Effects of Sucrose and Plant Hormone on the Pigmentation of Mesocarp of White- and Red-Fleshed Peach Fruits

Inna Martha Rumainum, Kanjana Worarad, Yoshikazu Yamaki, Kenji Yamane

Abstract


Three cultivars of peach (Prunuspersica L. Batsch): ‘Ikeda’, ‘Akatsuki’ (white-fleshed) and ‘Tenshin Suimitsuto’ (red-fleshed) were used to study the effect of sucrose and auxin on the pigmentation of mesocarp. In the first experiment, mesocarp discs of ‘Ikeda’ and ‘Tenshin Suimitsuto’ were incubated on solidified MS medium containing sucrose, 1-naphtalene acetic acid (NAA), and their combination. A treatment of 10μM NAA increased the total of anthocyanin content in ‘Ikeda’. In ‘Tenshin Suimitsuto’, a treatment of 100 mM sucrose increased the total of anthocyanin content. In the second experiment, mesocarp discs of ‘Akatsuki’ and ‘Tenshin Suimitsuto’ were employed to examine the effect of 2,3,5-triiodobenzoic acetic acid (TIBA). The mesocarp discs were incubated on solidified MS medium containing NAA or combination of NAA and TIBA. A single TIBA treatment was applied in ‘Tenshin Suimitsuto’. TIBA treatment significantly (P<0.05) reduced the total anthocyanin content in both cultivars. The total phenolic content was decreased by TIBA treatment in both cultivars. A treatment of single TIBA (200 mM) significantly (P<0.05) inhibited anthocyanin, flavonoid and phenolic accumulation in ‘Tenshin Suimitsuto’. Present study shows that sucrose and auxin might regulate anthocyanin synthesis, as well as phenolic compounds, in the flesh of peach fruit.


Keywords


Anthocyanin; NAA; Peach; Pigmentation; TIBA

Full Text:

PDF

References


Cantín, C. M., Moreno, M. A., & Gogorcena, Y. (2009). Evaluation of the antioxidant capacity, phenolic compounds, and vitamin C content of different peach and nectarine [Prunus persica (L.) batsch] breeding progenies. Journal of Agricultural and Food Chemistry, 57(11), 4586–4592. crossref

Carvalho, R. F., Quecini, V., & Peres, L. E. P. (2010). Hormonal modulation of photomorphogenesis-controlled anthocyanin accumulation in tomato (Solanum lycopersicum L. cv Micro-Tom) hypocotyls: Physiological and genetic studies. Plant Science, 178(3), 258–264. crossref

Çelik, H., Özgen, M., Serçe, S., & Kaya, C. (2008). Phytochemical accumulation and antioxidant capacity at four maturity stages of cranberry fruit. Scientia Horticulturae, 117(4), 345–348. crossref

Christie, A. E., & Leopold, A. C. (1965). On the manner of triiodobenzoic acid inhibition of auxin transport. Plant and Cell Physiology, 6(2), 337–345. crossref

Dai, Z.-W., Meddar, M., Renaud, C., Merlin, I., Hilbert, G., Delro, S., & Gomès, E. (2014). Long-term in vitro culture of grape berries and its application to assess the effects of sugar supply on anthocyanin accumulation. Journal of Experimental Botany, 65(16), 4665–4677. crossref

Falchi, R., Zanon, L., De Marco, F., Nonis, A., Pfeiffer, A., & Vizzotto, G. (2013). Tissue-specific and developmental expression pattern of abscisic acid biosynthetic genes in peach fruit: Possible role of the hormone in the coordinated growth of seed and mesocarp. Journal of Plant Growth Regulation, 32(3), 519–532. crossref

Fikrinda, W., Susanto, S., Efendi, D., & Melati, M. (2015). Study on fruit quality of selected seeded pummelo cultivars and its relationship with antioxidant activity content during storage period. Agrivita Journal of Agricultural Science, 37(3), 210–219. crossref

Given, N. K., Venis, M. A., & Gierson, D. (1988). Hormonal regulation of ripening in the strawberry, a non-climacteric fruit. Planta, 174(3), 402–406. crossref

Hikosaka, S., & Sugiyama, N. (2015). Effects of exogenous plant growth regulators on yield, fruit growth, and concentration of endogenous hormones in gynoecious parthenocarpic cucumber (Cucumis sativus L.). Horticulture Journal, 84(4), 342–349. crossref

Jiao, Y., Ma, R. J., Shen, Z. J., Yan, J., & Yu, M. L. (2014). Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development. Journal of Zhejiang University-Science B, 15(9), 809–819. crossref

Lewis, D. R., Ramirez, M. V., Miller, N. D., Vallabhaneni, P., Ray, W. K., Helm, R. F., … Muday, G. K. (2011). Auxin and ethylene induce flavonol accumulation through distinct transcriptional networks. Plant Physiology, 156(1), 144–164. crossref

Manganaris, G. A., Goulas, V., Vicente, A. R., & Terry, L. A. (2014). Berry antioxidants: Small fruits providing large benefits. Journal of the Science of Food and Agriculture. crossref

Martínez, C., Manzano, S., Megías, Z., Garrido, D., Picó, B., & Jamilena, M. (2013). Involvement of ethylene biosynthesis and signalling in fruit set and early fruit development in zucchini squash (Cucurbita pepo L.). BMC Plant Biology, 13(1), 139. crossref

Ohmiya, A. (2000). Effects of auxin on growth and ripening of mesocarp discs of peach fruit. Scientia Horticulturae, 84(3–4), 309–319. crossref

Rumainum, I. M. (2016). Studies on the accumulation of flavonoid and carotenoid in peach fruits. Dissertation Abstracts International, 03,https://tuat.repo.nii.ac.jp/?action=repository_action_common_download&item_id=1322&item_no=1&attribute_id=16&file_no=1

Rumainum, I. M., Worarad, K., Yamaki, Y., & Yamane, K. (2016). Effects of developmental stages, light, and an auxin polar transport inhibitor on the skin and flesh pigmentation of red-fleshed peach fruit. The Horticulture Journal, 85(2), 141–147. crossref

Tadiello, A., Ziosi, V., Negri, A. S., Noferini, M., Fiori, G., Busatto, N., … Trainotti, L. (2016). On the role of ethylene, auxin and a GOLVEN-like peptide hormone in the regulation of peach ripening. BMC Plant Biology, 16(1), 44. crossref

Tuan, P. A., Bai, S., Yaegaki, H., Tamura, T., Hihara, S., Moriguchi, T., & Oda, K. (2015). The crucial role of PpMYB10.1 in anthocyanin accumulation in peach and relationships between its allelic type and skin color phenotype. BMC Plant Biology, 15(1), 280. crossref

Yu, Z., Lirong, W., Wei, C., Gengrui, Z., Weichao, F., Changwen, C., & Futian, P. (2013). Genetic diversity of anthocyanin in peach fruit and the evaluating criterion of red-flesh peach. Journal of Plant Genetic Resources, 14(1), 169–174. Retrieved from website

Zhang, Q.-P., Li, J., Wang, L.-R., Zhu, G.-R., Fang, W-C., Cao, K., Chen, C.-W., Feng, Y.-B. (2008). Study on the changes of contents of pigments, sugar and acid of blood-flesh peach cultivar during fruit development. Journal of Fruit Science, 25(3), 312-315. Retrieved from website

Zheng, Y., Tian, L., Liu, H., Pan, Q., Zhan, J., & Huang, W. (2009). Sugars induce anthocyanin accumulation and flavanone 3-hydroxylase expression in grape berries. Plant Growth Regulation, 58(3), 251–260. crossref




DOI: http://doi.org/10.17503/agrivita.v40i2.1094

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.