after egg deposition, whereas the methanol extract of 3 and 5 days after egg deposition repelled gravid females. Oviposition deterrent test indicated that methanol extract at category 3 and 5 acted as a deterrent. The preference of gravid females of B. carambolae to different category of infested fruit extracts was probably influenced by the chemicals modification on mango after oviposition. This result suggested that the female B. carambolae do not deposit oviposition deterrent. The phenomenon of deterrence was probably as a result of chemical changes in fruit as a consequence of eggs infestation.


Bactrocera carambolae; fruit extract; mango; oviposition deterrent

Full Text:



Addesso, K. M., McAuslane, H. J., Stansly, P. A., & Schuster, D. J. (2007). Host-marking by female pepper weevils, Anthonomus eugenii. Entomologia Experimentalis et Applicata, 125(3), 269–276. http://doi.org/10.1111/j.1570-7458.2007.00626.x

Aluja, M., & Mangan, R. L. (2008). Fruit fly (Diptera: Tephritidae) host status determination: Critical conceptual, methodological, and regulatory considerations. Annual Review of Entomology, 53, 473–502. http://doi.org/10.1146/annurev.ento.53.103106.093350

Arredondo, J., & Diaz-Fleischer, F. (2006). Oviposition deterrents for the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae) from fly faeces extracts. Bulletin of Entomological Research, 96(1), 35–42. http://doi.org/10.1079/BER2005399

Averill, A. L., & Prokopy, R. J. (1989). Host marking pheromones. In A. S. Robinson & G. Hooper (Eds.), Fruit flies: Their biology, natural enemies and control (Vol. 3A, pp. 207-219). Amsterdam, NL: Elsevier Science Publishers.

Behar, A., Jurkevitch, E., & Yuval, B. (2008). Bringing back the fruit into fruit fly-bacteria interactions. Molecular Ecology, 17(5), 1375–1386. http://doi.org/10.1111/j.1365-294X.2008.03674.x

Bokhari, F., & Aly, M. M. (2009). Trials towards reduction of fungal growth and aflatoxin G1 production in Arabic coffee using different additives. African Journal of Food Science, 3(3), 068-076. Retrieved from http://www.academicjournals.org/article/article1380634716_Bokhari%20and%20Aly.pdf

Brévault, T., & Quilici, S. (2010). Interaction between visual and olfactory cues during host finding in the tomato fruit fly Neoceratitis cyanescens. Journal of Chemical Ecology, 36(3), 249–259. http://doi.org/10.1007/s10886-010-9766-6

Castells, E., & Berenbaum, M. R. (2008). Host plant selection by a monophagous herbivore is not mediated by quantitative changes in unique plant chemistry: Agonopterix alstroemeriana and Conium maculatum. Arthropod-Plant Interactions, 2, 43–51. http://doi.org/10.1007/s11829-008-9032-9

Clarke, A. R., Armstrong, K. F., Carmichael, A. E., Milne, J. R., Raghu, S., Roderick, G. K., & Yeates, D. K. (2005). Invasive phytophagous pests arising through a recent tropical evolutionary radiation: The Bactrocera dorsalis complex of fruit flies. Annual Review of Entomology, 50, 293-319. http://doi.org/10.1146/annurev.ento.50.071803.130428

Cornelius, M. L., Nergel, L., Duan, J. J., & Messing, R. H. (2014). Responses of female oriental fruit flies (Diptera: Tephritidae) to protein and host fruit odors in field cage and open field tests. Environmental Entomology, 29(1), 14-19. http://doi.org/10.1603/0046-225X-29.1.14

Cossé, A. A., Todd, J. L., Millar, J. G., Martínez, L. A., & Baker, T. C. (1995). Electroantennographic and coupled gas chromatographic-electroantennographic responses of the mediterranean fruit fly, Ceratitis capitata, to male-produced volatiles and mango odor. Journal of Chemical Ecology, 21(11), 1823–1836. http://doi.org/10.1007/BF02033679

Díaz-Fleischer, F., & Aluja, M. (2003). Influence of conspecific presence, experience, and host quality on oviposition behavior and clutch size determination in Anastrepha ludens (Diptera: Tephritidae). Journal of Insect Behavior, 16(4), 537–554. http://doi.org/10.1023/A:1027307424150

Genç, H. (2006). General principles of insect nutritional ecology. Trakya University Journal of Social Science, 7(1), 53-57. Retrieved from http://trakya.dergipark.gov.tr/download/article-file/213755

Jang, E. B., & Light, D. M. (1991). Behavioral responses of female oriental fruit flies to the odor of papayas at three ripeness stages in a laboratory flight tunnel (Diptera: Tephritidae). Journal of Insect Behavior, 4(6), 751–762. http://doi.org/10.1007/BF01052229

Jeyasankar, A. (2009). Chemical ecology of fruit flies management - A review. Journal of Basic and Applied Biology, 3, 1-5.

Joomaye, A., & Price, N. S. (1999). Pest risk analysis and quarantine of fruit flies in the Indian Ocean region. Quatre Bornes, MU: Ministry of Agriculture, Food Technology and Natural Resources.

Katsoyannos, B. I., Kouloussis, N. A., & Papadopoulos, N. T. (1997). Response of Ceratitis capitata to citrus chemicals under semi-natural conditions. Entomologia Experimentalis et Applicata, 82(2), 181–188. http://doi.org/10.1046/j.1570-7458.1997.00129.x

Kramer, W. L., & Mulla, M. S. (1979). Oviposition attractants and repellents of mosquitoes: Oviposition responses of culex mosquitoes to organic infusions. Environmental Entomology, 8(6), 1111–1117. http://doi.org/10.1093/ee/8.6.1111

Li, G., & Ishikawa, Y. (2005). Oviposition deterrents from the egg masses of the adzuki bean borer, Ostrinia scapulalis and Asian corn borer, O. furnacalis. Entomologia Experimentalis et Applicata, 115(3), 401-407. http://doi.org/10.1111/j.1570-7458.2005.00282.x

Light, D. M., & Jang, E. B. (1987). Electroantennogram responses of the oriental fruit fly, Dacus dorsalis, to a spectrum of alcohol and aldehyde plant volatiles. Entomologia Experimentalis et Applicata, 45(1), 55-64. http://doi.org/10.1111/j.1570-7458.1987.tb02255.x

Manrakhan, A., & Lux, S. A. (2008). Effect of food deprivation on attractiveness of food sources, containing natural and artificial sugar and protein, to three African fruit flies: Ceratitis cosyra, Ceratitis fasciventris, and Ceratitis capitata. Entomologia Experimentalis et Applicata, 127(2), 133-143. http://doi.org/10.1111/j.1570-7458.2008.00686.x

Marchand, D., & McNeil, J. N. (2004). Avoidance of intraspecific competition via host modification in a grazing, fruit-eating insect. Animal Behaviour, 67(3), 397–402. http://doi.org/10.1016/j.anbehav.2003.03.017

Meagher, R. L., & Landolt, P. J. (2008). Attractiveness of binary blends of floral odorant compounds to moths in Florida, USA. Entomologia Experimentalis et Applicata, 128, 323–329. http://doi.org/10.1111/j.1570-7458.2008.00711.x

Messina, F. J., Barmore, J. L., & Renwick, J. A. A. (1987). Oviposition deterrent from eggs of Callosobruchus maculatus: Spacing mechanism or artifact? Journal of Chemical Ecology, 13(1), 219–226. http://doi.org/10.1007/BF01020364

Muryati, Hasyim, A., & Riska. (2008). Preferensi spesies lalat buah terhadap atraktan metil eugenol dan cue-lure dan populasinya di Sumatera Barat dan Riau [The preference of fruitflies species to methyl eugenol and cue-lure attractant and its population in West Sumatera and Riau]. Jurnal Hortikultura, 18(2), 227-233. http://doi.org/10.21082/jhort.v18n2.2008.p%25p

Muryati, Trisyono, Y. A., Witjaksono, & Wahyono. (2012). Effects of citronella grass extract on the oviposition behavior of carambola fruit fly (Bactrocera carambolae) in mango. ARPN Journal of Agricultural and Biological Science, 7(9), 672-679. Retrieved from http://www.arpnjournals.com/jabs/research_papers/rp_2012/jabs_0912_452.pdf

Niogret, J., Montgomery, W. S., Kendra, P. E., Heath, R. R., & Epsky, N. D. (2011). Attraction and electroantennogram responses of male Mediterranean fruit fly to volatile chemicals from Persea, Litchi and Ficus wood. Journal of Chemical Ecology, 37, 483-491. http://doi.org/10.1007/s10886-011-9953-0

Norin, T. (2007). Semiochemicals for insect pest management. Pure and Applied Chemistry, 79(12), 2129–2136. http://doi.org/10.1351/pac200779122129

Nufio, C. R., & Papaj, D. R. (2001). Host marking behavior in phytophagous insects and parasitoids. Entomologia Experimentalis et Applicata, 99, 273–293. Retrieved from http://eebweb.arizona.edu/papaj/Pdfs/Nufio & Papaj, MPReview.pdf

Nufio, C. R., & Papaj, D. R. (2004). Host-marking behaviour as a quantitative signal of competition in the walnut fly Rhagoletis juglandis. Ecological Entomology, 29(3), 336–344. http://doi.org/10.1111/j.1365-2311.2004.00607.x

Paiva, P. M. G., Gomes, F. S., Napoleão, T. H., Sa, R. A., Correia, M. T. S., & Coelho, L. C. B. B. (2010). Antimicrobial activity of secondary metabolites and lectins from plants. In A. Mendez-Vilas (Ed.), Current research, technology and education topics in applied microbiology and microbial biotechnology (1st ed., pp. 396–406). Badajoz, ES: Formatex Research Center. Retrieved from http://www.formatex.info/microbiology2/396-406.pdf

Papadopoulos, N. T., Kouloussis, N. A., & Katsoyannos, B. I. (2006). Effect of plant chemicals on the behavior of the Mediterranean fruit fly. In Fruit flies of economic importance: From basic to applied knowledge. Paper presented at Proceedings of the 7th International Symposium on Fruit Flies of Economic Importance, 10-15 September 2006, Salvador, Brazil (pp. 97-106). Retrieved from http://www.iaea.org/inis/collection/NCLCollectionStore/_Public/42/109/42109314.pdf

Papaj, D. R., & Messing, R. H. (1996). Functional shifts in the use of parasitized hosts by a tephritid fly: The role of host quality. Behavioral Ecology, 7(3), 235–242. http://doi.org/10.1093/beheco/7.3.235

Parekh, J., Jadeja, D., & Chanda, S. (2005). Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turkish Journal of Biology, 29, 203–210. Retrieved from http://citeseerx.ist.psu.edu/viewdoc/download?doi=

Patt, J. M., & Pfannenstiel, R. S. (2008). Odor-based recognition of nectar in cursorial spiders. Entomologia Experimentalis et Applicata, 127(1), 64–71. http://doi.org/10.1111/j.1570-7458.2008.00669.x

Pinero, J. C., & Dorn, S. (2007). Synergism between aromatic compounds and green leaf volatiles derived from the host plant underlies female attraction in the oriental fruit moth. Entomologia Experimentalis et Applicata, 125(2), 185-194. http://doi.org/10.1111/j.1570-7458.2007.00614.x

Powell, G., Tosh, C. R., & Hardie, J. (2006). Host plant selection by aphids: Behavioral, evolutionary, and applied perspectives. Annual Review of Entomology, 51, 309–330. http://doi.org/10.1146/annurev.ento.51.110104.151107

Ravikumar, P., & Viraktamath, S. (2007). Attraction of female fruit flies to different protein food baits in guava and mango orchards. Karnataka Journal of Agricultural Sciences, 20(4), 745-748. Retrieved from

Riffell, J. A., Abrell, L., & Hildebrand, J. G. (2008). Physical processes and real-time chemical measurement of the insect olfactory environment. Journal of Chemical Ecology, 34(7), 837–853. http://doi.org/10.1007/s10886-008-9490-7

Robacker, D. C. (2007). Chemical ecology of bacterial relationships with fruit flies. IOBC-WPRS Bulletins, 30(9), 9–22. Retrieved from https://naldc.nal.usda.gov/download/20062/PDF

Roitberg, B. D., & Mangel, M. (1988). On the evolutionary ecology of marking pheromones. Evolutionary Ecology, 2(4), 289–315. http://doi.org/10.1007/BF02207562

Rull, J., Prokopy, R. J., & Vargas, R. I. (2003). Effects of conspecific presence on arrival and use of hosts in Ceratitis capitata flies. Journal of Insect Behavior, 16(3), 329–346. http://doi.org/10.1023/A:1024871908372

Růžička, Z., & Havelka, J. (1998). Effects of oviposition-deterring pheromone and allomones on Aphidoletes aphidimyza (Diptera: Cecidomyiidae). European Journal of Entomology, 95, 211–216. Retrieved from http://www.eje.cz/pdfs/eje/1998/02/04.pdf

Sakai, A., Honda, H., Oshima, K., & Yamamoto, I. (1986). Oviposition marking pheromone of two bean weevils, Callosobruchus chinensis and Callosobruchus maculatus. Journal of Pesticide Science, 11(2), 163-168. http://doi.org/10.1584/jpestics.11.163

Schoonhoven, L. M., Sparnaay, T., van Wissen, W., & Meerman, J. (1981). Seven-week persistence of an oviposition-deterrent pheromone. Journal of Chemical Ecology, 7(3), 583-588. http://doi.org/10.1007/BF00987706

Siderhurst, M. S., & Jang, E. B. (2010). Cucumber volatile blend attractive to female melon fly, Bactrocera cucurbitae (Coquillett). Journal of Chemical Ecology, 36(7), 699–708. http://doi.org/10.1007/s10886-010-9804-4

Sood, P., & Nath, A. (2005). Colonization of marker strains of bacteria in fruit fly, Bactrocera tau. Indian Journal Of Agricultural Research, 39(2), 103-109. Retrieved from http://www.arccjournals.com/uploads/articles/ijar2392004.pdf

Stelinski, L. L., Zhang, A., Onagbola, E. O., & Meyer, W. L. (2009). Recognition of foreign oviposition marking pheromones is context dependent and determined by preimaginal conditioning. Communicative and Integrative Biology, 2(5), 391–393. http://doi.org/10.4161/cib.2.5.8759

Thiery, D., & Le Quere, J. L. (1991). Identification of an oviposition-deterring pheromone in the eggs of the European corn borer. Naturwissenschaflen, 78, 132-133. Retrieved from https://www.researchgate.net/publication/226717695_Identification_of_an_oviposition-deterring_pheromone_in_the_eggs_of_the_European_corn_borer

Thompson, J. N., & Pellmyr, O. (1991). Evolution of oviposition behavior and host preference in lepidoptera. Annual Review of Entomology, 36, 65–89. http://doi.org/10.1146/annurev.ento.36.1.65

van Sauers-Muller, A. (2005). Host plants of the carambola fruit fly, Bactrocera carambolae Drew & Hancock (Diptera: Tephritidae), in Suriname, South America. Neutropical Entomology, 34(2), 203–214. http://doi.org/10.1590/S1519-566X2005000200008

Wang, Y., & Kays, S. J. (2002). Sweetpotato volatile chemistry in relation to sweetpotato Weevil (Cylas formicarius) behavior. Journal of the American Society for Horticultural Science, 127(4), 656-662. Retrieved from http://journal.ashspublications.org/content/127/4/656.full.pdf

White, I. M., & Hancock, D. L. (1997). Cabikey to the Indo-Australian dacini fruit flies [CD ROM]. Oxfordshire, UK: CABI Publishing.

DOI: http://doi.org/10.17503/agrivita.v39i2.1097

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.